Abstract:
AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. CONCLUSIONS:In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. SIGNIFICANCE AND IMPACT OF THE STUDY:Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Bravo-Ferrada BM,Gonçalves S,Semorile L,Santos NC,Tymczyszyn EE,Hollmann Adoi
10.1111/jam.12796subject
Has Abstractpub_date
2015-06-01 00:00:00pages
1409-17issue
6eissn
1364-5072issn
1365-2672journal_volume
118pub_type
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