Abstract:
AIMS:Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products. METHODS AND RESULTS:Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utilization profiles. Genotypic methods were MicroSeq16S rRNA sequence analysis and BLAST searches of the GenBank sequence database. Agreement between phenotypic and genotypic methods for identification of bacteria isolated from the Preempt CE product was 20%. A defined test mixture of bacteria was identified to the species level 100% by BLAST analysis, 64% by MicroSeq and 36% by phenotypic techniques. CONCLUSIONS:The wide range of facultative and obligate anaerobic bacteria present in a CE product are more accurately identified with 16S rRNA sequence analyses than with phenotypic identification techniques. SIGNIFICANCE AND IMPACT OF THE STUDY:These results will provide guidelines for manufacturers of CE products to submit more reliable product information for market approval by regulatory agencies.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Wagner RD,Paine DD,Cerniglia CEdoi
10.1046/j.1365-2672.2003.01944.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
1098-107issue
6eissn
1364-5072issn
1365-2672pii
1944journal_volume
94pub_type
杂志文章abstract:AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02385.x
更新日期:2004-01-01 00:00:00
abstract:AIM:To determine the sensitivity of methods for detection of injured and uninjured Escherichia coli O157:H7 (E. coli O157) in raw and pasteurized milk. METHODS AND RESULTS:Raw milk, pasteurized milk with 1.5% fat content and pasteurized milk with 3.5% fat content were spiked with E. coli O157 at low levels. The sample...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01646.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.03042.x
更新日期:2006-12-01 00:00:00
abstract:AIMS:The aims of this study were to: (1) estimate the effectiveness of ultraviolet radiation (UV) and sulfuric-acid based fertilizer (SA), at reducing levels of generic Escherichia coli in surface irrigation water and on produce and surface soil in open produce fields; and (2) describe the population dynamics of generi...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.15011
更新日期:2021-01-22 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/jam.14238
更新日期:2019-06-01 00:00:00
abstract:AIMS:To characterize airborne virus-like particles isolated from two cheese production plants in order to reveal their complexity in terms of viral communities and microbial genes potentially mobilized by viruses. METHODS AND RESULTS:Airborne virus-like particles have been isolated from Grana Padano and Gorgonzola PDO...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.14046
更新日期:2018-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13250
更新日期:2016-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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更新日期:2017-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14408
更新日期:2019-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12576
更新日期:2014-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02244.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14474
更新日期:2020-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14990
更新日期:2021-01-07 00:00:00
abstract:AIM:To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS:Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying pla...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01768.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated. METHODS AND RESULTS:Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions (Eh7: +200 mV and -400 mV) using nitrogen and hydrogen as reducing agents. Growth wa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01832.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To identify the chemical structure of a bacteriocin, thermophilin 1277, produced by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277 was purified and partial N-terminal sequence analysis revealed 6 unidentified amino acids amongst 31 amino acids residues. A 2.7-kbp region containing the t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04059.x
更新日期:2009-03-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2002.01535.x
更新日期:2002-01-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2001.01400.x
更新日期:2001-08-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01547.x
更新日期:2002-01-01 00:00:00
abstract::Members of the genus Nocardiopsis are an ecologically versatile and biotechnologically important group of Actinomycetes. Most of the isolates are halotolerant or halophilic and they prevail in soils, marine environments or hypersaline locations. To aid their survival under these conditions, they mainly produce extremo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.12950
更新日期:2016-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04241.x
更新日期:2009-08-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03607.x
更新日期:2008-03-01 00:00:00
abstract::The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00770.x
更新日期:1999-05-01 00:00:00
abstract::During the first half of the century, the majority of anthrax outbreaks in Canada occurred in the southern portions of Ontario and Quebec and were often associated with pastures contaminated by effluent from textile industries dealing with imported animal materials. In 1952, introduction of Federal regulations requiri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2672.1999.00872.x
更新日期:1999-08-01 00:00:00
abstract:AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02837.x
更新日期:2006-04-01 00:00:00
abstract:AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12796
更新日期:2015-06-01 00:00:00
abstract:AIMS:To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS:The ApcMin/+ mouse model was used to study the potential preventive effect of LGG on intestinal poly...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13386
更新日期:2017-03-01 00:00:00
abstract:AIMS:The aim of this study was to investigate changes in Salmonella and total viable count (TVC) survival on beef carcass surfaces stored for 72 h under different combinations of relative humidity (i.e. RH 75% or 96%) and temperature (5 degrees C or 10 degrees C). METHODS AND RESULTS:The influence of low water activit...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03989.x
更新日期:2009-01-01 00:00:00