Development of a 5'-nuclease PCR assay for the identification of Escherichia coli strains expressing the flagellar antigen H21 and their detection in food after enrichment.

Abstract:

AIMS:To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C. METHODS AND RESULTS:The fliC allele of STEC O91:H21 strain B2F1 was amplified and sequenced. The nucleotide sequence obtained was compared with fliC genes of E. coli O157:H21, O8:H21 and O113:H21 strains. A pair of oligonucleotide primers and a TaqMan minor groove binder probe specific for fliC-H21 were designed and used in a 5'-nuclease PCR assay. This method was evaluated using a panel of 138 diverse bacterial strains and was shown to be 100% specific for H21. PCR amplification of fliC-H21 from one cell per reaction mixture was possible, and an initial inoculum of 10 STEC H21 colony-forming units per 25 g of ground beef was detected after overnight enrichment. CONCLUSIONS:The PCR assay developed was found to be highly sensitive and specific for the identification and detection of E. coli H21 strains in ground beef. SIGNIFICANCE AND IMPACT OF THE STUDY:The real-time PCR assay targeting the H21 flagellar antigen described here offers a valuable method for the rapid detection and molecular typing of pathogenic STEC H21 strains in food.

journal_name

J Appl Microbiol

authors

Auvray F,Lecureuil C,Taché J,Perelle S,Fach P

doi

10.1111/j.1365-2672.2007.03607.x

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

899-905

issue

3

eissn

1364-5072

issn

1365-2672

pii

JAM3607

journal_volume

104

pub_type

杂志文章
  • Dynamics of living and dead bacterial cells within a mixed-species biofilm during toluene degradation in a biotrickling filter.

    abstract:AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01914.x

    authors: Tresse O,Lescob S,Rho D

    更新日期:2003-01-01 00:00:00

  • Influence of isomalto-oligosaccharides on intestinal microbiota in rats.

    abstract:AIMS:Isomalto-oligosaccharides (IMO) with α(1 --> 6) and α(1 --> 4) glucosidic linkages are produced by enzymatic conversion of starch. IMO are only partially digestible but data on their influence on intestinal microbiota are limited. It was the aim of this study to investigate the effect of IMO diet on intestinal mic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04984.x

    authors: Ketabi A,Dieleman LA,Gänzle MG

    更新日期:2011-05-01 00:00:00

  • Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain.

    abstract:AIMS:To evaluate the prevalence of Cryptosporidium and Giardia in surface water supplies from the province of Alava, northern Spain, and to investigate possible associations among the presence of these pathogenic protozoa with microbiological, physicochemical and atmospheric parameters. METHODS AND RESULTS:A total of ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03193.x

    authors: Carmena D,Aguinagalde X,Zigorraga C,Fernández-Crespo JC,Ocio JA

    更新日期:2007-03-01 00:00:00

  • Apple bagasse as a substrate for the propagation of Patagonian wine yeast biomass.

    abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14216

    authors: Bravo SME,Morales M,Del Mónaco SM,Caballero AC

    更新日期:2019-05-01 00:00:00

  • Recognition of Yersinia enterocolitica multiple strain infection in twin infants using PCR-based DNA fingerprinting.

    abstract:AIMS:Yersinia enterocolitica causes several syndromes in humans. The most common presentation is enterocolitis in children, presenting as fever and diarrhoea. A Y. enterocolitica multiple strain infection in twin infants was investigated. METHODS AND RESULTS:One isolate was recovered from one patient and two morpholog...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01252.x

    authors: Gray JT,WaKabongo M,Campos FE,Diallo AA,Tyndal C,Tucker CA

    更新日期:2001-03-01 00:00:00

  • Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani: an endophyte of Glycyrrhiza glabra.

    abstract:AIMS:Glycyrrhiza glabra is a high-value medicinal plant thriving in biodiversity rich Kashmir Himalaya. The present study was designed to explore the fungal endophytes from G. glabra as a source of bioactive molecules. METHODS AND RESULTS:The extracts prepared from the isolated endophytes were evaluated for anti-micro...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13410

    authors: Shah A,Rather MA,Hassan QP,Aga MA,Mushtaq S,Shah AM,Hussain A,Baba SA,Ahmad Z

    更新日期:2017-05-01 00:00:00

  • Analysis of biocide transport limitation in an artificial biofilm system.

    abstract::An alginate gel bead artificial biofilm system was used to assay biofilm susceptibility to four biocides and to analyse the extent to which each agent penetrated the biofilm. Chlorine, glutaraldehyde, an isothiazolone, and a quaternary ammonium compound were tested on alginate-entrapped Enterobacter aerogenes in gel b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.853529.x

    authors: Stewart PS,Grab L,Diemer JA

    更新日期:1998-09-01 00:00:00

  • Antifungal activity of extracts and purified saponins from the rhizomes of Chamaecostus cuspidatus against Candida and Trichophyton species.

    abstract:AIM:To evaluate the antifungal activity of extracts of Chamaecostus cuspidatus against Candida and Trichophyton species. METHODS AND RESULTS:Crude ethanol extracts of leaves, stems and rhizomes were prepared and evaluated for antimicrobial activity. Only the rhizomes extract (RE) showed antifungal activity but had no ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14783

    authors: Barros Cota B,Batista Carneiro de Oliveira D,Carla Borges T,Cristina Catto A,Valverde Serafim C,Rogelis Aquiles Rodrigues A,Kohlhoff M,Leomar Zani C,Assunção Andrade A

    更新日期:2021-01-01 00:00:00

  • Non-sterile corn steep liquor a novel, cost effective and powerful culture media for Sporosarcina pasteurii cultivation for sand improvement.

    abstract:AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14866

    authors: Babakhani S,Fahmi A,Katebi H,Ouria A,Majnouni-Toutakhane A,Ganbarov K,Kafil HS

    更新日期:2020-10-07 00:00:00

  • Development of a novel selective agar for the isolation and detection of Bacillus anthracis.

    abstract:AIMS:The aim of this study was to develop a novel selective agar for the specific isolation and detection of Bacillus anthracis. METHODS AND RESULTS:Based on published data on antibiotic resistance and susceptibility of B. anthracis and other closely related species of the Bacillus cereus sensu lato group, a new selec...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14615

    authors: Rohde A,Papp S,Feige P,Grunow R,Kaspari O

    更新日期:2020-08-01 00:00:00

  • Characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses.

    abstract:AIMS:To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. METHODS AND RESULTS:One ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01874.x

    authors: Corsetti A,De Angelis M,Dellaglio F,Paparella A,Fox PF,Settanni L,Gobbetti M

    更新日期:2003-01-01 00:00:00

  • Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle.

    abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01727.x

    authors: Lan GQ,Abdullah N,Jalaludin S,Ho YW

    更新日期:2002-01-01 00:00:00

  • Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration.

    abstract:AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12796

    authors: Bravo-Ferrada BM,Gonçalves S,Semorile L,Santos NC,Tymczyszyn EE,Hollmann A

    更新日期:2015-06-01 00:00:00

  • Relevance of microbial coculture fermentations in biotechnology.

    abstract::The purpose of this article is to review coculture fermentations in industrial biotechnology. Examples for the advantageous utilization of cocultures instead of single cultivations include the production of bulk chemicals, enzymes, food additives, antimicrobial substances and microbial fuel cells. Coculture fermentati...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2009.04659.x

    authors: Bader J,Mast-Gerlach E,Popović MK,Bajpai R,Stahl U

    更新日期:2010-08-01 00:00:00

  • High-throughput screening of a collection of known pharmacologically active small compounds for inhibitors of Salmonella invasion and intracellular replication.

    abstract:AIMS:The aim of this study was to screen a chemical library consisting of over 1200 pharmacologically active, already approved off-patent compounds, to determine whether any of the compounds reduced or eliminated the invasion or intracellular replication phenotypes of Salmonella enterica serovar Typhimurium (S. Typhimu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13890

    authors: Ridge Y,Landini P,Thompson A

    更新日期:2018-09-01 00:00:00

  • Influence of three microbial feed additives of Megasphaera elsdenii, Saccharomyces cerevisiae and Lactobacillus spp. on ruminal methane and carbon dioxide production, and biofermentation kinetics.

    abstract:AIMS:This study was performed to investigate the effects of Megasphaera elsdenii (Me), Saccharomyces cerevisiae (SC) and lactic acid bacteria (FP - Lactobacillus fermentum plus Lactobacillus plantarum) alone or in combination on biogas production and ruminal biofermentation parameter in a heterofermenter system. METHO...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14990

    authors: Direkvandi E,Mohammadabadi T,Salem AZM

    更新日期:2021-01-07 00:00:00

  • Antifungal modes of action of tea tree oil and its two characteristic components against Botrytis cinerea.

    abstract:AIMS:The essential oil of Melaleuca alternifolia (tea tree) has been evaluated as a potential eco-friendly antifungal agent against Botrytis cinerea. In this study, we investigated the antifungal activity and mode of action of tea tree oil (TTO) and its components against B. cinerea. METHODS AND RESULTS:Of the compone...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12939

    authors: Yu D,Wang J,Shao X,Xu F,Wang H

    更新日期:2015-11-01 00:00:00

  • The response of Leuconostoc mesenteroides to low external oxidoreduction potential generated by hydrogen gas.

    abstract:AIMS:The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated. METHODS AND RESULTS:Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions (Eh7: +200 mV and -400 mV) using nitrogen and hydrogen as reducing agents. Growth wa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01832.x

    authors: Bourel G,Henini S,Diviès C,Garmyn D

    更新日期:2003-01-01 00:00:00

  • Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.

    abstract::Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00339.x

    authors: Margolles A,Mayo B,de los Reyes-Gavilán CG

    更新日期:1998-02-01 00:00:00

  • Genetic characterization of non-spoilage variant isolated from beer-spoilage Lactobacillus brevis ABBC45.

    abstract:AIMS:To characterize the non-spoilage variant obtained from beer-spoilage Lactobacillus brevis ABBC45C and to identify a potential genetic marker capable of discriminating beer-spoilage L. brevis strains from non-spoilers. METHODS AND RESULTS:A non-spoilage variant was obtained from beer-spoilage L. brevis ABBC45C by ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02244.x

    authors: Suzuki K,Koyanagi M,Yamashita H

    更新日期:2004-01-01 00:00:00

  • Immunogenicity of recombinant outer membrane porin protein and protective efficacy against lethal challenge with Bordetella bronchiseptica in rabbits.

    abstract:AIMS:The outer membrane porin protein (OMPP) of Bordetella bronchiseptica is an important adhesion factor and protective immunogen. The aim of this study was to verify the immunogenicity of recombinant OMPP and its protective efficacy against a lethal challenge with B. bronchiseptica in rabbits. METHODS AND RESULTS:So...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14451

    authors: Zhang H,Zhang H,Xiong B,Fan G,Cao Z

    更新日期:2019-12-01 00:00:00

  • Research of quality indices for cold-smoked salmon using a stepwise multiple regression of microbiological counts and physico-chemical parameters.

    abstract:AIMS:The aim of the study was to assess the relationships between the remaining shelf-life (RSL) of cold-smoked salmon and various microbiological and physico-chemical parameters, using a multivariate data analysis in the form of stepwise forward multiple regression. METHODS AND RESULTS:Thirteen batches of French cold...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01283.x

    authors: Leroi F,Joffraud JJ,Chevalier F,Cardinal M

    更新日期:2001-04-01 00:00:00

  • Transmission of Salmonella enterica serotype Typhimurium in poultry with and without antimicrobial selective pressure.

    abstract:AIMS:To determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of Salmonella in poultry. METHODS AND RESULTS:Eight pens housed 12 broiler chicks each. Two chicks in four of the pens were inoculated with a Salm. Typhimurium strain resistant to 12 an...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03036.x

    authors: Bauer-Garland J,Frye JG,Gray JT,Berrang ME,Harrison MA,Fedorka-Cray PJ

    更新日期:2006-12-01 00:00:00

  • Microbial aerosol generation during laboratory accidents and subsequent risk assessment.

    abstract:AIM:To quantify microbial aerosols generated by a series of laboratory accidents and to use these data in risk assessment. METHODS AND RESULTS:A series of laboratory accident scenarios have been devised and the microbial aerosol generated by them has been measured using a range of microbial air samplers. The accident ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02798.x

    authors: Bennett A,Parks S

    更新日期:2006-04-01 00:00:00

  • Immunodetection of the toxic portion of Vip3A reveals differential temporal regulation of its secretion among Bacillus thuringiensis strains.

    abstract:AIMS:To devise a protocol for heterologous expression and purification of a partial toxic portion of the Bacillus thuringiensis (Bt) vegetative insecticidal protein Vip3A and using it as an antigen for anti-Vip3A polyclonal antibody development. Also, to evaluate the regulation of Vip3A secretion into culture supernata...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13775

    authors: Argôlo-Filho RC,Loguercio LL

    更新日期:2018-08-01 00:00:00

  • Comparison of selective and non-selective enrichment media in the detection of Listeria monocytogenes from meat containing Listeria innocua.

    abstract:AIMS:This study investigated whether the higher incidence of recovery from meat of Listeria innocua compared with L. monocytogenes could be due to the laboratory media used, leading to an artificially lower detection of the pathogenic species, L. monocytogenes. METHODS AND RESULTS:Minced beef was inoculated with L. mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01336.x

    authors: Duffy G,Walsh D,Sheridan JJ,Logue CM,Harrington D,Blair IS,McDowell DA

    更新日期:2001-06-01 00:00:00

  • Composition of semen and foreskin mucosa aerobic microbiota and its impact on sperm parameters of captive collared peccaries (Pecari tajacu).

    abstract:AIM:To evaluate the bacterial composition of collared peccary semen and foreskin mucosa, and to verify the sensitivity of isolates to antimicrobials used in semen conservation and to Aloe vera gel, which is an alternative external cryoprotectant. METHODS AND RESULTS:Nine foreskin mucosa and ejaculate samples from adul...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14663

    authors: Santos CS,Silva AM,Maia KM,Rodrigues GSO,Feijó FMC,Alves ND,Oliveira MF,Silva AR

    更新日期:2020-09-01 00:00:00

  • Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model.

    abstract:AIMS:To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. METHODS AND RESULTS:Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05155.x

    authors: Zhen YH,Fang R,Ding C,Jin LJ,Li XY,Diao YP,Shu XH,Ma XC,Xu YP

    更新日期:2011-12-01 00:00:00

  • Diversity of antifungal and plant-associated Serratia plymuthica strains.

    abstract::A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01064.x

    authors: Berg G

    更新日期:2000-06-01 00:00:00

  • Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide.

    abstract:AIMS:To assess the efficacy of a β-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. METHODS AND RESULTS:Three different doses (0.5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/jam.12713

    authors: Andrés-Barranco S,Vico JP,Grilló MJ,Mainar-Jaime RC

    更新日期:2015-02-01 00:00:00