Decontamination of a hard surface contaminated with Bacillus anthracisΔSterne and B. anthracis Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO2).

Abstract:

AIMS:To evaluate the inactivation of Bacillus anthracisΔSterne and Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO(2)) and compare two sporulation and decontamination methods with regard to cost, safety and technical constraints. METHODS AND RESULTS:Spores were prepared via agar and broth methods and subsequently inoculated and dried onto clean, autoclave-sterilized glass coupons. Bacillus anthracis spore inactivation efficacy was evaluated using the modified three-step method (AOAC 2008.05) and a single-tube extraction method. Spores (7·0 ± 0·5 logs) were inactivated within 1 min at room temperature using freshly prepared eClO(2). Bacillus anthracisΔSterne spores decreased in size after eClO(2) treatment as measured using a Beckman Coulter Multisizer. CONCLUSIONS:eClO(2) saturation of a hard surface was an effective B. anthracis sporicide. Broth sporulation and the single-tube extraction method required less time and fewer steps, yielded a higher percentage of phase-bright spores and showed higher spore recovery efficiency compared with AOAC 2008.05, making it more amenable to biosafety level 3 (BSL3) testing of virulent spores. SIGNIFICANCE AND IMPACT OF THE STUDY:Two test methods demonstrated the sporicidal efficacy of eClO(2). A new single-tube extraction test protocol for decontaminants was introduced.

journal_name

J Appl Microbiol

authors

Buhr TL,Young AA,Minter ZA,Wells CM,Shegogue DA

doi

10.1111/j.1365-2672.2011.05122.x

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

1057-64

issue

5

eissn

1364-5072

issn

1365-2672

journal_volume

111

pub_type

杂志文章
  • Gluconacin from Gluconacetobacter diazotrophicus PAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria.

    abstract:AIMS:This study aimed to explore the possibility of using the Gluconacin from Gluconacetobacter diazotrophicus strain PAL5 in the biological control of diverse sugarcane phytopathogenic bacteria. METHODS AND RESULTS:An in silico analysis was first employed to determine the phylogenetic relationship between Gram-negati...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14074

    authors: Oliveira MM,Ramos ETA,Drechsel MM,Vidal MS,Schwab S,Baldani JI

    更新日期:2018-08-22 00:00:00

  • Heat and chlorine resistance of a soil Acanthamoeba sp. cysts in water.

    abstract:AIMS:The study established the inactivation kinetic parameters of an Acanthamoeba cyst isolate subjected to heating and chlorination. METHODS AND RESULTS:A strain of Acanthamoeba was isolated and purified from an area surrounding a pilot food plant. Mature cysts (14 days) were subjected to heat inactivation studies at...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14600

    authors: Gabriel AA,Panaligan DC

    更新日期:2020-08-01 00:00:00

  • Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi.

    abstract:AIMS:The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. METHODS AND RESULTS:Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological proce...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01294.x

    authors: Gomes RC,Sêmedo LT,Soares RM,Linhares LF,Ulhoa CJ,Alviano CS,Coelho RR

    更新日期:2001-04-01 00:00:00

  • Effects of the kinetics of water potential variation on bacteria viability.

    abstract::The effect of the kinetics of water potential variation (psi) on the viability of bacteria subjected to hyperosmotic stresses in water-glycerol solution was studied. The three bacteria used were Lactobacillus plantarum L-73, Leuconostoc mesenteroides LM057 and Escherichia coli TG1. These strains were submitted to a fi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.1997.tb03303.x

    authors: Poirier I,Maréchal PA,Gervais P

    更新日期:1997-01-01 00:00:00

  • Development of antimicrobial nanoemulsion-based delivery systems against selected pathogenic bacteria using a thymol-rich Thymus daenensis essential oil.

    abstract:AIMS:Thymol-rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water solubility and high vapour pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in wate...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13541

    authors: Ghaderi L,Moghimi R,Aliahmadi A,McClements DJ,Rafati H

    更新日期:2017-10-01 00:00:00

  • Ochratoxin A removal in synthetic and natural grape juices by selected oenological Saccharomyces strains.

    abstract:AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02385.x

    authors: Bejaoui H,Mathieu F,Taillandier P,Lebrihi A

    更新日期:2004-01-01 00:00:00

  • Impact of pulsed light on cellular activity of Salmonella enterica.

    abstract:AIMS:The objective of this study was a comprehensive characterization of physiological changes of Salmonella enterica induced by intense broad spectrum pulsed light (PL). After exposing the bacteria to this nonthermal decontamination technology on a gel surface, multiple viability parameters beyond culturability were a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13231

    authors: Kramer B,Wunderlich J,Muranyi P

    更新日期:2016-10-01 00:00:00

  • Comparative typing of Pseudomonas species isolated from the aquatic environment in Greece by SDS-PAGE and RAPD analysis.

    abstract:AIMS:Three broadly used typing methods were employed in order to assess and compare the identification and classification of environmental Pseudomonas strains. The reproducibility, typeability and discriminatory power of the methods were also compared to evaluate their application. Finally, the potential impact on publ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02691.x

    authors: Sazakli E,Leotsinidis M,Vantarakis A,Papapetropoulou M

    更新日期:2005-01-01 00:00:00

  • Production of massoia lactone by Aureobasidium pullulans YTP6-14 isolated from the Gulf of Thailand and its fragrant biosurfactant properties.

    abstract:AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13598

    authors: Luepongpattana S,Thaniyavarn J,Morikawa M

    更新日期:2017-12-01 00:00:00

  • Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus.

    abstract:AIMS:To evaluate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. METHODS AND RESULTS:Specific IgY was produced by immunizing hens with formaldehyde-killed Staph. aureus, using a bacterial strain known to cause mastitis. The IgY, of 94% purity, was obtained from yo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03920.x

    authors: Zhen YH,Jin LJ,Guo J,Li XY,Li Z,Fang R,Xu YP

    更新日期:2008-11-01 00:00:00

  • Variability and functional role of chromosomal sequences in 16SrI-B subgroup phytoplasmas including aster yellows and related strains.

    abstract:AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01809.x

    authors: Botti S,Bertaccini A

    更新日期:2003-01-01 00:00:00

  • Determination of antimicrobial resistance and virulence gene signatures in surface water isolates of Escherichia coli.

    abstract:AIMS:To determine the occurrence of Escherichia coli harbouring virulence markers of shiga- or entero-toxins and resistance to antimicrobials in surface waters. METHODS AND RESULTS:Surface water samples were collected at six locations of the river Gomti. E. coli isolates (n = 90) were characterized for their pathogeni...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03879.x

    authors: Ram S,Vajpayee P,Tripathi U,Singh RL,Seth PK,Shanker R

    更新日期:2008-12-01 00:00:00

  • Strain-dependent production of selected bioactive compounds by Cyanobacteria belonging to the Arthrospira genus.

    abstract:AIMS:To investigate the synthesis of selected bioactive compounds in various strains belonging to the Arthrospira genus. METHODS AND RESULTS:The synthesis of vitamin C, polyphenols, vitamin B12, fatty acids and selected volatile compounds (acetone and acetaldehyde) was investigated in six strains belonging to the Arth...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12897

    authors: Satora P,Barwińska-Sendra A,Duda-Chodak A,Wajda Ł

    更新日期:2015-09-01 00:00:00

  • A repeatable laboratory method for testing the efficacy of biocides against toilet bowl biofilms.

    abstract:AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01342.x

    authors: Pitts B,Willse A,McFeters GA,Hamilton MA,Zelver N,Stewart PS

    更新日期:2001-07-01 00:00:00

  • Adaptation of Pseudomonas aeruginosa ATCC 15442 to didecyldimethylammonium bromide induces changes in membrane fatty acid composition and in resistance of cells.

    abstract::The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00770.x

    authors: Méchin L,Dubois-Brissonnet F,Heyd B,Leveau JY

    更新日期:1999-05-01 00:00:00

  • Structural organization and dynamics of exopolysaccharide matrix and microcolonies formation by Streptococcus mutans in biofilms.

    abstract:AIMS:To investigate the structural organization and dynamics of exopolysaccharides (EPS) matrix and microcolonies formation by Streptococcus mutans during the biofilm development process. METHODS AND RESULTS:Biofilms of Strep. mutans were formed on saliva-coated hydroxyapatite (sHA) discs in the presence of glucose or...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04616.x

    authors: Xiao J,Koo H

    更新日期:2010-06-01 00:00:00

  • Lactic acid bacteria isolated from canine faeces.

    abstract:AIMS:Lactic acid bacteria (LAB) were isolated and sequenced from the faeces of healthy dogs. Five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. METHODS AND RESULTS:LAB were found in 67% (14/21) of the canine faeces samples when plated on La...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02884.x

    authors: Beasley SS,Manninen TJ,Saris PE

    更新日期:2006-07-01 00:00:00

  • Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii.

    abstract:AIMS:Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii. METHODS AND RESULTS:Germ-free Artemia nauplii were cultured using Baker's yeast and dead Aer. hydrophila. Live Aer. hydrophila ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04862.x

    authors: Gunasekara RA,Cornillie P,Casteleyn C,De Spiegelaere W,Sorgeloos P,Simoens P,Bossier P,Van den Broeck W

    更新日期:2011-01-01 00:00:00

  • A review of anthrax in Canada and implications for research on the disease in northern bison.

    abstract::During the first half of the century, the majority of anthrax outbreaks in Canada occurred in the southern portions of Ontario and Quebec and were often associated with pastures contaminated by effluent from textile industries dealing with imported animal materials. In 1952, introduction of Federal regulations requiri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2672.1999.00872.x

    authors: Dragon DC,Elkin BT,Nishi JS,Ellsworth TR

    更新日期:1999-08-01 00:00:00

  • Influence of arginine on the growth, arginine metabolism and amino acid consumption profiles of Streptococcus thermophilus T1C2 in controlled pH batch fermentations.

    abstract:AIMS:The aim of this study was to elucidate the effect of arginine on the growth, arginine metabolism and amino acid consumption profiles of Streptococcus thermophilus T1C2. METHODS AND RESULTS:The growth kinetics, intracellular pH, extracellular osmotic pressure, expression of key genes in the arginine metabolism pat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13221

    authors: Huang S,Ai ZW,Sun XM,Liu GF,Zhai S,Zhang M,Chen H,Feng Z

    更新日期:2016-09-01 00:00:00

  • Efficacy of liquid spray decontaminants for inactivation of Bacillus anthracis spores on building and outdoor materials.

    abstract:AIMS:To obtain data on the efficacy of various liquid and foam decontamination technologies to inactivate Bacillus anthracis Ames and Bacillus subtilis spores on building and outdoor materials. METHODS AND RESULTS:Spores were inoculated onto test coupons and positive control coupons of nine different materials. Six di...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04980.x

    authors: Wood JP,Choi YW,Rogers JV,Kelly TJ,Riggs KB,Willenberg ZJ

    更新日期:2011-05-01 00:00:00

  • Differences in major bacterial populations in the intestines of mature broilers after feeding virginiamycin or bacitracin methylene disalicylate.

    abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12960

    authors: Neumann AP,Suen G

    更新日期:2015-12-01 00:00:00

  • A novel genotypic test for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates by a multiplex probe array.

    abstract:AIMS:To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. METHODS AND RESULTS:A multiplex probe array was designed for genotypic test to simultaneously screen the mutations ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03350.x

    authors: Zhang SL,Shen JG,Xu PH,Li DX,Sun ZQ,Li L,Yang ZR,Sun Q

    更新日期:2007-10-01 00:00:00

  • 16S ribosomal DNA analysis of the faecal lactobacilli composition of human subjects consuming a probiotic strain Lactobacillus acidophilus NCFM.

    abstract:AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01767.x

    authors: Sui J,Leighton S,Busta F,Brady L

    更新日期:2002-01-01 00:00:00

  • Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain.

    abstract:AIMS:To evaluate the prevalence of Cryptosporidium and Giardia in surface water supplies from the province of Alava, northern Spain, and to investigate possible associations among the presence of these pathogenic protozoa with microbiological, physicochemical and atmospheric parameters. METHODS AND RESULTS:A total of ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03193.x

    authors: Carmena D,Aguinagalde X,Zigorraga C,Fernández-Crespo JC,Ocio JA

    更新日期:2007-03-01 00:00:00

  • Adaptive changes in geranylgeranyl pyrophosphate synthase gene expression level under ethanol stress conditions in Oenococcus oeni.

    abstract:AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12351

    authors: Cafaro C,Bonomo MG,Salzano G

    更新日期:2014-01-01 00:00:00

  • Lactate is mainly fermented to butyrate by human intestinal microfloras but inter-individual variation is evident.

    abstract:AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02605.x

    authors: Bourriaud C,Robins RJ,Martin L,Kozlowski F,Tenailleau E,Cherbut C,Michel C

    更新日期:2005-01-01 00:00:00

  • Lactobacillus helveticus heterogeneity in natural cheese starters: the diversity in phenotypic characteristics.

    abstract::The study of wild strains from natural habitats is a useful means of understanding better the heterogeneity within a species of biotechnological importance, and of obtaining atypical isolates with unknown capabilities. In the present research carried out on different Lactobacillus helveticus strains isolated from natu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00312.x

    authors: Fortina MG,Nicastro G,Carminati D,Neviani E,Manachini PL

    更新日期:1998-01-01 00:00:00

  • Effects of graded levels of tannin-containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production.

    abstract:AIMS:This study was carried out to determine the effect of graded levels of tannin-containing tropical tree leaves, Autocarpus integrifolis, Azardirachta indica and Ficus bengalensis, on the in vitro rumen fermentation pattern, total protozoa and methane suppression in order to establish the optimum dose of these leave...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12723

    authors: Bhatta R,Saravanan M,Baruah L,Prasad CS

    更新日期:2015-03-01 00:00:00

  • High-throughput screening of a collection of known pharmacologically active small compounds for inhibitors of Salmonella invasion and intracellular replication.

    abstract:AIMS:The aim of this study was to screen a chemical library consisting of over 1200 pharmacologically active, already approved off-patent compounds, to determine whether any of the compounds reduced or eliminated the invasion or intracellular replication phenotypes of Salmonella enterica serovar Typhimurium (S. Typhimu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13890

    authors: Ridge Y,Landini P,Thompson A

    更新日期:2018-09-01 00:00:00