Abstract：
AIMS:To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. METHODS AND RESULTS:Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg(-1) for measuring survival rate and 10 mg kg(-1) for cytokine measurement. The survival rate of mice treated with 200 mg kg(-1) specific IgY or 5 mg kg(-1) dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly (P < 0.05) decreased tumour necrosis factor-α (TNF-α) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-α than dexamethasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia. CONCLUSIONS:The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-α and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver. SIGNIFICANCE AND IMPACT OF THE STUDY:The specific IgY has potential for the treatment of LPS-induced endotoxemia.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Zhen YH,Fang R,Ding C,Jin LJ,Li XY,Diao YP,Shu XH,Ma XC,Xu YPdoi
10.1111/j.1365-2672.2011.05155.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
1524-32issue
6eissn
1364-5072issn
1365-2672journal_volume
111pub_type
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