Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica.

abstract：AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find th...

journal_title：Journal of applied microbiology

authors： Lin J,Yan XJ,Zheng L,Ma HH,Chen HM

更新日期：2005-01-01 00:00:00

abstract：:The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...

journal_title：Journal of applied microbiology

authors： Wang Y,McAllister TA,Yanke LJ,Cheeke PR

更新日期：2000-05-01 00:00:00

abstract：AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...

journal_title：Journal of applied microbiology

authors： Bravo-Ferrada BM,Gonçalves S,Semorile L,Santos NC,Tymczyszyn EE,Hollmann A

更新日期：2015-06-01 00:00:00

abstract：AIMS:This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (LAB) isolated from sugarcane grown in a Brazil on the quality of corn silage. METHODS AND RESULTS:Three strains of Lactobacillus buchneri (UFLA SLM11, UFLA SLM103 and UFLA SLM108), five strains of Lactobacillus plantarum (...

journal_title：Journal of applied microbiology

authors： Santos AO,Ávila CL,Pinto JC,Carvalho BF,Dias DR,Schwan RF

更新日期：2016-02-01 00:00:00

abstract：AIMS:To characterize the non-spoilage variant obtained from beer-spoilage Lactobacillus brevis ABBC45C and to identify a potential genetic marker capable of discriminating beer-spoilage L. brevis strains from non-spoilers. METHODS AND RESULTS:A non-spoilage variant was obtained from beer-spoilage L. brevis ABBC45C by ...

journal_title：Journal of applied microbiology

authors： Suzuki K,Koyanagi M,Yamashita H

更新日期：2004-01-01 00:00:00

abstract：AIMS:To investigate the influence of micro-organisms associated with copper corrosion on 'blue water' corrosion in drinking water. METHODS AND RESULTS:Laboratory rigs comprising of polycarbonate containers attached to annealed copper plumbing tubes were filled with Melbourne drinking water and sterilized by autoclavin...

journal_title：Journal of applied microbiology

authors： Critchley MM,Pasetto R,O'Halloran RJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...

journal_title：Journal of applied microbiology

authors： Cenci G,Trotta F,Caldini G

更新日期：2006-12-01 00:00:00

abstract：AIMS:To determine the capacity of extracts of Pelargonium reniforme and Pelargonium sidoides, plants of the Geraniaceae family, to stimulate the uptake and killing of mycobacteria by murine macrophages and to identify the constituents that are responsible. METHODS AND RESULTS:Bioassay-guided fractionation of aqueous P...

journal_title：Journal of applied microbiology

authors： Kim CE,Griffiths WJ,Taylor PW

更新日期：2009-04-01 00:00:00

abstract：AIMS:To characterize the two-component cell lysis cassette comprised of holin (Hyb5) and endolysin (Lyb5) encoded by Lactobacillus fermentum temperate bacteriophage phiPYB5, and illustrate the potential application of Lyb5 as therapeutic agents. METHODS AND RESULTS:The hyb5-lyb5 cassette was cloned from the genome lib...

journal_title：Journal of applied microbiology

authors： Wang S,Kong J,Zhang X

更新日期：2008-12-01 00:00:00

abstract：:Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes fr...

journal_title：Journal of applied microbiology

authors： Son R,Rusu G,Karim MI

更新日期：1997-02-01 00:00:00

abstract：AIMS:To identify bacterial pathogens of diseased NiIe tilapia Oreochromis niloticus and determine their virulence. METHODS AND RESULTS:Sixteen bacterial isolates were recovered from diseased Nile tilapias (O. niloticus) reared in floating cages in Adolfo Lopez Mateos (ALM), Sanalona and Dique IV dams in Sinaloa, Mexic...

journal_title：Journal of applied microbiology

authors： Soto-Rodriguez SA,Cabanillas-Ramos J,Alcaraz U,Gomez-Gil B,Romalde JL

更新日期：2013-09-01 00:00:00

abstract：AIMS:The ability of 31 Lactobacillus plantarum strains to adhere to biological matrixes was evaluated, and the molecules involved in adherence were studied. METHODS AND RESULTS:Mucin, basement membrane proteins and Caco-2 cells were used in adhesion tests. These in vitro assays, together with a yeast agglutination tes...

journal_title：Journal of applied microbiology

authors： Tallon R,Arias S,Bressollier P,Urdaci MC

更新日期：2007-02-01 00:00:00

abstract：AIMS:To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. METHODS AND RESULTS:Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragm...

journal_title：Journal of applied microbiology

authors： Arias CR,Welker TL,Shoemaker CA,Abernathy JW,Klesius PH

更新日期：2004-01-01 00:00:00

abstract：AIMS:We aimed at expressing heterologous proteins in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees, as a prerequisite for future studies on the molecular pathogenesis of P. larvae infections. METHODS AND RESULTS:For this purpose, we established a protocol for the transformation of the p...

journal_title：Journal of applied microbiology

authors： Poppinga L,Genersch E

更新日期：2012-03-01 00:00:00

abstract：AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...

journal_title：Journal of applied microbiology

authors： Baudoin E,Couillerot O,Spaepen S,Moënne-Loccoz Y,Nazaret S

更新日期：2010-01-01 00:00:00

abstract：AIM:To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS:The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated...

journal_title：Journal of applied microbiology

authors： Ercan UK,Sen B,Brooks AD,Joshi SG

更新日期：2018-08-01 00:00:00

abstract：AIMS:Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm m...

journal_title：Journal of applied microbiology

authors： Rudney JD,Chen R,Lenton P,Li J,Li Y,Jones RS,Reilly C,Fok AS,Aparicio C

更新日期：2012-12-01 00:00:00

abstract：AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatograp...

journal_title：Journal of applied microbiology

authors： Liu M,Wang H,Griffiths MW

更新日期：2007-12-01 00:00:00

abstract：AIMS:Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt st...

journal_title：Journal of applied microbiology

authors： Irizarry I,White JF

更新日期：2017-04-01 00:00:00

abstract：AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...

journal_title：Journal of applied microbiology

authors： Burton JP,Chilcott CN,Moore CJ,Speiser G,Tagg JR

更新日期：2006-04-01 00:00:00

abstract：AIMS:The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan equine encephalitis virus (VEEV), on two material types. METHODS AND RESULTS:Francisella tularensis (F.t.) and VEEV were inoculated (c. 1 × 108 colony-form...

journal_title：Journal of applied microbiology

authors： Richter WR,Sunderman MM,Wendling MQS,Serre S,Mickelsen L,Rupert R,Wood J,Choi Y,Willenberg Z,Calfee MW

更新日期：2020-04-01 00:00:00

abstract：AIMS:To monitor emissions of NH(3) and N(2)O during composting and link these to ammonia oxidation rates and the community structure of ammonia oxidizing bacteria (AOB). METHODS AND RESULTS:A laboratory-scale compost reactor treating organic household waste was run for 2 months. NH(3) emissions peaked when pH started ...

journal_title：Journal of applied microbiology

authors： Jarvis A,Sundberg C,Milenkovski S,Pell M,Smårs S,Lindgren PE,Hallin S

更新日期：2009-05-01 00:00:00

abstract：AIMS:Probiotics have the ability to enhance the immune system, produce anti-inflammatory action and promote wound healing process. The first aim of the study was to isolate pathogenic micro-organisms from sites of chronic ulcerative lesion. The second aim was to evaluate probiotic efficacy of SYNBIO® (1:1 combination o...

journal_title：Journal of applied microbiology

authors： Coman MM,Mazzotti L,Silvi S,Scalise A,Orpianesi C,Cresci A,Verdenelli MC

更新日期：2020-02-01 00:00:00

abstract：:Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...

journal_title：Journal of applied microbiology

authors： Margolles A,Mayo B,de los Reyes-Gavilán CG

更新日期：1998-02-01 00:00:00

abstract：AIMS:To assess the biodiversity of a large number of microbial fuel cell (MFC) anodes from a variety of MFC designs, all enriched with domestic wastewater, using a molecular fingerprinting method. METHODS AND RESULTS:We optimized a protocol allowing the rapid characterization of MFC communities using terminal restrict...

journal_title：Journal of applied microbiology

authors： Lefebvre O,Nguyen TT,Al-Mamun A,Chang IS,Ng HY

更新日期：2010-09-01 00:00:00

abstract：AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...

journal_title：Journal of applied microbiology

authors： Bravo SME,Morales M,Del Mónaco SM,Caballero AC

更新日期：2019-05-01 00:00:00

abstract：AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...

journal_title：Journal of applied microbiology

authors： Kim SY,Shin SJ,Song CH,Jo EK,Kim HJ,Park JK

更新日期：2009-03-01 00:00:00

abstract：AIMS:The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. METHODS AND RESULTS:Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological proce...

journal_title：Journal of applied microbiology

authors： Gomes RC,Sêmedo LT,Soares RM,Linhares LF,Ulhoa CJ,Alviano CS,Coelho RR

更新日期：2001-04-01 00:00:00

abstract：AIMS:This study investigates changes in microbiological and physicochemical parameters during large-scale, thermophilic composting of a single batch of municipal organic waste. The inter-relationships between the microbial biomass and community structure as well as several physicochemical parameters and estimates of ma...

journal_title：Journal of applied microbiology

authors： Steger K,Sjögren AM,Jarvis A,Jansson JK,Sundh I

更新日期：2007-08-01 00:00:00

abstract：AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...

journal_title：Journal of applied microbiology

authors： Bourriaud C,Robins RJ,Martin L,Kozlowski F,Tenailleau E,Cherbut C,Michel C

更新日期：2005-01-01 00:00:00