Abstract:
AIMS:To identify bacterial pathogens of diseased NiIe tilapia Oreochromis niloticus and determine their virulence. METHODS AND RESULTS:Sixteen bacterial isolates were recovered from diseased Nile tilapias (O. niloticus) reared in floating cages in Adolfo Lopez Mateos (ALM), Sanalona and Dique IV dams in Sinaloa, Mexico, from February to May 2009. The bacterial isolates were identified by phenotypic and molecular (rep-PCR and 16S rRNA sequencing) methods and were mostly isolated from the kidneys and the brain of tilapias. Bacterial cells and extracellular products (ECPs) of strains were characterized and used in experimental infections with sole Solea vulgaris and Mozambican tilapia Oreochromis mossambicus. The fish challenged with Aeromonas dhakensis sp. nov. comb nov, Pseudomonas mosselii and Microbacterium paraoxydans (3·1 × 10(6) CFU g(-) 1) exhibited mortality between 40 and 100% starting at 6 h postinoculation. The ECPs displayed gelatinase, haemolytic and cytotoxic activity, causing the total destruction of the HeLa cell lines. CONCLUSIONS:Aeromonas dhakensis and Ps. mosselii were virulent to O. mossambicus, whereas Mic. paraoxydans displayed virulence to S. vulgaris. SIGNIFICANCE AND IMPACT OF THE STUDY:This the first time that Aeromonas dhakensis and Ps. mosselii are reported as pathogens to tilapia and Mic. paraoxydans was isolated from fish; then, these fish pathogens could be a threat to farmed Nile tilapia in Mexico.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Soto-Rodriguez SA,Cabanillas-Ramos J,Alcaraz U,Gomez-Gil B,Romalde JLdoi
10.1111/jam.12280subject
Has Abstractpub_date
2013-09-01 00:00:00pages
654-62issue
3eissn
1364-5072issn
1365-2672journal_volume
115pub_type
杂志文章abstract:AIM:To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease. METHODS AND RESULTS:Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and fiel...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02385.x
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04210.x
更新日期:2009-07-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.02837.x
更新日期:2006-04-01 00:00:00
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doi:10.1046/j.1365-2672.2002.01771.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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