Filter-based PNA in situ hybridization for rapid detection, identification and enumeration of specific micro-organisms.

Abstract:

AIMS:A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. METHODS AND RESULTS:The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of time. The microcolonies were analysed by in situ hybridization, using peroxidase-labelled PNA probes targeting a species-specific rRNA sequence, and visualized by a chemiluminescent reaction. Microcolonies were observed as small spots of light on film, thereby providing simultaneous detection, identification and enumeration. The method showed 95-100% correlation to standard plate counts along with definitive identification due to the specificity of the probe. CONCLUSION:Using the same protocol, results were generated approximately three times faster than culture methods for Gram-positive and -negative bacterial species and yeast species. SIGNIFICANCE AND IMPACT OF THE STUDY:The method is an improvement on the current membrane filtration technique, providing rapid determination of the level of specific pathogens, spoilage or indicator micro-organisms.

journal_name

J Appl Microbiol

authors

Perry-O'Keefe H,Stender H,Broomer A,Oliveira K,Coull J,Hyldig-Nielsen JJ

doi

10.1046/j.1365-2672.2001.01230.x

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

180-9

issue

2

eissn

1364-5072

issn

1365-2672

pii

jam1230

journal_volume

90

pub_type

杂志文章
  • Influence of growth phases and desiccation on the degrees of unsaturation of fatty acids and the survival rates of rhizobia

    abstract::The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00860.x

    authors: Boumahdi M,Mary P,Hornez J

    更新日期:1999-10-01 00:00:00

  • Molecular characterization of Oenococcus oeni genes encoding proteins involved in arginine transport.

    abstract:AIMS:This work was carried out to complete the sequence of the arc cluster involved in arginine catabolism in Oenococcus oeni, and particularly to characterize the genes encoding proteins involved in arginine transport. METHODS AND RESULTS:Using molecular cloning, two loci encoding proteins involved in the arginine-or...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01907.x

    authors: Divol B,Tonon T,Morichon S,Gindreau E,Lonvaud-Funel A

    更新日期:2003-01-01 00:00:00

  • Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes.

    abstract::The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01054.x

    authors: Wang Y,McAllister TA,Yanke LJ,Cheeke PR

    更新日期:2000-05-01 00:00:00

  • Occurrence of Helicobacter pylori in surface water in the United States.

    abstract::The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water-borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively r...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00912.x

    authors: Hegarty JP,Dowd MT,Baker KH

    更新日期:1999-11-01 00:00:00

  • Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt.

    abstract:AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04574.x

    authors: Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

    更新日期:2010-04-01 00:00:00

  • Streptomyces effect on the bacterial microbiota associated to Crassostrea sikamea oyster.

    abstract:AIM:To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS:DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richnes...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13382

    authors: García Bernal M,Trabal Fernández N,Saucedo Lastra PE,Medina Marrero R,Mazón-Suástegui JM

    更新日期:2017-03-01 00:00:00

  • Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains.

    abstract:AIM:Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of CLEs and their combinations agains...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14070

    authors: Nikolić I,Berić T,Dimkić I,Popović T,Lozo J,Fira D,Stanković S

    更新日期:2019-01-01 00:00:00

  • Antibacterial activity of Litsea cubeba (Lauraceae, May Chang) and its effects on the biological response of common carp Cyprinus carpio challenged with Aeromonas hydrophila.

    abstract:AIMS:The aims of this study were to characterize the antibacterial activity and the chemotype of Litsea cubeba leaf essential oil (EO) harvested in North Vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (Cyprinus carpio) challenged ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13160

    authors: Nguyen HV,Caruso D,Lebrun M,Nguyen NT,Trinh TT,Meile JC,Chu-Ky S,Sarter S

    更新日期:2016-08-01 00:00:00

  • Physiology and taxonomy of thiobacillus strain TJ330, which oxidizes carbon disulphide (CS2).

    abstract::A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01150.x

    authors: Hartikainen T,Ruuskanen J,Räty K,Von Wright A,Martikainen PJ

    更新日期:2000-10-01 00:00:00

  • Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells.

    abstract:AIMS:The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. METHOD AND RESULTS:We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic acti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03615.x

    authors: Jeong SY,Park SY,Kim YH,Kim M,Lee SJ

    更新日期:2008-03-01 00:00:00

  • A sensitive method for the recovery of Escherichia coli serogroup O55 including Shiga toxin-producing variants for potential use in outbreaks.

    abstract:AIM:Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by the availability of sensitive methods for detecting these pathogens. In this study, we developed novel too...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14345

    authors: Kirchner M,Sayers E,Cawthraw S,Duggett N,Gosling R,Jenkins C,Dallman TJ,Mueller-Doblies D,Anjum MF

    更新日期:2019-09-01 00:00:00

  • Phages of enteric bacteria in fresh water with different levels of faecal pollution.

    abstract::Levels of somatic and F-specific coliphages, and phages infecting Bacteroides fragilis were measured in 257 samples collected in different freshwater environments with different levels and characteristics of faecal pollution. In samples with recent pollution of domestic origin, the numbers of the three groups of phage...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00354.x

    authors: Araujo RM,Puig A,Lasobras J,Lucena F,Jofre J

    更新日期:1997-03-01 00:00:00

  • Inactivation of Escherichia coli by citral.

    abstract:AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04597.x

    authors: Somolinos M,García D,Condón S,Mackey B,Pagán R

    更新日期:2010-06-01 00:00:00

  • Genetic correlation between chromium resistance and reduction in Bacillus brevis isolated from tannery effluent.

    abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04326.x

    authors: Verma T,Garg SK,Ramteke PW

    更新日期:2009-11-01 00:00:00

  • Updating the H-antigen classification of Bacillus thuringiensis.

    abstract::The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00710.x

    authors: Lecadet MM,Frachon E,Dumanoir VC,Ripouteau H,Hamon S,Laurent P,Thiéry I

    更新日期:1999-04-01 00:00:00

  • Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, Lact. fermentum, Lact. rhamnosus, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weissella minor and related taxa isolated from kocho

    abstract:AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01202.x

    authors: Nigatu A

    更新日期:2000-12-01 00:00:00

  • Immunostimulatory activities of specific bacterial secondary metabolite of Anoxybacillus flavithermus strain SX-4 on carp, Cyprinus carpio.

    abstract:AIMS:  To determine the capacity of secondary metabolite of strain SX-4, to enhance the nonspecific immunity and survival of carp (Cyprinus carpio), and to identify the constituents that are responsible. METHODS AND RESULTS:  A thermophilic strain SX-4 that is able to produce immunostimulatory metabolite was isolated ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04963.x

    authors: Liu J,Lei Y,Wang F,Yi Y,Liu Y,Wang G

    更新日期:2011-04-01 00:00:00

  • Production and characterization of a thermostable chitinase from a new alkalophilic Bacillus sp. BG-11.

    abstract::An alkalophilic, environmental micro-organism, Bacillus sp. BG-11, has been isolated and characterized. It produced 76 U ml-1 of chitinase in liquid batch fermentation after 72 h of incubation at 50 degrees C using chitin-enriched medium. The molecular weight of purified chitinase was estimated to be 41 kDa by SDS-PAG...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01016.x

    authors: Bhushan B

    更新日期:2000-05-01 00:00:00

  • The effect of carbon and nitrogen sources on bovicin HC5 production by Streptococcus bovis HC5.

    abstract:AIMS:To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS:Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04212.x

    authors: De Carvalho AA,Mantovani HC,Paiva AD,De Melo MR

    更新日期:2009-07-01 00:00:00

  • Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica.

    abstract::A number of phenotypic and molecular fingerprinting techniques, including physiological profiling (Biolog), restriction fragment length polymorphism (RFLP), enterobacterial repetitive intergenic consensus (ERIC) and a phage typing system, were evaluated for their ability to differentiate between 60 strains of Erwinia ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00929.x

    authors: Toth IK,Bertheau Y,Hyman LJ,Laplaze L,López MM,McNicol J,Niepold F,Persson P,Salmond GP,Sletten A,van Der Wolf JM,Pérombelon MC

    更新日期:1999-11-01 00:00:00

  • Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease.

    abstract:AIM:To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease. METHODS AND RESULTS:Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and fiel...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03611.x

    authors: Haggag WM,Timmusk S

    更新日期:2008-04-01 00:00:00

  • Combination of antagonistic yeasts with two food additives for control of brown rot caused by Monilinia fructicola on sweet cherry fruit.

    abstract:AIMS:To evaluate beneficial effect of two food additives, ammonium molybdate (NH4-Mo) and sodium bicarbonate (NaBi), on antagonistic yeasts for control of brown rot caused by Monilinia fructicola in sweet cherry fruit under various storage conditions. The mechanisms of action by which food additives enhance the efficac...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02821.x

    authors: Qin GZ,Tian SP,Xu Y,Chan ZL,Li BQ

    更新日期:2006-03-01 00:00:00

  • A modified indirect mathematical model for evaluation of ethanol production efficiency in industrial-scale continuous fermentation processes.

    abstract:AIMS:To calculate fermentation efficiency in a continuous ethanol production process, we aimed to develop a robust mathematical method based on the analysis of metabolic by-product formation. METHODS AND RESULTS:This method is in contrast to the traditional way of calculating ethanol fermentation efficiency, where the...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13240

    authors: Canseco Grellet MA,Castagnaro A,Dantur KI,De Boeck G,Ahmed PM,Cárdenas GJ,Welin B,Ruiz RM

    更新日期:2016-10-01 00:00:00

  • Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: mechanisms involved and potential as a biocontrol agent.

    abstract:AIMS:To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. METHODS AND RESULTS:The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual c...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04970.x

    authors: Rodríguez MA,Cabrera G,Gozzo FC,Eberlin MN,Godeas A

    更新日期:2011-05-01 00:00:00

  • Diversity among Bradyrhizobium isolates nodulating yardlong bean and sunnhemp in Guam.

    abstract:AIMS:To isolate and characterize bradyrhizobia that nodulate yardlong bean and sunnhemp in Guam. METHODS AND RESULTS:Bradyrhizobia populations that nodulate yardlong bean and sunnhemp in Guam were examined for genetic diversity and their relatedness to Bradyrhizobium japonicum and B. elkanii reference strains. Genomic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01733.x

    authors: You Z,Marutani M,Borthakur D

    更新日期:2002-01-01 00:00:00

  • Purification and partial characterization of a bacteriocin produced by Eikenella corrodens.

    abstract:AIMS:The purpose of this study was to purify and characterize a bacteriocin produced by Eikenella corrodens A32E2. METHODS AND RESULTS:Peptostreptococcus anaerobius ATCC27337 was used as indicator strain in antagonistic assays for bacteriocin-producing E. corrodens A32E2. Protein extraction was influenced by pH and bu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03565.x

    authors: Apolônio AC,Carvalho MA,Bemquerer MP,Santoro MM,Pinto SQ,Oliveira JS,Santos KV,Farias LM

    更新日期:2008-02-01 00:00:00

  • Membrane disruption and anti-quorum sensing effects of synergistic interaction between Lavandula angustifolia (lavender oil) in combination with antibiotic against plasmid-conferred multi-drug-resistant Escherichia coli.

    abstract:AIM:The aim of this study was to investigate the mode of action of the lavender essential oil (LV) on antimicrobial activity against multi-drug-resistant Escherichia coli J53 R1 when used singly and in combination with piperacillin. METHOD AND RESULTS:In the time-kill analysis, a complete killing of bacteria was obser...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12444

    authors: Yap PS,Krishnan T,Yiap BC,Hu CP,Chan KG,Lim SH

    更新日期:2014-05-01 00:00:00

  • A feed additive containing Bacillus toyonensis (Toyocerin(®) ) protects against enteric pathogens in postweaning piglets.

    abstract:AIMS:This study evaluated the efficacy of a probiotic containing Bacillus toyonensis spores (Toyocerin(®) ) in postweaning piglets against enteric pathogens. METHODS AND RESULTS:Seven hundred and ninety-two healthy weaning pigs of a commercial farrow-to-finish pig farm were used. The negative control group fed without...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/jam.12729

    authors: Kantas D,Papatsiros VG,Tassis PD,Giavasis I,Bouki P,Tzika ED

    更新日期:2015-03-01 00:00:00

  • Genotypic and phenotypic heterogeneity among lactococci isolated from traditional Pecorino Sardo cheese.

    abstract::Twenty-nine Lactococcus lactis isolates from one traditional 24 h-old Pecorino Sardo cheese were characterized phenotypically, technologically and genotypically in order to assess the biodiversity within this wild microbial population. Two DNA-based techniques, plasmid profiling and PFGE, were used for the genetic typ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01109.x

    authors: Mannu L,Paba A,Pes M,Scintu MF

    更新日期:2000-08-01 00:00:00

  • Nutritive value of treated Quercus infectoria and Quercus libani leaves with the tannin-degrading bacterium Klebsiella pneumoniae for ruminant feeding in vitro.

    abstract:AIMS:This study was conducted to evaluate the chemical composition and in vitro gas production (GP) and fermentation parameters of Quercus infectoria and Quercus libani leaves following treatment with the Klebsiella pneumoniae, a tannin-degrading bacterium. METHODS AND RESULTS:This isolate was isolated on medium conta...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14408

    authors: Motamedi H,Azizi A,Ahmadi M

    更新日期:2019-11-01 00:00:00