Abstract:
AIMS:The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. METHOD AND RESULTS:We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic activity on three colon cancer cells (HT-29, SW480 and HCT116). Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify BIT-33 as a strain of Bacillus vallismortis. The cytotoxic compound from B. vallismortis BIT-33 was purified by reverse-phase high-performance liquid chromatography. Direct cytotoxic effect of the compound was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay. The compound induced apoptosis of colon cancer cells, as indicated by DNA fragmentation of agarose gel electrophoresis, flow cytometric analysis (sub-G(1) method) and annexin V staining. CONCLUSION:The cytotoxic compound from B. vallismortis BIT-33 was purified, and the compound showed direct cytotoxic and apoptotic effects on colon cancer cells in a dose- and time-dependent manner. SIGNIFICANCE AND IMPACT OF THE STUDY:Taken together, our results suggest that the compound from B. vallismortis BIT-33 could be a candidate for the development of apoptosis-specific anti-tumour agents. This study indicated that marine bacteria could be an important source of cytotoxic metabolites.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Jeong SY,Park SY,Kim YH,Kim M,Lee SJdoi
10.1111/j.1365-2672.2007.03615.xsubject
Has Abstractpub_date
2008-03-01 00:00:00pages
796-807issue
3eissn
1364-5072issn
1365-2672pii
JAM3615journal_volume
104pub_type
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