Identification and virulence of Aeromonas dhakensis, Pseudomonas mosselii and Microbacterium paraoxydans isolated from Nile tilapia, Oreochromis niloticus, cultivated in Mexico.

Abstract:

AIMS:To identify bacterial pathogens of diseased NiIe tilapia Oreochromis niloticus and determine their virulence. METHODS AND RESULTS:Sixteen bacterial isolates were recovered from diseased Nile tilapias (O. niloticus) reared in floating cages in Adolfo Lopez Mateos (ALM), Sanalona and Dique IV dams in Sinaloa, Mexico, from February to May 2009. The bacterial isolates were identified by phenotypic and molecular (rep-PCR and 16S rRNA sequencing) methods and were mostly isolated from the kidneys and the brain of tilapias. Bacterial cells and extracellular products (ECPs) of strains were characterized and used in experimental infections with sole Solea vulgaris and Mozambican tilapia Oreochromis mossambicus. The fish challenged with Aeromonas dhakensis sp. nov. comb nov, Pseudomonas mosselii and Microbacterium paraoxydans (3·1 × 10(6)  CFU g(-) 1) exhibited mortality between 40 and 100% starting at 6 h postinoculation. The ECPs displayed gelatinase, haemolytic and cytotoxic activity, causing the total destruction of the HeLa cell lines. CONCLUSIONS:Aeromonas dhakensis and Ps. mosselii were virulent to O. mossambicus, whereas Mic. paraoxydans displayed virulence to S. vulgaris. SIGNIFICANCE AND IMPACT OF THE STUDY:This the first time that Aeromonas dhakensis and Ps. mosselii are reported as pathogens to tilapia and Mic. paraoxydans was isolated from fish; then, these fish pathogens could be a threat to farmed Nile tilapia in Mexico.

journal_name

J Appl Microbiol

authors

Soto-Rodriguez SA,Cabanillas-Ramos J,Alcaraz U,Gomez-Gil B,Romalde JL

doi

10.1111/jam.12280

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

654-62

issue

3

eissn

1364-5072

issn

1365-2672

journal_volume

115

pub_type

杂志文章
  • Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease.

    abstract:AIM:To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease. METHODS AND RESULTS:Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and fiel...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03611.x

    authors: Haggag WM,Timmusk S

    更新日期:2008-04-01 00:00:00

  • Role of botrytized grape micro-organisms in SO2 binding phenomena.

    abstract:AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01200.x

    authors: Barbe JC,De Revel G,Joyeux A,Bertrand A,Lonvaud-Funel A

    更新日期:2001-01-01 00:00:00

  • Essential oils inhibit the bovine respiratory pathogens Mannheimia haemolytica, Pasteurella multocida and Histophilus somni and have limited effects on commensal bacteria and turbinate cells in vitro.

    abstract:AIMS:The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS:The chemical composition of 16 EOs was determ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14238

    authors: Amat S,Baines D,Timsit E,Hallewell J,Alexander TW

    更新日期:2019-06-01 00:00:00

  • Luffa sponge offsets the negative effects of aeration on bacterial cellulose production.

    abstract:AIMS:To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS:The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13290

    authors: Krusong W,Kerdpiboon S,Pornpukdeewattana S,Jindaprasert A

    更新日期:2016-12-01 00:00:00

  • Phenotypic and genotypic characterization of competitive exclusion products for use in poultry.

    abstract:AIMS:Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products. METHODS AND RESULTS:Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utili...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01944.x

    authors: Wagner RD,Paine DD,Cerniglia CE

    更新日期:2003-01-01 00:00:00

  • Impact of pulsed light on cellular activity of Salmonella enterica.

    abstract:AIMS:The objective of this study was a comprehensive characterization of physiological changes of Salmonella enterica induced by intense broad spectrum pulsed light (PL). After exposing the bacteria to this nonthermal decontamination technology on a gel surface, multiple viability parameters beyond culturability were a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13231

    authors: Kramer B,Wunderlich J,Muranyi P

    更新日期:2016-10-01 00:00:00

  • The prevalence of antimicrobial-resistant Escherichia coli in sympatric wild rodents varies by season and host.

    abstract:AIMS:  To investigate the prevalence and temporal patterns of antimicrobial resistance in wild rodents with no apparent exposure to antimicrobials. METHODS AND RESULTS:  Two sympatric populations of bank voles and wood mice were trapped and individually monitored over a 2- year period for faecal carriage of antimicrob...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04952.x

    authors: Williams NJ,Sherlock C,Jones TR,Clough HE,Telfer SE,Begon M,French N,Hart CA,Bennett M

    更新日期:2011-04-01 00:00:00

  • Flavanols protect the yeast Saccharomyces cerevisiae against heating and freezing/thawing injury.

    abstract:AIMS:The aim of the study was to check whether two flavanols ((-)-epigallocatechin gallate and (+)-catechin) can ameliorate oxidative stress (OS) accompanying and contributing to the lethal effects of heating (50°C) and freezing-thawing on the yeast Saccharomyces cerevisiae. METHODS AND RESULTS:The flavanols studied i...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14170

    authors: Naparlo K,Zyracka E,Bartosz G,Sadowska-Bartosz I

    更新日期:2019-03-01 00:00:00

  • Global gene expression of Trichophyton rubrum in response to PH11B, a novel fatty acid synthase inhibitor.

    abstract:AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03521.x

    authors: Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

    更新日期:2007-12-01 00:00:00

  • Rapid in vivo screening system for anti-oxidant activity using bacterial redox sensor strains.

    abstract:AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04514.x

    authors: Park SJ,Chung HY,Lee JH

    更新日期:2010-04-01 00:00:00

  • Lactobacillus helveticus heterogeneity in natural cheese starters: the diversity in phenotypic characteristics.

    abstract::The study of wild strains from natural habitats is a useful means of understanding better the heterogeneity within a species of biotechnological importance, and of obtaining atypical isolates with unknown capabilities. In the present research carried out on different Lactobacillus helveticus strains isolated from natu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00312.x

    authors: Fortina MG,Nicastro G,Carminati D,Neviani E,Manachini PL

    更新日期:1998-01-01 00:00:00

  • Ochratoxin A removal in synthetic and natural grape juices by selected oenological Saccharomyces strains.

    abstract:AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02385.x

    authors: Bejaoui H,Mathieu F,Taillandier P,Lebrihi A

    更新日期:2004-01-01 00:00:00

  • Characterization of the germination of Bacillus megaterium spores lacking enzymes that degrade the spore cortex.

    abstract:AIMS:To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS:Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04210.x

    authors: Setlow B,Peng L,Loshon CA,Li YQ,Christie G,Setlow P

    更新日期:2009-07-01 00:00:00

  • A preliminary study of the effect of probiotic Streptococcus salivarius K12 on oral malodour parameters.

    abstract:AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02837.x

    authors: Burton JP,Chilcott CN,Moore CJ,Speiser G,Tagg JR

    更新日期:2006-04-01 00:00:00

  • Microbiological quality and safety of minimally processed parsley (Petroselinum crispum) sold in food markets, southeastern Brazil.

    abstract:AIMS:This study evaluated the microbiological quality and safety of minimally processed parsley sold in southeastern Brazilian food markets. METHODS AND RESULTS:One hundred samples were submitted to the enumeration of Enterobacteriaceae by plating on MacConkey agar. Colonies of Enterobacteriaceae were randomly selecte...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14935

    authors: Finger JAFF,Maffei DF,Dias M,Mendes MA,Pinto UM

    更新日期:2020-11-19 00:00:00

  • On the challenges of detecting whole Staphylococcus aureus cells with biosensors.

    abstract::Due to the increasing number of nosocomial infections and multidrug-resistant bacterial strains, Staphylococcus aureus is now a major worldwide concern. Rapid detection and characterization of this bacterium has become an important issue for biomedical applications. Biosensors are increasingly appearing as low-cost, e...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13510

    authors: Templier V,Roupioz Y

    更新日期:2017-11-01 00:00:00

  • Adaptive changes in geranylgeranyl pyrophosphate synthase gene expression level under ethanol stress conditions in Oenococcus oeni.

    abstract:AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12351

    authors: Cafaro C,Bonomo MG,Salzano G

    更新日期:2014-01-01 00:00:00

  • An eight-month study of a population of verocytotoxigenic Escherichia coli (VTEC) in a Scottish cattle herd.

    abstract:AIMS:Strains of Verocytotoxin-producing Escherichia coli (VTEC) from Scottish beef cattle on the same farm were isolated during four visits over a period of eight months. Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection. METHODS AND RESULTS:Strain...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01771.x

    authors: Jenkins C,Pearce MC,Chart H,Cheasty T,Willshaw GA,Gunn GJ,Dougan G,Smith HR,Synge BA,Frankel G

    更新日期:2002-01-01 00:00:00

  • Isolation of lytic bacteriophage against Vibrio harveyi.

    abstract:AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04578.x

    authors: Crothers-Stomps C,Høj L,Bourne DG,Hall MR,Owens L

    更新日期:2010-05-01 00:00:00

  • The response of Leuconostoc mesenteroides to low external oxidoreduction potential generated by hydrogen gas.

    abstract:AIMS:The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated. METHODS AND RESULTS:Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions (Eh7: +200 mV and -400 mV) using nitrogen and hydrogen as reducing agents. Growth wa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01832.x

    authors: Bourel G,Henini S,Diviès C,Garmyn D

    更新日期:2003-01-01 00:00:00

  • Multiple competitive PCR-DGGE as a tool for quantifying and profiling defined mixed cultures of lactic acid bacteria during production of probiotics from complex polysaccharides.

    abstract:AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.02058.x

    authors: Pintado J,Guyot JP,Ampe F

    更新日期:2003-01-01 00:00:00

  • Taxonomic and functional diversity of atrazine-degrading bacterial communities enriched from agrochemical factory soil.

    abstract:AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04700.x

    authors: Udiković-Kolić N,Hršak D,Devers M,Klepac-Ceraj V,Petrić I,Martin-Laurent F

    更新日期:2010-07-01 00:00:00

  • WarmStart colorimetric RT-LAMP for the rapid, sensitive and specific detection of Enteroviruses A-D targeting the 5'UTR region.

    abstract:AIMS:The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species A-D targeting the 5' untranslated region (5' UTR) of enteroviruses genome. METHODS AND RESULTS:The RNA was converted to cDNA by the reverse transcriptase and then amplified via LAMP by the...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14770

    authors: Daskou M,Dimitriou TG,Alexopoulou DS,Tsakogiannis D,Amoutzias GD,Mossialos D,Kyriakopoulou Z,Markoulatos P

    更新日期:2021-01-01 00:00:00

  • Evaluation of autochthonous micrococcus strains as starter cultures for the production of Kedong sufu.

    abstract:AIMS:The technological properties of 22 micrococcus strains from traditional fermented Kedong sufu were evaluated in order to develop autochthonous starter cultures. METHODS AND RESULTS:The proteolytic, autolytic and lipolytic activity, salt tolerance, production and degradation of the biogenic amines of six Micrococc...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13023

    authors: Feng Z,Huang S,Ai ZW,Zhang M,Zhai S,Chen X

    更新日期:2016-03-01 00:00:00

  • Solar disinfection of drinking water contained in transparent plastic bottles: characterizing the bacterial inactivation process.

    abstract::A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00455.x

    authors: McGuigan KG,Joyce TM,Conroy RM,Gillespie JB,Elmore-Meegan M

    更新日期:1998-06-01 00:00:00

  • Effects of sublethal doses of silver nanoparticles on Bacillus subtilis planktonic and sessile cells.

    abstract:AIMS:Due to their antimicrobial activity, silver nanoparticles (Ag-NPs) are being increasingly used in a number of industrial products. The accumulation of Ag-NPs in the soil might affect plant growth-promoting rhizobacteria and, in turn, the plants. We describe the effects of Ag-NPs on the soil bacteria Azotobacter vi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12779

    authors: Gambino M,Marzano V,Villa F,Vitali A,Vannini C,Landini P,Cappitelli F

    更新日期:2015-05-01 00:00:00

  • Drought degree constrains the beneficial effects of a fungal endophyte on Atractylodes lancea.

    abstract:AIMS:Plants, fungal endophytes (FEs) and the changing environment interact with each other forming an interlaced network. This study evaluates nonadditive and interactive effects of the FE Acremonium strictum and drought treatment on Atractylodes lancea plantlets. METHODS AND RESULTS:By applying FEs (meristem cultures...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12615

    authors: Yang T,Ma S,Dai CC

    更新日期:2014-11-01 00:00:00

  • Biotechnological application of yeasts in food science: Starter cultures, probiotics and enzyme production.

    abstract::This review is an account of experiences of two research teams (from Italy to Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. This review covers th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13548

    authors: Arevalo-Villena M,Briones-Perez A,Corbo MR,Sinigaglia M,Bevilacqua A

    更新日期:2017-12-01 00:00:00

  • Destruction of Bacillus licheniformis spores by microwave irradiation.

    abstract:AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04056.x

    authors: Kim SY,Shin SJ,Song CH,Jo EK,Kim HJ,Park JK

    更新日期:2009-03-01 00:00:00

  • Bacterial community dynamics and product distribution during pH-adjusted fermentation of vegetable wastes.

    abstract:AIMS:To estimate the effect of pH on the structures of bacterial community during fermentation of vegetable wastes and to investigate the relationship between bacterial community dynamics and product distribution. METHODS AND RESULTS:The bacterial communities in five batch tests controlled at different pH values [unco...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03321.x

    authors: Ye NF,Lü F,Shao LM,Godon JJ,He PJ

    更新日期:2007-10-01 00:00:00