Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.).

Abstract:

AIMS:To evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile Aeromonas strains. METHODS AND RESULTS:Aeromonas strains were identified to genomospecies level by the 16S rDNA restriction fragment length polymorphism (RFLP) method and characterized phenotypically by the API 20E and API Zym systems and by conventional tube or plate methods. 16S rDNA RFLP analysis showed that the strains belonged to five species, Aeromonas bestiarum (5), Aerom. salmonicida (13), Aerom. veronii (11), Aerom. sobria (6) and Aerom. encheleia (2). Most strains of Aerom. bestiarum (80%) and Aerom. salmonicida (85%) could be separated by growth at 4 and 42 degrees C, autoagglutination after boiling, reaction for lipase (C14) and naphthol-AS-BI-phosphohydrolase. All strains of Aerom. veronii corresponded to Aerom. veronii biotype sobria and could be separated from Aerom. sobria by citrate utilization, growth at 37 and 42 degrees C, amygdalin and cellobiose fermentation. All strains of Aerom. bestiarum and most strains of Aerom. salmonicida (76.9%) and Aerom. veronii (63.6%) were pathogenic for carp. CONCLUSIONS:The biochemical identification of carp Aeromonas strains is not entirely clear. Some association between Aeromonas species, phenotypic profile and specific disease signs was observed. SIGNIFICANCE AND IMPACT OF THE STUDY:The results will be useful for ichthyopathology laboratories in the diagnosis of motile aeromonad septicaemia in carp.

journal_name

J Appl Microbiol

authors

Kozińska A,Figueras MJ,Chacon MR,Soler L

doi

10.1046/j.1365-2672.2002.01784.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

1034-41

issue

6

eissn

1364-5072

issn

1365-2672

pii

1784

journal_volume

93

pub_type

杂志文章
  • Bacterial succession during curing process of a skate (Dipturus batis) and isolation of novel strains.

    abstract:AIMS:To study the succession of cultivated and uncultivated microbes during the traditional curing process of skate. METHODS AND RESULTS:The microbial diversity was evaluated by sequencing 16Sr RNA clone libraries and cultivation in variety of media from skate samples taken periodically during a 9-day curing process. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05349.x

    authors: Reynisson E,Thornór Marteinsson V,Jónsdóttir R,Magnússon SH,Hreggvidsson GO

    更新日期:2012-08-01 00:00:00

  • Accelerated ripening of Kedong sufu with autochthonous starter cultures Kocuria rosea KDF3 and its protease KP3 as adjuncts.

    abstract:AIMS:Application of autochthonous strain Kocuria rosea KDF3 and its protease KP3 as adjuncts for acceleration of Kedong sufu ripening. METHODS AND RESULTS:Kedong sufu was manufactured using autochthonous cultures (batch A), K. rosea KDF3 plus autochthonous cultures (batch B) and protease KP3 plus autochthonous culture...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12433

    authors: Feng Z,Chen H,Lv XT,Deng HL,Chen X,Li JJ,Guo L

    更新日期:2014-04-01 00:00:00

  • Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration.

    abstract:AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12796

    authors: Bravo-Ferrada BM,Gonçalves S,Semorile L,Santos NC,Tymczyszyn EE,Hollmann A

    更新日期:2015-06-01 00:00:00

  • A Streptococcus uberis transposon mutant screen reveals a negative role for LiaR homologue in biofilm formation.

    abstract:AIMS:The environmental pathogen Streptococcus uberis causes intramammary infections in dairy cows. Because biofilm growth might contribute to Strep. uberis mastitis, we conducted a biological screen to identify genes potentially involved in the regulation of biofilm growth. METHODS AND RESULTS:By screening a transposo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12664

    authors: Salomäki T,Karonen T,Siljamäki P,Savijoki K,Nyman TA,Varmanen P,Iivanainen A

    更新日期:2015-01-01 00:00:00

  • Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR.

    abstract:AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatm...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04354.x

    authors: Búfalo MC,Figueiredo AS,de Sousa JP,Candeias JM,Bastos JK,Sforcin JM

    更新日期:2009-11-01 00:00:00

  • Surface hydrophobicity, viability and efficacy in biological control of Penicillium oxalicum spores produced in aerial and submerged culture.

    abstract::The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01189.x

    authors: Pascual S,De Cal A,Magan N,Melgarejo P

    更新日期:2000-11-01 00:00:00

  • Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model.

    abstract:AIMS:To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. METHODS AND RESULTS:Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05155.x

    authors: Zhen YH,Fang R,Ding C,Jin LJ,Li XY,Diao YP,Shu XH,Ma XC,Xu YP

    更新日期:2011-12-01 00:00:00

  • Analysis of environmental Escherichia coli isolates for virulence genes using the TaqMan PCR system.

    abstract:AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.02023.x

    authors: Davis KC,Nakatsu CH,Turco R,Weagant SD,Bhunia AK

    更新日期:2003-01-01 00:00:00

  • Effects of temperature on metal tolerance and the accumulation of Zn and Pb by metal-tolerant fungi isolated from urban runoff treatment wetlands.

    abstract:AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04082.x

    authors: Purchase D,Scholes LN,Revitt DM,Shutes RB

    更新日期:2009-04-01 00:00:00

  • Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.

    abstract:AIMS:Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA)...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05189.x

    authors: Chee JY,Lau NS,Samian MR,Tsuge T,Sudesh K

    更新日期:2012-01-01 00:00:00

  • Membrane disruption and anti-quorum sensing effects of synergistic interaction between Lavandula angustifolia (lavender oil) in combination with antibiotic against plasmid-conferred multi-drug-resistant Escherichia coli.

    abstract:AIM:The aim of this study was to investigate the mode of action of the lavender essential oil (LV) on antimicrobial activity against multi-drug-resistant Escherichia coli J53 R1 when used singly and in combination with piperacillin. METHOD AND RESULTS:In the time-kill analysis, a complete killing of bacteria was obser...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12444

    authors: Yap PS,Krishnan T,Yiap BC,Hu CP,Chan KG,Lim SH

    更新日期:2014-05-01 00:00:00

  • A phytobeneficial strain Planomicrobium sp. MSSA-10 triggered oxidative stress responsive mechanisms and regulated the growth of pea plants under induced saline environment.

    abstract:AIMS:The study was planned to characterize Planomicrobium sp. MSSA-10 for plant-beneficial traits and to evaluate its inoculation impact on physiology of pea plants under different salinity levels. METHODS AND RESULTS:Strain MSSA-10 was isolated from pea rhizosphere and identified by the analysis of 16S rRNA gene sequ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13732

    authors: Shahid M,Akram MS,Khan MA,Zubair M,Shah SM,Ismail M,Shabir G,Basheer S,Aslam K,Tariq M

    更新日期:2018-06-01 00:00:00

  • Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability.

    abstract:AIMS:To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. METHODS AND RESULTS:After adaptive evolution in acetic acid, a clone (Y8A) was selected for its to...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13917

    authors: Gurdo N,Novelli Poisson GF,Juárez ÁB,Rios de Molina MC,Galvagno MA

    更新日期:2018-09-01 00:00:00

  • Rotavirus vaccine stability in the aquatic environment.

    abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13021

    authors: Moresco V,Damazo NA,Barardi CR

    更新日期:2016-02-01 00:00:00

  • The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract.

    abstract:AIMS:The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. METHODS AND RESULTS:Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydroly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04180.x

    authors: Aires A,Mota VR,Saavedra MJ,Rosa EA,Bennett RN

    更新日期:2009-06-01 00:00:00

  • Drought degree constrains the beneficial effects of a fungal endophyte on Atractylodes lancea.

    abstract:AIMS:Plants, fungal endophytes (FEs) and the changing environment interact with each other forming an interlaced network. This study evaluates nonadditive and interactive effects of the FE Acremonium strictum and drought treatment on Atractylodes lancea plantlets. METHODS AND RESULTS:By applying FEs (meristem cultures...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12615

    authors: Yang T,Ma S,Dai CC

    更新日期:2014-11-01 00:00:00

  • A unique lantibiotic, thermophilin 1277, containing a disulfide bridge and two thioether bridges.

    abstract:AIMS:To identify the chemical structure of a bacteriocin, thermophilin 1277, produced by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277 was purified and partial N-terminal sequence analysis revealed 6 unidentified amino acids amongst 31 amino acids residues. A 2.7-kbp region containing the t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04059.x

    authors: Kabuki T,Uenishi H,Seto Y,Yoshioka T,Nakajima H

    更新日期:2009-03-01 00:00:00

  • Phages of Lactobacillus casei/paracasei: response to environmental factors and interaction with collection and commercial strains.

    abstract:AIM:To investigate the influence of several environmental factors on the viability and cell-adsorption for two Lactobacillus casei/paracasei bacteriophages (PL-1 and J-1). METHODS AND RESULTS:Both phages showed a remarkably high specificity of species, sharing similar host spectra. Two phages and four sensitive strain...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02767.x

    authors: Capra ML,Quiberoni A,Reinheimer J

    更新日期:2006-02-01 00:00:00

  • Modelling salinity inhibition effects during biodegradation of perchlorate.

    abstract:AIMS:To determine the mathematical kinetic rates and mechanisms of acclimated perchlorate (ClO)-reducing microbial cultures by incorporating a term to relate the inhibitory effect of high salinity during biological reduction of concentrated perchlorate solutions. METHODS AND RESULTS:Salt toxicity associated with the b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02950.x

    authors: Park C,Marchand EA

    更新日期:2006-07-01 00:00:00

  • Biodiversity of the microbiota in Spodoptera exigua (Lepidoptera: Noctuidae).

    abstract:AIMS:Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14190

    authors: Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang S,Cui J

    更新日期:2019-04-01 00:00:00

  • Detection of the nifH gene of Methanobrevibacter smithii: a potential tool to identify sewage pollution in recreational waters.

    abstract:AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02989.x

    authors: Ufnar JA,Wang SY,Christiansen JM,Yampara-Iquise H,Carson CA,Ellender RD

    更新日期:2006-07-01 00:00:00

  • Temperature-assisted high hydrostatic pressure inactivation of Staphylococcus aureus in a ham model system: evaluation in selective and nonselective medium.

    abstract:AIMS:The purpose of this study was to investigate the inactivation kinetics of Staphylococcus aureus in a ham model system by high hydrostatic pressure at ambient (25 degrees C) and selected temperatures (45, 55 degrees C). Selective [Baird Parker (BP) agar] and nonselective [brain heart infusion (BHI) agar] growth med...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03698.x

    authors: Tassou CC,Panagou EZ,Samaras FJ,Galiatsatou P,Mallidis CG

    更新日期:2008-06-01 00:00:00

  • Prevalence, phenotypic traits and molecular characterization of emetic toxin-producing Bacillus cereus strains isolated from human stools in Korea.

    abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05277.x

    authors: Chon JW,Kim JH,Lee SJ,Hyeon JY,Song KY,Park C,Seo KH

    更新日期:2012-05-01 00:00:00

  • Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii.

    abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03042.x

    authors: Cenci G,Trotta F,Caldini G

    更新日期:2006-12-01 00:00:00

  • Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models.

    abstract:AIMS:The pathogenic potential of Arcobacter butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays. METHODS AND RESULTS:Five of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12979

    authors: Karadas G,Bücker R,Sharbati S,Schulzke JD,Alter T,Gölz G

    更新日期:2016-01-01 00:00:00

  • Preliminary analysis of Chloroflexi populations in full-scale UASB methanogenic reactors.

    abstract:AIMS:The phylum Chloroflexi is frequently found in high abundance in methanogenic reactors, but their role is still unclear as most of them remain uncultured and understudied. Hence, a detailed analysis was performed in samples from five up-flow anaerobic sludge blanket (UASB) full-scale reactors fed different industri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14115

    authors: Bovio P,Cabezas A,Etchebehere C

    更新日期:2019-02-01 00:00:00

  • Diversity, dynamics and activity of bacterial populations in 'Registered Designation of Origin' Salers cheese by single-strand conformation polymorphism analysis of 16S rRNA genes.

    abstract:AIMS:The aim of this work was to measure the dynamic global metabolic activities of the microbial community during ripening of RDO Salers cheese by using a direct molecular approach. METHODS AND RESULTS:A culture-independent approach including PCR, reverse transcriptase PCR (RT-PCR) and single strand conformation poly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02575.x

    authors: Duthoit F,Tessier L,Montel MC

    更新日期:2005-01-01 00:00:00

  • A 210-min solid phase cytometry test for the enumeration of Escherichia coli in drinking water.

    abstract::A 210-min-test for the enumeration of Escherichia coli in drinking water is described, based on solid phase cytometry (SPC) and a two-step enzymatic procedure for fluorescence labelling of single cells and small microcolonies. The test involves membrane filtration through a 25-mm black polyester filter, induction of b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01125.x

    authors: Van Poucke SO,Nelis HJ

    更新日期:2000-09-01 00:00:00

  • Effect of acclimation medium on cell viability, membrane integrity and ability to consume malic acid in synthetic wine by oenological Lactobacillus plantarum strains.

    abstract:AIMS:The aim of this work was to evaluate the effect of acclimation on the viability, membrane integrity and the ability to consume malic acid of three oenological strains of Lactobacillus plantarum. METHODS AND RESULTS:Cultures in the stationary phase were inoculated in an acclimation medium (Accl.) containing 0, 6 o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12372

    authors: Bravo-Ferrada BM,Tymczyszyn EE,Gómez-Zavaglia A,Semorile L

    更新日期:2014-02-01 00:00:00

  • Production of biogenic amines and divercin V41 in cold smoked salmon inoculated with Carnobacterium divergens V41, and specific detection of this strain by multiplex-PCR.

    abstract:AIMS:The objective of this study was to determine the technological behaviour (implantation and biogenic amines production) of Carnobacterium divergens V41, an anti-Listeria bacteriocin producer (divercin V41), after inoculation in cold smoked salmon (CSS). METHODS AND RESULTS:Implantation of the strain was followed b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01561.x

    authors: Connil N,Prévost H,Dousset X

    更新日期:2002-01-01 00:00:00