Methicillin resistance study in clinical isolates of coagulase-negative staphylococci and determination of their susceptibility to alternative antimicrobial agents.

Abstract:

AIMS:To achieve reliable detection of methicillin resistance in clinical isolates of coagulase-negative staphylococci. METHODS AND RESULTS:Strains (105) were evaluated by normatized antimicrobial susceptibility methods, and for the presence of the methicillin resistance-determining mecA gene, using the polymerase chain reaction. Correlation between phenotypic and genotypic methods was obtained in 87.6% of the samples. Six strains, classified as methicillin-susceptible by phenotypic assays, revealed the presence of the mecA gene, indicating that methicillin resistance expression was probably repressed. Another seven isolates failed to show mecA amplification after displaying methicillin resistance in phenotypic evaluations. The susceptibility of the methicillin-resistant isolates to other antimicrobial agents was variable. CONCLUSION:Genotypic determination of the mecA gene proved to be the most reliable method for detection of methicillin resistance. SIGNIFICANCE AND IMPACT OF THE STUDY:Correct assessment of methicillin resistance, such as that attained through genotyping, is essential for defining therapeutic strategies, particularly when treating severely compromised patients.

journal_name

J Appl Microbiol

authors

Bogado I,Sutich E,Krapp A,Marchiaro P,Marzi M,Putero J,Carrillo N

doi

10.1046/j.1365-2672.2001.01400.x

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

344-50

issue

2

eissn

1364-5072

issn

1365-2672

pii

jam1400

journal_volume

91

pub_type

杂志文章
  • Microbiological monitoring in the biodegradation of sewage sludge and food waste.

    abstract:AIM:To study the microbiology of intensive, in-vessel biodegradation of a mixture of sewage sludge and vegetable food waste. METHODS AND RESULTS:The biodegradation was performed in a closed reactor with the addition of a starter culture of Bacillus thermoamylovorans SW25 under conditions of controlled aeration, stirri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02182.x

    authors: Ivanov VN,Wang JY,Stabnikova OV,Tay ST,Tay JH

    更新日期:2004-01-01 00:00:00

  • Novel targets for the future development of antibacterial agents.

    abstract::Recent advances in DNA sequencing technology have made it possible to elucidate the entire genomes of pathogenic bacteria, and advancements in bioinformatic tools have driven comparative studies of these genome sequences. These evaluations are dramatically increasing our ability to make valid considerations of the lim...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:

    authors: McDevitt D,Payne DJ,Holmes DJ,Rosenberg M

    更新日期:2002-01-01 00:00:00

  • Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl-contaminated soil and sediment in the Czech Republic.

    abstract::Two polychlorinated biphenyl-contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. Fro...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00830.x

    authors: Pavlû L,Vosáhlová J,Klierová H,Prouza M,Demnerová K,Brenner V

    更新日期:1999-09-01 00:00:00

  • Characterization of OmpK, GAPDH and their fusion OmpK-GAPDH derived from Vibrio harveyi outer membrane proteins: their immunoprotective ability against vibriosis in large yellow croaker (Pseudosciaena crocea).

    abstract:AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03386.x

    authors: Zhang C,Yu L,Qian R

    更新日期:2007-11-01 00:00:00

  • Evaluating the role of total organic carbon in predicting the treatment efficacy of biosand filters for the removal of Vibrio cholerae in drinking water during startup.

    abstract:AIMS:In biosand filters (BSF), treatment is largely driven by the development of a biolayer (schmutzdecke) which establishes itself during the startup phase. In this study, the effect of changing influent total organic carbon (TOC) loading on the removal efficiency of Vibrio cholerae in laboratory-operated BSFs was qua...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13909

    authors: Danley-Thomson AA,Huang EC,Worley-Morse T,Gunsch CK

    更新日期:2018-09-01 00:00:00

  • Diversity of spore germination in response to inosine and L-alanine and its interaction with NaCl and pH in the Bacillus cereus group.

    abstract:AIMS:Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS:B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 s...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03847.x

    authors: Broussolle V,Gauillard F,Nguyen-The C,Carlin F

    更新日期:2008-10-01 00:00:00

  • Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: mechanisms involved and potential as a biocontrol agent.

    abstract:AIMS:To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. METHODS AND RESULTS:The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual c...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04970.x

    authors: Rodríguez MA,Cabrera G,Gozzo FC,Eberlin MN,Godeas A

    更新日期:2011-05-01 00:00:00

  • Colonization of the developing rhizosphere of sugar beet seedlings by potential biocontrol agents applied as seed treatments.

    abstract:AIMS:Poor colonization of the rhizosphere is a major constraint of seed treatment biological control. The objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01523.x

    authors: Walker R,Rossall S,Asher MJ

    更新日期:2002-01-01 00:00:00

  • Interactions of Salmonella enterica with lettuce leaves.

    abstract:AIMS:To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. METHODS AND RESULTS:Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofil...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04152.x

    authors: Kroupitski Y,Pinto R,Brandl MT,Belausov E,Sela S

    更新日期:2009-06-01 00:00:00

  • Antimicrobial activity of trans-cinnamic acid and commonly used antibiotics against important fish pathogens and nonpathogenic isolates.

    abstract:AIMS:Antibiotics and several other chemicals have been used to prevent fish diseases. However, this situation results in economic loss for the companies in the aquaculture industry and most importantly it pollutes the environment. Cinnamic acid is a naturally occurring aromatic acid and is considered to be safe for hum...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14097

    authors: Yilmaz S,Sova M,Ergün S

    更新日期:2018-09-04 00:00:00

  • Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes.

    abstract::The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01054.x

    authors: Wang Y,McAllister TA,Yanke LJ,Cheeke PR

    更新日期:2000-05-01 00:00:00

  • Antimicrobial activity of SYNBIO® probiotic formulation in pathogens isolated from chronic ulcerative lesions: in vitro studies.

    abstract:AIMS:Probiotics have the ability to enhance the immune system, produce anti-inflammatory action and promote wound healing process. The first aim of the study was to isolate pathogenic micro-organisms from sites of chronic ulcerative lesion. The second aim was to evaluate probiotic efficacy of SYNBIO® (1:1 combination o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14482

    authors: Coman MM,Mazzotti L,Silvi S,Scalise A,Orpianesi C,Cresci A,Verdenelli MC

    更新日期:2020-02-01 00:00:00

  • Bacillus and relatives in foodborne illness.

    abstract::Species of Bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. These organisms have undergone huge taxonomic changes in the last 30 years, with numbers of genera and species now standing at 56 and over 545, respectively. Despite this expansion, relatively ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2011.05204.x

    authors: Logan NA

    更新日期:2012-03-01 00:00:00

  • Shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli in healthy goats in India: occurrence and virulence properties.

    abstract:AIMS:To describe the occurrence and virulence gene pattern of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) in healthy goats of Jammu and Kashmir, India. METHODS AND RESULTS:A total of 220 E. coli strains belonging to 60 different 'O' serogroups was isolated from 206 local (nonmigra...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02759.x

    authors: Wani SA,Samanta I,Munshi ZH,Bhat MA,Nishikawa Y

    更新日期:2006-01-01 00:00:00

  • A novel genotypic test for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates by a multiplex probe array.

    abstract:AIMS:To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. METHODS AND RESULTS:A multiplex probe array was designed for genotypic test to simultaneously screen the mutations ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03350.x

    authors: Zhang SL,Shen JG,Xu PH,Li DX,Sun ZQ,Li L,Yang ZR,Sun Q

    更新日期:2007-10-01 00:00:00

  • Vaginal microbial communities from synchronized heifers and cows with reproductive disorders.

    abstract:AIM:To evaluate changes in the resident microbial population in the cranial vaginal mucosa induced by a progesterone-releasing intravaginal device (PRID) compared to the vaginal microbiota of cows with reproductive disorders. METHODS AND RESULTS:Vaginal discharge was evaluated by clinical examination and a Vaginitis D...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13239

    authors: Gonzalez Moreno C,Fontana C,Cocconcelli PS,Callegari ML,Otero MC

    更新日期:2016-11-01 00:00:00

  • Variation in acid resistance among enterohaemorrhagic Escherichia coli in a simulated gastric environment.

    abstract:AIMS:To compare survival of enterohaemorrhagic Escherichia coli (EHEC) strains of two clonal groups in a simulated gastric environment and to quantify the effect of storage in an acidic food, apple juice, on subsequent survival of EHEC in the simulated gastric environment. METHODS AND RESULTS:To characterize acid resi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03099.x

    authors: Bergholz TM,Whittam TS

    更新日期:2007-02-01 00:00:00

  • Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR.

    abstract:AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04358.x

    authors: Wang L,Li Y,Mustapha A

    更新日期:2009-11-01 00:00:00

  • Influence of avilamycin administration and its subsequent withdrawal on emergence and disappearance of antimicrobial resistance in enterococci in the intestine of broiler chickens.

    abstract:AIMS:To investigate the influence of avilamycin (AVM) administration and its subsequent withdrawal on the emergence and disappearance of AVM-resistant enterococci in the intestine of broiler chickens. METHODS AND RESULTS:Five chicks each of C, L and H groups were given the basal diet, the basal diet supplemented with ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03140.x

    authors: Katsunuma Y,Hanazumi M,Fujisaki H,Minato H,Hashimoto Y,Yonemochi C

    更新日期:2007-04-01 00:00:00

  • The survival of Staphylococcus aureus during the fermentation and storage of yoghurt.

    abstract::The effect of yoghurt culture Rx on the survival of Staphylococcus aureus CCM 5984 added to milk in various concentrations was observed during the fermentation and storage of yoghurt. The end of the fermentation process (3.5 h) was only accompanied by a slight reduction. During the storage of yoghurt at 4 degrees C a ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:

    authors: Pazakova J,Turek P,Laciakova A

    更新日期:1997-05-01 00:00:00

  • Evaluation of ATP bioluminescence-based methods for hygienic assessment in fish industry.

    abstract:AIMS:To evaluate ATP bioluminescence-based hygiene monitoring systems under conditions relevant to fish processing environments. METHODS AND RESULTS:The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14292

    authors: Møretrø T,Normann MA,Saebø HR,Langsrud S

    更新日期:2019-07-01 00:00:00

  • Proposal of a method for the genetic transformation of Gordonia jacobaea.

    abstract:AIMS:Gordonia jacobaea is a recently isolated bacterial species with potential industrial application on account of its ability to store large quantities of trans-canthaxanthin. Its genetic manipulation is, however, difficult and cumbersome owing to the presence of mycolic acids in the cell wall and, especially, becaus...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02806.x

    authors: Veiga-Crespo P,Feijoo-Siota L,de Miguel T,Poza M,Villa TG

    更新日期:2006-03-01 00:00:00

  • Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods.

    abstract:AIMS:This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional culture method. METHODS AND RESULTS:Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water sa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04553.x

    authors: Bonetta S,Bonetta S,Ferretti E,Balocco F,Carraro E

    更新日期:2010-05-01 00:00:00

  • Study of the effects of temperature, pH and yeast extract on growth and exopolysaccharides production by Propionibacterium acidi-propionici on milk microfiltrate using a response surface methodology.

    abstract:AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01310.x

    authors: Gorret N,Maubois JL,Engasser JM,Ghoul M

    更新日期:2001-05-01 00:00:00

  • Survival of Listeria monocytogenes in commercial food-processing equipment cleaning solutions and subsequent sensitivity to sanitizers and heat.

    abstract:AIMS:To determine the ability of Listeria monocytogenes to survive exposure to commercial food-processing equipment cleaning solutions and subsequent treatment with sanitizers or heat. METHODS AND RESULTS:Cells of five strains of L. monocytogenes were suspended in 1% solutions of eight commercial cleaners (pH 7.1-12.5...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01488.x

    authors: Taormina PJ,Beuchat LR

    更新日期:2002-01-01 00:00:00

  • High-yield strain of fusidic acid obtained by atmospheric and room temperature plasma mutagenesis and the transcriptional changes involved in improving its production in fungus Fusidium coccineum.

    abstract:AIMS:To obtain the high-yield strain of fusidic acid, which is produced from fungus Fusidium coccineum and is the only fusidane-type antibiotic that has been used clinically, and confirm the changes in the transcription levels involved in increasing its production. METHODS AND RESULTS:By using the atmospheric and room...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14797

    authors: Huang WW,Ge XY,Huang Y,Chai XT,Zhang L,Zhang YX,Deng LN,Liu CQ,Xu H,Gao J

    更新日期:2020-07-30 00:00:00

  • Effect of lipopeptides of antagonistic strains of Bacillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen Podosphaera fusca.

    abstract:AIMS:To analyse the morphological and ultrastructural effects of lipopeptides of cell-free liquid cultures from the antagonistic Bacillus subtilis strains, UMAF6614 and UMAF6639, on the cucurbit powdery mildew fungus, Podosphaera fusca, conidial germination. METHODS AND RESULTS:Butanolic extracts from cell-free supern...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03323.x

    authors: Romero D,de Vicente A,Olmos JL,Dávila JC,Pérez-García A

    更新日期:2007-10-01 00:00:00

  • Bacillus cereus is common in the environment but emetic toxin producing isolates are rare.

    abstract:AIMS:To determine the incidence of emetic toxin producing Bacillus cereus in soil, animal faeces and selected vegetable produce to compare the results with the previously reported high incidence in rice paddy fields. To examine whether the emetic toxin has antibiotic activity. METHODS AND RESULTS:The incidence of emet...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02764.x

    authors: Altayar M,Sutherland AD

    更新日期:2006-01-01 00:00:00

  • Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt.

    abstract:AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04574.x

    authors: Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

    更新日期:2010-04-01 00:00:00

  • Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217.

    abstract:AIMS:To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. METHODS AND RESULTS:Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A21...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14548

    authors: He H,Hao X,Zhou W,Shi N,Feng J,Han L

    更新日期:2020-04-01 00:00:00