Production and characterization of a new bioemulsifier from Pseudomonas putida ML2.

Abstract:

AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed to determine the emulsifying properties of culture supernatants of P. putida ML2 in defined and complex media. Surface tension of cell-free supernatants was measured. Purification and chemical analysis of the emulsifier was performed. Confirmed results indicate that a polysaccharide with hexasaccharide repeating units is responsible for the emulsifying activity in a mineral medium with glucose as sole carbon source. Moreover, an emulsifier is produced when growing on naphthalene. CONCLUSIONS:Culture media composition influences the amount and the properties of the emulsifier produced by this P. putida strain. Under nitrogen limiting conditions, a polysaccharide is responsible for the emulsifying activity in defined mineral media. In complex nitrogen rich medium, a different kind of emulsifier is produced. The exopolymer may contribute to hydrocarbons solubilization. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first exopolysaccharide with emulsifying properties produced by a Pseudomonas strain reported to the present. Also chemical composition is significantly different from previous reports. This strain has potential use in bioremediation and the purified polysaccharide may be used in food and cosmetic industry. Moreover, the production of the exopolymer may play a role on biofilm formation.

journal_name

J Appl Microbiol

authors

Bonilla M,Olivaro C,Corona M,Vazquez A,Soubes M

doi

10.1111/j.1365-2672.2004.02480.x

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

456-63

issue

2

eissn

1364-5072

issn

1365-2672

pii

JAM2480

journal_volume

98

pub_type

杂志文章
  • Phages of enteric bacteria in fresh water with different levels of faecal pollution.

    abstract::Levels of somatic and F-specific coliphages, and phages infecting Bacteroides fragilis were measured in 257 samples collected in different freshwater environments with different levels and characteristics of faecal pollution. In samples with recent pollution of domestic origin, the numbers of the three groups of phage...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00354.x

    authors: Araujo RM,Puig A,Lasobras J,Lucena F,Jofre J

    更新日期:1997-03-01 00:00:00

  • Thiol redox state and oxidative stress affect sclerotial differentiation of the phytopathogenic fungi Sclerotium rolfsii and Sclerotinia sclerotiorum.

    abstract:AIMS:To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS AND RESULTS:Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic count...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03527.x

    authors: Patsoukis N,Georgiou CD

    更新日期:2008-01-01 00:00:00

  • Inactivation of Escherichia coli by citral.

    abstract:AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04597.x

    authors: Somolinos M,García D,Condón S,Mackey B,Pagán R

    更新日期:2010-06-01 00:00:00

  • Effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen Piscirickettsia salmonis.

    abstract:AIMS:To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. METHODS AND RESULTS:Specific IgY was produced by immunizing hens ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12857

    authors: Oliver C,Valenzuela K,Silva H,Haro RE,Cortés M,Sandoval R,Pontigo JP,Álvarez C,Figueroa JE,Avendaño-Herrera R,Troncoso JM,Yáñez AJ

    更新日期:2015-08-01 00:00:00

  • Impact of pulsed light on cellular activity of Salmonella enterica.

    abstract:AIMS:The objective of this study was a comprehensive characterization of physiological changes of Salmonella enterica induced by intense broad spectrum pulsed light (PL). After exposing the bacteria to this nonthermal decontamination technology on a gel surface, multiple viability parameters beyond culturability were a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13231

    authors: Kramer B,Wunderlich J,Muranyi P

    更新日期:2016-10-01 00:00:00

  • Strain-dependent production of selected bioactive compounds by Cyanobacteria belonging to the Arthrospira genus.

    abstract:AIMS:To investigate the synthesis of selected bioactive compounds in various strains belonging to the Arthrospira genus. METHODS AND RESULTS:The synthesis of vitamin C, polyphenols, vitamin B12, fatty acids and selected volatile compounds (acetone and acetaldehyde) was investigated in six strains belonging to the Arth...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12897

    authors: Satora P,Barwińska-Sendra A,Duda-Chodak A,Wajda Ł

    更新日期:2015-09-01 00:00:00

  • Potential risk-factors affecting Salmonella sp. and Escherichia coli occurrence and distribution in Midwestern United States swine feed mills.

    abstract:AIM:This study aimed to evaluate the patterns and potential risk factors associated with the occurrence of Salmonella sp. and Escherichia coli in selected United States swine feed mills. METHODS AND RESULTS:A total of 405 samples were collected during fall 2018, spring and summer 2019 from selected sites including flo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14758

    authors: Magossi G,Lambertini E,Noll L,Bai J,Jones C,Nagaraja TG,Phebus R,Woodworth J,Trinetta V

    更新日期:2020-12-01 00:00:00

  • A rapid method for the evaluation of both extrinsic and intrinsic contamination and resulting spoilage of water-in-oil emulsions.

    abstract:AIMS:To develop a method for studying the microbial spoilage of water-in-oil emulsions and to use this to investigate (i) the intrinsic stability of water-in-oil formulations and (ii) Pseudomonas aeruginosa SP1-induced spoilage of a proprietary emulsion. METHODS AND RESULTS:Aliquots of test emulsion were placed into w...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02239.x

    authors: O'May GA,Allison DG,Gilbert P

    更新日期:2004-01-01 00:00:00

  • Effects of graded levels of tannin-containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production.

    abstract:AIMS:This study was carried out to determine the effect of graded levels of tannin-containing tropical tree leaves, Autocarpus integrifolis, Azardirachta indica and Ficus bengalensis, on the in vitro rumen fermentation pattern, total protozoa and methane suppression in order to establish the optimum dose of these leave...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12723

    authors: Bhatta R,Saravanan M,Baruah L,Prasad CS

    更新日期:2015-03-01 00:00:00

  • Transmission routes of Salmonella Typhimurium DT 104 between 14 cattle and pig herds in Denmark demonstrated by molecular fingerprinting.

    abstract:AIMS:Salmonella Typhimurium DT 104 is generally assumed to be spread by contact between live animals, e.g. by trading. The aim of the present study was to assess the importance of other routes of transmission in the dissemination of this bacterium. METHODS AND RESULTS:An outbreak among 14 cattle and pig herds located ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02992.x

    authors: Langvad B,Skov MN,Rattenborg E,Olsen JE,Baggesen DL

    更新日期:2006-10-01 00:00:00

  • Aflatoxin contamination of groundnut and maize in Zambia: observed and potential concentrations.

    abstract:AIMS:The aims of the study were to quantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and to determine the vulnerability to aflatoxin increases after purchase. METHODS AND RESULTS:Aflatoxin concentrations were determin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13448

    authors: Kachapulula PW,Akello J,Bandyopadhyay R,Cotty PJ

    更新日期:2017-06-01 00:00:00

  • Effect of biochanin A on corn grain (Zea mays) fermentation by bovine rumen amylolytic bacteria.

    abstract:AIMS:The objective was to determine the effect of biochanin A (BCA), an isoflavone produced by red clover (Trifolium pratense L.), on corn fermentation by rumen micro-organisms. METHODS AND RESULTS:When bovine rumen bacterial cell suspensions (n = 3) were incubated (24 h, 39°C) with ground corn, amylolytic bacteria in...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13397

    authors: Harlow BE,Flythe MD,Aiken GE

    更新日期:2017-04-01 00:00:00

  • Effects of small increases in copper levels on culturable basidiomycetous yeasts in low-nutrient soils.

    abstract:AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04770.x

    authors: Vreulink JM,Stone W,Botha A

    更新日期:2010-10-01 00:00:00

  • Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products.

    abstract:AIMS:Considering the significant rise in the probiotic market in Columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the C...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03294.x

    authors: Perea Vélez M,Hermans K,Verhoeven TL,Lebeer SE,Vanderleyden J,De Keersmaecker SC

    更新日期:2007-09-01 00:00:00

  • Validation of a real-time PCR for Haemophilus parasuis.

    abstract:AIMS:To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis. METHODS AND RESULTS:The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04526.x

    authors: Turni C,Pyke M,Blackall PJ

    更新日期:2010-04-01 00:00:00

  • Metabiosis of proteolytic moulds and Salmonella in raw, ripe tomatoes.

    abstract:AIMS:The aim of this study was to determine the survival and growth characteristics of Salmonella enterica in sound and chill-injured tomatoes as influenced by co-infection with proteolytic moulds. METHODS AND RESULTS:Sound (not chill injured) raw, ripe tomatoes (Lycopersicon esculentum Mill. 'Roma') were inoculated w...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01995.x

    authors: Wade WN,Beuchat LR

    更新日期:2003-01-01 00:00:00

  • Flow cytometry: a versatile technology for specific quantification and viability assessment of micro-organisms in multistrain probiotic products.

    abstract:AIMS:Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but nonculturable (VBNC) micro-organisms and nonviable cells. The aim of the study was to evaluate flow cytometry as a novel me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13666

    authors: Chiron C,Tompkins TA,Burguière P

    更新日期:2018-02-01 00:00:00

  • Novel antibacterial silver-silica surface coatings prepared by chemical vapour deposition for infection control.

    abstract:AIMS:Environmental contamination plays an important role in the transmission of infections, especially healthcare-associated infections. Disinfection transiently reduces contamination, but surfaces can rapidly become re-contaminated. Antimicrobial surfaces may partially overcome that limitation. The antimicrobial activ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12308

    authors: Varghese S,Elfakhri S,Sheel DW,Sheel P,Bolton FJ,Foster HA

    更新日期:2013-11-01 00:00:00

  • Production of laccases by Pleurotus ostreatus in submerged fermentation in co-culture with Trichoderma viride.

    abstract:AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04493.x

    authors: Flores C,Casasanero R,Trejo-Hernández MR,Galindo E,Serrano-Carreón L

    更新日期:2010-03-01 00:00:00

  • Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters.

    abstract:AIMS:To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS:The MPN method was a combination of a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03677.x

    authors: Jenkins MB,Endale DM,Fisher DS

    更新日期:2008-06-01 00:00:00

  • Antigen presenting and effector cell cluster formation in BALB/c mice during mousepox: model studies*.

    abstract:AIMS:The objective of this study was to access APC-effector cell cluster formation in genetically susceptible BALB/c (H-2(d) ) mice infected with highly virulent Moscow strain of ectromelia virus (ECTV-MOS) and estimate of lymphocyte activation based upon expression of CD62L and CD44 molecules. METHODS AND RESULTS:APC...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04813.x

    authors: Szulc L,Boratynska A,Martyniszyn L,Niemialtowski MG

    更新日期:2010-11-01 00:00:00

  • In vitro evaluation of metal chelators as potential metallo- β -lactamase inhibitors.

    abstract:AIMS:This study aimed at investigating the use of metal chelators as potential metallo-β-lactamase inhibitors (MBL). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of meropenem was ascertained alone and in combination with various concentrations of macrocyclic (1,4,7- triazacyclononane-1-glutaric acid-...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13085

    authors: Azumah R,Dutta J,Somboro AM,Ramtahal M,Chonco L,Parboosing R,Bester LA,Kruger HG,Naicker T,Essack SY,Govender T

    更新日期:2016-04-01 00:00:00

  • Influence of phenothiazines, phenazines and phenoxazine on cation transport in Candida albicans.

    abstract:AIMS:(i) To analyse the increase in calcium ion uptake caused by several cationic dyes on Candida albicans, (ii) to postulate a mechanism, (iii) to define the effects of Zn ions on the phenomenon, and (iv) to propose the use of the dyes or their derivatives against C. albicans. METHODS AND RESULTS:Cells were grown in ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14092

    authors: Calahorra M,Sánchez NS,Peña A

    更新日期:2018-08-28 00:00:00

  • Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica.

    abstract::A number of phenotypic and molecular fingerprinting techniques, including physiological profiling (Biolog), restriction fragment length polymorphism (RFLP), enterobacterial repetitive intergenic consensus (ERIC) and a phage typing system, were evaluated for their ability to differentiate between 60 strains of Erwinia ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00929.x

    authors: Toth IK,Bertheau Y,Hyman LJ,Laplaze L,López MM,McNicol J,Niepold F,Persson P,Salmond GP,Sletten A,van Der Wolf JM,Pérombelon MC

    更新日期:1999-11-01 00:00:00

  • Distribution and survival of Vibrio vulnificus genotypes in postharvest Gulf Coast (USA) oysters under refrigeration.

    abstract:AIMS:  The effect of refrigeration on the seafood-borne pathogen Vibrio vulnificus was investigated in terms of genotype selection and persistence in refrigerated oysters. METHODS AND RESULTS:  Naturally occurring numbers of V. vulnificus in oysters from two different locations were compared during a 2-week period und...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05306.x

    authors: Wood RR,Arias CR

    更新日期:2012-07-01 00:00:00

  • Dynamics of living and dead bacterial cells within a mixed-species biofilm during toluene degradation in a biotrickling filter.

    abstract:AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01914.x

    authors: Tresse O,Lescob S,Rho D

    更新日期:2003-01-01 00:00:00

  • Beneficial effects of probiotics in upper respiratory tract infections and their mechanical actions to antagonize pathogens.

    abstract::Probiotics are live micro-organisms with beneficial effects on human health, which have the ability to counteract infections at different locations of the body. Clinical trials have shown that probiotics can be used as preventive and therapeutic agents in upper respiratory tract infections (URTIs) and otitis. Their me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2012.05394.x

    authors: Popova M,Molimard P,Courau S,Crociani J,Dufour C,Le Vacon F,Carton T

    更新日期:2012-12-01 00:00:00

  • Common occurrence of Gallid herpesvirus-2 with reticuloendotheliosis virus in chickens caused by possible contamination of vaccine stocks.

    abstract:AIMS:The aim of this study was to investigate the common occurrence of reticuloendotheliosis virus (REV) among Gallid herpesvirus 2 (GaHV-2) infected chickens. The possible cause of this co-occurrence may be linked to contaminated vaccine stocks, which were also examined. METHODS AND RESULTS:The study was conducted on...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12734

    authors: Woźniakowski G,Mamczur A,Samorek-Salamonowicz E

    更新日期:2015-04-01 00:00:00

  • Thermal inactivation of Listeria monocytogenes and Yersinia enterocolitica in minced beef under laboratory conditions and in sous-vide prepared minced and solid beef cooked in a commercial retort.

    abstract::D-values were obtained for Listeria monocytogenes and Yersinia enterocolitica at 50, 55 and 60 degrees C in vacuum-packed minced beef samples heated in a laboratory water-bath. The experiment was repeated using vacutainers, which allowed heating of the beef to the desired temperature before inoculation. D-values of be...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01001.x

    authors: Bolton DJ,McMahon CM,Doherty AM,Sheridan JJ,McDowell DA,Blair IS,Harrington D

    更新日期:2000-04-01 00:00:00

  • Global gene expression of Trichophyton rubrum in response to PH11B, a novel fatty acid synthase inhibitor.

    abstract:AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03521.x

    authors: Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

    更新日期:2007-12-01 00:00:00