Phenotypic and genotypic characterization of competitive exclusion products for use in poultry.

Abstract:

AIMS:Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products. METHODS AND RESULTS:Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utilization profiles. Genotypic methods were MicroSeq16S rRNA sequence analysis and BLAST searches of the GenBank sequence database. Agreement between phenotypic and genotypic methods for identification of bacteria isolated from the Preempt CE product was 20%. A defined test mixture of bacteria was identified to the species level 100% by BLAST analysis, 64% by MicroSeq and 36% by phenotypic techniques. CONCLUSIONS:The wide range of facultative and obligate anaerobic bacteria present in a CE product are more accurately identified with 16S rRNA sequence analyses than with phenotypic identification techniques. SIGNIFICANCE AND IMPACT OF THE STUDY:These results will provide guidelines for manufacturers of CE products to submit more reliable product information for market approval by regulatory agencies.

journal_name

J Appl Microbiol

authors

Wagner RD,Paine DD,Cerniglia CE

doi

10.1046/j.1365-2672.2003.01944.x

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

1098-107

issue

6

eissn

1364-5072

issn

1365-2672

pii

1944

journal_volume

94

pub_type

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