Effects of xylulokinase activity on ethanol production from D-xylulose by recombinant Saccharomyces cerevisiae.

Abstract:

AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of the glyceraldehyde 3-phosphate dehydrogenase promoter and terminator. Specific xylulose consumption rate was enhanced by the increased specific XK activity, resulting from the introduction of the XKS1 into S. cerevisiae. In batch and fed-batch cultivations, the recombinant strains resulted in twofold higher ethanol concentration and 5.3- to six-fold improvement in the ethanol production rate compared with the host strain S. cerevisiae. CONCLUSIONS:An effective conversion of xylulose to xylulose 5-phosphate catalysed by XK in S. cerevisiae was considered to be essential for the development of an efficient and accelerated ethanol fermentation process from xylulose. SIGNIFICANCE AND IMPACT OF THE STUDY:Overexpression of the XKS1 gene made xylulose fermentation process accelerated to produce ethanol through the pentose phosphate pathway.

journal_name

J Appl Microbiol

authors

Lee TH,Kim MD,Park YC,Bae SM,Ryu YW,Seo JH

doi

10.1046/j.1365-2672.2003.02055.x

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

847-52

issue

4

eissn

1364-5072

issn

1365-2672

pii

2055

journal_volume

95

pub_type

杂志文章
  • Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894.

    abstract:AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05263.x

    authors: Wang L,Hu X,Tao G,Wang X

    更新日期:2012-05-01 00:00:00

  • Filter-based PNA in situ hybridization for rapid detection, identification and enumeration of specific micro-organisms.

    abstract:AIMS:A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. METHODS AND RESULTS:The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of time. The microcolonie...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01230.x

    authors: Perry-O'Keefe H,Stender H,Broomer A,Oliveira K,Coull J,Hyldig-Nielsen JJ

    更新日期:2001-02-01 00:00:00

  • Characterization of OmpK, GAPDH and their fusion OmpK-GAPDH derived from Vibrio harveyi outer membrane proteins: their immunoprotective ability against vibriosis in large yellow croaker (Pseudosciaena crocea).

    abstract:AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03386.x

    authors: Zhang C,Yu L,Qian R

    更新日期:2007-11-01 00:00:00

  • Mechanisms of killing of spores of Bacillus subtilis by dimethyldioxirane.

    abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore resistance to and killing by a novel sporicide, dimethyldioxirane (DMDO) that was generated in situ from acetone and potassium peroxymonosulfate at neutral pH. METHODS AND RESULTS:Spores of B. subtilis were effectively killed by DMDO. Rates of killing by DMDO...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03000.x

    authors: Paul M,Atluri S,Setlow B,Setlow P

    更新日期:2006-11-01 00:00:00

  • In vitro activity of antimicrobial-impregnated catheters against biofilms formed by KPC-producing Klebsiella pneumoniae.

    abstract:AIM:To evaluate the activity and effectiveness of impregnated central venous catheters (CVC) against Klebsiella pneumoniae biofilms. METHODS AND RESULTS:The antimicrobial activity and durability of impregnated-CVCs were evaluated over time and the size of zones of inhibition (ZI) was measured. Biofilm formation was ob...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14372

    authors: de Sousa JKT,Haddad JPA,de Oliveira AC,Vieira CD,Dos Santos SG

    更新日期:2019-10-01 00:00:00

  • Efficacy of liquid spray decontaminants for inactivation of Bacillus anthracis spores on building and outdoor materials.

    abstract:AIMS:To obtain data on the efficacy of various liquid and foam decontamination technologies to inactivate Bacillus anthracis Ames and Bacillus subtilis spores on building and outdoor materials. METHODS AND RESULTS:Spores were inoculated onto test coupons and positive control coupons of nine different materials. Six di...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04980.x

    authors: Wood JP,Choi YW,Rogers JV,Kelly TJ,Riggs KB,Willenberg ZJ

    更新日期:2011-05-01 00:00:00

  • Beneficial effects of probiotics in upper respiratory tract infections and their mechanical actions to antagonize pathogens.

    abstract::Probiotics are live micro-organisms with beneficial effects on human health, which have the ability to counteract infections at different locations of the body. Clinical trials have shown that probiotics can be used as preventive and therapeutic agents in upper respiratory tract infections (URTIs) and otitis. Their me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2012.05394.x

    authors: Popova M,Molimard P,Courau S,Crociani J,Dufour C,Le Vacon F,Carton T

    更新日期:2012-12-01 00:00:00

  • The phenotypic characterization of yeast strains to stresses inherent to wine fermentation in warm climates.

    abstract:AIMS:Climate change is exerting an increasingly profound effect on grape composition, microbiology, chemistry and the sensory aspects of wine. Identification of autochthonous yeasts tolerant to stress could help to alleviate this effect. METHODS AND RESULTS:Tolerance to osmotic pressure, ethanol and pH of 94 Saccharom...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13139

    authors: García M,Greetham D,Wimalasena TT,Phister TG,Cabellos JM,Arroyo T

    更新日期:2016-07-01 00:00:00

  • Biodiversity analysis by polyphasic study of marine bacteria associated with biocorrosion phenomena.

    abstract:AIMS:A polyphasic approach was used to study the biodiversity bacteria associated with biocorrosion processes, in particular sulfate-reducing bacteria (SRB) and thiosulfate-reducing bacteria (TRB) which are described to be particularly aggressive towards metallic materials, notably via hydrogen sulfide release. METHOD...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04643.x

    authors: Boudaud N,Coton M,Coton E,Pineau S,Travert J,Amiel C

    更新日期:2010-07-01 00:00:00

  • Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, Lact. fermentum, Lact. rhamnosus, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weissella minor and related taxa isolated from kocho

    abstract:AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01202.x

    authors: Nigatu A

    更新日期:2000-12-01 00:00:00

  • PCR detection and 16S rRNA sequence-based phylogeny of a novel Propionibacterium acidipropionici applicable for enhanced fermentation of high moisture corn.

    abstract:AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02282.x

    authors: Romanov MN,Bato RV,Yokoyama MT,Rust SR

    更新日期:2004-01-01 00:00:00

  • Evaluation of the efficiency of peracetic acid in the disinfection of sewage effluents.

    abstract:AIMS:Evaluation of the efficiency of peracetic acid in the disinfection of wastewater in a large treatment plant. METHODS AND RESULTS:Over a period of 18 months 30 sample collections were made, each consisting of three samples taken from: raw incoming sewage, secondary effluent (after 10-12 h) and secondary effluent d...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01451.x

    authors: Stampi S,De Luca G,Zanetti F

    更新日期:2001-11-01 00:00:00

  • Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis.

    abstract::Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01177.x

    authors: Ishii K,Fukui M,Takii S

    更新日期:2000-11-01 00:00:00

  • Pseudomonas aeruginosa biofilm disruption using microbial surfactants.

    abstract:AIMS:To establish the ability of the rhamnolipids biosurfactants from Pseudomonas aeruginosa, in the presence and absence of caprylic acid and ascorbic acid, to disrupt bacterial biofilms, compared with the anionic alkyl sulphate surfactant Sodium dodecyl sulphate (SDS). METHODS AND RESULTS:Pseudomonas aeruginosa ATCC...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13049

    authors: Díaz De Rienzo MA,Stevenson PS,Marchant R,Banat IM

    更新日期:2016-04-01 00:00:00

  • Biodiversity of the microbiota in Spodoptera exigua (Lepidoptera: Noctuidae).

    abstract:AIMS:Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14190

    authors: Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang S,Cui J

    更新日期:2019-04-01 00:00:00

  • Influence of avilamycin administration and its subsequent withdrawal on emergence and disappearance of antimicrobial resistance in enterococci in the intestine of broiler chickens.

    abstract:AIMS:To investigate the influence of avilamycin (AVM) administration and its subsequent withdrawal on the emergence and disappearance of AVM-resistant enterococci in the intestine of broiler chickens. METHODS AND RESULTS:Five chicks each of C, L and H groups were given the basal diet, the basal diet supplemented with ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03140.x

    authors: Katsunuma Y,Hanazumi M,Fujisaki H,Minato H,Hashimoto Y,Yonemochi C

    更新日期:2007-04-01 00:00:00

  • Photobacterium damselae subsp. piscicida responds to antimicrobial peptides through phage-shock-protein A (PspA)-related extracytoplasmic stress response system.

    abstract:AIMS:To investigate whether Photobacterium damselae subsp. piscicida (Phdp) can sense and directly respond to the presence of cationic antimicrobial peptides (AMPs). METHODS AND RESULTS:We performed proteomic methodologies to investigate the responsive proteins of Phdp on exposure to AMP Q6. Proteins significantly alt...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12672

    authors: Tsai WC,Kuo TY,Lin CY,Lin JC,Chen WJ

    更新日期:2015-01-01 00:00:00

  • Gluconacin from Gluconacetobacter diazotrophicus PAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria.

    abstract:AIMS:This study aimed to explore the possibility of using the Gluconacin from Gluconacetobacter diazotrophicus strain PAL5 in the biological control of diverse sugarcane phytopathogenic bacteria. METHODS AND RESULTS:An in silico analysis was first employed to determine the phylogenetic relationship between Gram-negati...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14074

    authors: Oliveira MM,Ramos ETA,Drechsel MM,Vidal MS,Schwab S,Baldani JI

    更新日期:2018-08-22 00:00:00

  • Monitoring of Staphylococcus xylosus DSM 20266 added as starter during fermentation and ripening of soppressata molisana, a typical Italian sausage.

    abstract:AIMS:"Soppressata molisana", a fermented sausage produced in southern Italy, is commonly obtained without starter addition. However, the use of starter cultures is more and more recommended in meat fermentation processes in order to guarantee stable production performance. In this study, the survival of the Staphylococ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01512.x

    authors: Di Maria S,Basso AL,Santoro E,Grazia L,Coppola R

    更新日期:2002-01-01 00:00:00

  • Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus.

    abstract:AIMS:The medicinal fungi Inonotus xeranticus and Phellinus linteus in the family Hymenochaetaceae have been used as traditional medicines for the treatment of various diseases. However, the compound responsible for the antioxidant activity is still unknown. Therefore, this study was conducted to characterize the antiox...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03737.x

    authors: Jung JY,Lee IK,Seok SJ,Lee HJ,Kim YH,Yun BS

    更新日期:2008-06-01 00:00:00

  • Microbiological characterization and probiotic potential of koko and koko sour water, African spontaneously fermented millet porridge and drink.

    abstract:AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2004.02162.x

    authors: Lei V,Jakobsen M

    更新日期:2004-01-01 00:00:00

  • Research of quality indices for cold-smoked salmon using a stepwise multiple regression of microbiological counts and physico-chemical parameters.

    abstract:AIMS:The aim of the study was to assess the relationships between the remaining shelf-life (RSL) of cold-smoked salmon and various microbiological and physico-chemical parameters, using a multivariate data analysis in the form of stepwise forward multiple regression. METHODS AND RESULTS:Thirteen batches of French cold...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01283.x

    authors: Leroi F,Joffraud JJ,Chevalier F,Cardinal M

    更新日期:2001-04-01 00:00:00

  • Ecophysiological characterization of common food-borne fungi in relation to pH and water activity under various atmospheric compositions.

    abstract::The combined effects of pH, water activity (aw), oxygen (O2) and carbon dioxide (CO2) levels on growth and sporulation of 10 common food-borne fungi were studied. The use of a multivariate statistical method (PLS) for the analysis of data showed that the fungi could be grouped according to their physiological response...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00368.x

    authors: Haasum I,Nielsen PV

    更新日期:1998-03-01 00:00:00

  • The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract.

    abstract:AIMS:The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. METHODS AND RESULTS:Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydroly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04180.x

    authors: Aires A,Mota VR,Saavedra MJ,Rosa EA,Bennett RN

    更新日期:2009-06-01 00:00:00

  • Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase.

    abstract:AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03838.x

    authors: Horakova K,Mlejnkova H,Mlejnek P

    更新日期:2008-10-01 00:00:00

  • Use of selected enterococci and Rhizopus oryzae proteases to hydrolyse wheat proteins responsible for celiac disease.

    abstract:AIMS:This work aimed at using a pool of selected enterococci and fungal proteases to hydrolyse wheat gluten during long-time fermentation. METHODS AND RESULTS:A liquid dough made with wheat flour (20% w/w) was fermented with three Enterococcus strains (dough A) or with the combination of enterococci and Rhizopus oryza...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04008.x

    authors: M'hir S,Rizzello CG,Di Cagno R,Cassone A,Hamdi M

    更新日期:2009-02-01 00:00:00

  • Genotypic characteristics of multidrug-resistant Pseudomonas aeruginosa from hospital wastewater treatment plant in Rio de Janeiro, Brazil.

    abstract:AIMS:To investigate Pseudomonas aeruginosa isolates from a hospital wastewater treatment plant (HWTP), focusing on enzyme-based mechanisms of β-lactams resistance and the genetic relatedness among isolates. METHODS AND RESULTS:Forty-one Ps. aeruginosa strains recovered from a HWTP were identified by amplification of 1...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12792

    authors: Miranda CC,de Filippis I,Pinto LH,Coelho-Souza T,Bianco K,Cacci LC,Picão RC,Clementino MM

    更新日期:2015-06-01 00:00:00

  • Purification and characterization of a branched-chain amino acid aminotransferase from Lactobacillus paracasei subsp. paracasei CHCC 2115.

    abstract:AIM:Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. METHODS AND RESULTS:The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interac...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02163.x

    authors: Thage BV,Rattray FP,Laustsen MW,Ardö Y,Barkholt V,Houlberg U

    更新日期:2004-01-01 00:00:00

  • Use of enzymatic methods for rapid enumeration of coliforms in freshwaters.

    abstract::Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00977.x

    authors: George I,Petit M,Servais P

    更新日期:2000-03-01 00:00:00

  • Bacillus and relatives in foodborne illness.

    abstract::Species of Bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. These organisms have undergone huge taxonomic changes in the last 30 years, with numbers of genera and species now standing at 56 and over 545, respectively. Despite this expansion, relatively ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2011.05204.x

    authors: Logan NA

    更新日期:2012-03-01 00:00:00