Abstract:
AIMS:The outer membrane porin protein (OMPP) of Bordetella bronchiseptica is an important adhesion factor and protective immunogen. The aim of this study was to verify the immunogenicity of recombinant OMPP and its protective efficacy against a lethal challenge with B. bronchiseptica in rabbits. METHODS AND RESULTS:Soluble rOMPP was successfully expressed in Escherichia coli, and the purified recombinant protein was mixed with the ISA 201 VG adjuvant to prepare a subunit vaccine for B. bronchiseptica. Rabbits were immunized with the rOMPP subunit vaccine and then infected with the virulent B. bronchiseptica strain QDBb01. Rabbits immunized with the subunit vaccine were completely protected compared to the control group, and the protective effect was obviously better than that of the inactivated whole-cell vaccine. Moreover, analysis of the immunization duration showed that the rOMPP subunit vaccine provided immune protection for at least 4 months after the second immunization. CONCLUSIONS:The rOMPP subunit vaccine completely protected rabbits from a subsequent B. bronchiseptica challenge. SIGNIFICANCE AND IMPACT OF THE STUDY:The results will provide key information for the development of a safe and effective recombinant subunit vaccine against B. bronchiseptica in rabbits.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Zhang H,Zhang H,Xiong B,Fan G,Cao Zdoi
10.1111/jam.14451subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1646-1655issue
6eissn
1364-5072issn
1365-2672journal_volume
127pub_type
杂志文章abstract:AIMS:To develop species-specific monitoring techniques for rapid detection of Bacteroides and Parabacteroides inhabiting the mouse intestine by fluorescence in situ hybridization. METHODS AND RESULTS:The specificity of oligonucleotide probes was evaluated by fluorescence whole-cell hybridization. Oligonucleotide probe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05039.x
更新日期:2011-07-01 00:00:00
abstract::Heterotrophic plate count (HPC) bacteria are naturally present in all aqueous environments. These bacteria undergo multiplication cycles in drinking water, especially in closed containers (bottled water) or in tap water when chlorine levels are dissipated, such as in dead ends in water mains or household plumbing. A s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00134.x
更新日期:1997-04-01 00:00:00
abstract:AIMS:We have been for a species of thermophilic bacteria that can effectively decompose collagen and collagen peptides that tend to be hard-to-degrade proteins because of their high content of proline residues. This study focused upon the enzymatic degradation of prolyl peptides by thermophilic bacteria. METHODS AND R...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02210.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03813.x
更新日期:2008-09-01 00:00:00
abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12456
更新日期:2014-06-01 00:00:00
abstract::Traditional microbiological methods are still used extensively for analysis of micro-organisms in water. However, they are inefficient due to a high labour input requirement, a low sample capacity, and often a long time lag before results are available. Analytical stages involving incubation and growth (enrichments an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.1998.tb05300.x
更新日期:1998-12-01 00:00:00
abstract:AIMS:To determine the effects of supplemented copper (Cu2+) on growth and viability of strains used as starters and adjunct cultures for Emmental cheese manufacture. METHODS AND RESULTS:Thirteen strains belonging to Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus rhamnosus, Streptococcus thermophilu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03849.x
更新日期:2008-10-01 00:00:00
abstract:AIMS:(i) To determine the aflatoxin B1 (AFB1 ) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB1 at each gastrointestinal tract (GIT-saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14101
更新日期:2019-01-01 00:00:00
abstract:AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03018.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatograp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03488.x
更新日期:2007-12-01 00:00:00
abstract::This review is an account of experiences of two research teams (from Italy to Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. This review covers th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13548
更新日期:2017-12-01 00:00:00
abstract:AIM:The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RE...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03756.x
更新日期:2008-08-01 00:00:00
abstract:AIMS:The rise of antibiotic resistance pushes the pharmaceutical industry to continually search for substances with new structures and novel mechanisms of action. Many environmental niches are still to be explored as sources of antimicrobials. In this paper, we assess the antimicrobial potential of gut microbes of spri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13899
更新日期:2018-09-01 00:00:00
abstract:AIMS: To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. M...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05321.x
更新日期:2012-08-01 00:00:00
abstract:AIMS:To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. METHODS AND RESULTS:The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and sub...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13254
更新日期:2016-11-01 00:00:00
abstract:AIMS:To evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile Aeromonas strains. METHODS AND RESULTS:Aeromonas strains were identified to genomospecies level by the 16S rDNA restriction fragment length polymorphism (RFLP) method and characterized phenotypically...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01784.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04770.x
更新日期:2010-10-01 00:00:00
abstract:AIMS:The aim of this work was to investigate the effects of biodynamic management with and without the addition of green manure, in comparison with organic management, on the microbiota in vineyards soil. METHODS AND RESULTS:High throughput sequencing was used to compare the taxonomic structure of the soil bacterial a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13606
更新日期:2017-12-01 00:00:00
abstract:AIMS:We investigated in vitro activity of polyene drugs (amphotericin B, Amp B; and nystatin, NYS;) in combination with ethylene diamine tetra acetic acid (EDTA) against 8 pathogenic isolates of Aspergillus spp. METHODS AND RESULTS:The intensity of the drug interactions was assessed using Fractional Inhibitory Concent...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12576
更新日期:2014-09-01 00:00:00
abstract:AIM:Nano-biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. METHODS AND RESULTS:The pigment extracted from D. maris ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14889
更新日期:2020-10-08 00:00:00
abstract:AIMS:Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium). METHODS AND RESULTS:A deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
abstract:AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14790
更新日期:2020-07-20 00:00:00
abstract:AIMS:Root rot caused by a group of fungi is a serious disease in mulberry. This study aims to identify and characterize Rhizopus oryzae and other fungal species associated with root rot of mulberry in India. METHODS AND RESULTS:Rotted root samples were collected from the mulberry gardens from four states of Southern I...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2020-12-04 00:00:00
abstract:AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04056.x
更新日期:2009-03-01 00:00:00
abstract::Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01177.x
更新日期:2000-11-01 00:00:00
abstract:AIM:Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of CLEs and their combinations agains...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14070
更新日期:2019-01-01 00:00:00
abstract:AIMS:To characterize airborne virus-like particles isolated from two cheese production plants in order to reveal their complexity in terms of viral communities and microbial genes potentially mobilized by viruses. METHODS AND RESULTS:Airborne virus-like particles have been isolated from Grana Padano and Gorgonzola PDO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
abstract:AIMS:To study the effect of the type of antibiotic used in medicated diets against pathogens and the feeding level on the microbial biodiversity in the rabbit caecum. METHODS AND RESULTS:Three groups of eight does were given a diet unsupplemented (NAB) or with 100 ppm of bacitracin (BAC) or tiamulin (TIA). Litter size...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03309.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:Tilletia controversa is an internationally quarantined pathogenic fungus that causes dwarf bunt of wheat and is similar to Tilletia caries in both teliospore morphology and genetic structure. This study developed a rapid and sensitive immunofluorescence method for differentiating the teliospores of T. controversa ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12703
更新日期:2015-02-01 00:00:00
abstract:AIM:The aim of this study was to evaluate the physicochemical properties of the crude pectinase activity from three Bacillus isolates of ruminant dung origin and study their synergism with crude xylanases from the same Bacillus spp. and a commercial cellulase to evaluate their accessory role in improved biomass sacchar...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13718
更新日期:2018-05-01 00:00:00