Abstract:
AIMS:Quantitative polymerase chain reaction (QPCR) methods for beach monitoring by estimating abundance of Enterococcus spp. in recreational waters use internal, positive controls which address only the amplification of target DNA. In this study two internal, positive controls were developed to control for both amplification and cell lysis in assays measuring abundance of vegetative Gram-positive bacteria. METHODS AND RESULTS:Controls were constructed using Streptococcus gordonii DL-1, a naturally transformable, Gram-positive bacterium. Unique target sequences were provided by chromosomal insertion of a genetically modified, green fluorescent protein gene fragment. Results suggest that their use for control of lysis and amplification may be of significant value. CONCLUSIONS:The use of these controls and the establishment of data quality objectives to determine the tolerable level of decision error should ensure that environmental decisions based on QPCR data are technically and scientifically sound. SIGNIFICANCE AND IMPACT OF THE STUDY:QPCR measurements related to cell abundance may vary between samples as thick-walled Gram-positive bacteria are inherently difficult to lyse and substances present in recreational waters may inhibit amplification. As QPCR methods are considered for beach monitoring, it is essential to demonstrate that the data obtained accurately reflects the abundance of the bacterial indicator.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
James JB,Genthner FJdoi
10.1111/j.1365-2672.2008.03936.xsubject
Has Abstractpub_date
2008-12-01 00:00:00pages
2213-22issue
6eissn
1364-5072issn
1365-2672pii
JAM3936journal_volume
105pub_type
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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abstract:AIMS:Chlorophyll is a photosynthetic pigment found in plants and algal organisms and is a bioproduct with human health benefits and a great potential for use in the food industry. The chlorophyll content in microalgae strains varies in response to environmental factors. In this work, we assessed the effect of nitrogen ...
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2007.03580.x
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2004.02491.x
更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2007-10-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00375.x
更新日期:1998-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01561.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To investigate the influence of several environmental factors on the viability and cell-adsorption for two Lactobacillus casei/paracasei bacteriophages (PL-1 and J-1). METHODS AND RESULTS:Both phages showed a remarkably high specificity of species, sharing similar host spectra. Two phages and four sensitive strain...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02767.x
更新日期:2006-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01771.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02289.x
更新日期:2004-01-01 00:00:00
abstract::A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...
journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.01150.x
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2001.01263.x
更新日期:2001-03-01 00:00:00
abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04411.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the antibacterial activity and the chemotype of Litsea cubeba leaf essential oil (EO) harvested in North Vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (Cyprinus carpio) challenged ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13160
更新日期:2016-08-01 00:00:00
abstract::The goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13438
更新日期:2017-07-01 00:00:00
abstract:AIMS:Point-of-use drinking water disinfection with sodium hypochlorite has been shown to improve water quality and reduce diarrhoeal disease. However, the chlorine demand of highly turbid water may render sodium hypochlorite less effective. METHODS AND RESULTS:We evaluated a novel combined flocculant-disinfectant poin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02309.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Microbial community associated with hydrogen production and volatile fatty acids (VFAs) accumulation was characterized in acidogenic hydrogenesis using molasses wastewater as a feedstock. METHODS AND RESULTS:Hydrogen and VFAs production were measured under an organic loading rate (OLR) from 19 to 35 g-COD l-1 da...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13316
更新日期:2016-12-01 00:00:00
abstract:AIMS:The purpose of this study was to purify and characterize a bacteriocin produced by Eikenella corrodens A32E2. METHODS AND RESULTS:Peptostreptococcus anaerobius ATCC27337 was used as indicator strain in antagonistic assays for bacteriocin-producing E. corrodens A32E2. Protein extraction was influenced by pH and bu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03565.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14790
更新日期:2020-07-20 00:00:00
abstract:AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02480.x
更新日期:2005-01-01 00:00:00
abstract::Staphylococcus aureus is an important agent of contagious bovine intramammary infections in dairy cattle. Its ability to persist inside the udder is based on the presence of important mechanisms such as its ability to form biofilms, polysaccharide capsules small colony variants, and their ability to invade professiona...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14706
更新日期:2020-11-01 00:00:00