Abstract:
AIMS:The study established the inactivation kinetic parameters of an Acanthamoeba cyst isolate subjected to heating and chlorination. METHODS AND RESULTS:A strain of Acanthamoeba was isolated and purified from an area surrounding a pilot food plant. Mature cysts (14 days) were subjected to heat inactivation studies at 71, 76, 81, 86 and 91°C; and chlorination at 100, 200, 300, 400 and 500 ppm. The decimal reduction times (D-values) at 71, 76, 81, 86 and 91°C were 18·31, 9·26, 7·35, 4·52 and 1·81 min respectively. The calculated thermal resistance constant (z-value) was 21·32°C (R2 = 0·96-0·97). The D-value in 100, 200, 300, 400 and 500 ppm chlorine-treated water were 47·17, 25·06, 24·51, 23·70 and 18·55 min respectively. The chlorine resistance constant (z-value) was 1179 ppm chlorine (R2 = 0·65-0·74). CONCLUSIONS:Results demonstrated high resistance of the isolated Acanthamoeba cysts towards the common methods applied in ensuring food and food processing environment sanitation. SIGNIFICANCE AND IMPACT OF THE STUDY:The resistance parameters of the test organisms established in this study may be used in the establishment of Sanitation Standard Operating Procedures (SSOPs), which are often based on inactivation of bacteria. These SSOPs could render better protection to food and food processing environments.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Gabriel AA,Panaligan DCdoi
10.1111/jam.14600subject
Has Abstractpub_date
2020-08-01 00:00:00pages
453-464issue
2eissn
1364-5072issn
1365-2672journal_volume
129pub_type
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journal_title:Journal of applied microbiology
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