Abstract:
AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of the glyceraldehyde 3-phosphate dehydrogenase promoter and terminator. Specific xylulose consumption rate was enhanced by the increased specific XK activity, resulting from the introduction of the XKS1 into S. cerevisiae. In batch and fed-batch cultivations, the recombinant strains resulted in twofold higher ethanol concentration and 5.3- to six-fold improvement in the ethanol production rate compared with the host strain S. cerevisiae. CONCLUSIONS:An effective conversion of xylulose to xylulose 5-phosphate catalysed by XK in S. cerevisiae was considered to be essential for the development of an efficient and accelerated ethanol fermentation process from xylulose. SIGNIFICANCE AND IMPACT OF THE STUDY:Overexpression of the XKS1 gene made xylulose fermentation process accelerated to produce ethanol through the pentose phosphate pathway.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Lee TH,Kim MD,Park YC,Bae SM,Ryu YW,Seo JHdoi
10.1046/j.1365-2672.2003.02055.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
847-52issue
4eissn
1364-5072issn
1365-2672pii
2055journal_volume
95pub_type
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2001.01230.x
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journal_title:Journal of applied microbiology
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