Abstract:
AIMS:To investigate Pseudomonas aeruginosa isolates from a hospital wastewater treatment plant (HWTP), focusing on enzyme-based mechanisms of β-lactams resistance and the genetic relatedness among isolates. METHODS AND RESULTS:Forty-one Ps. aeruginosa strains recovered from a HWTP were identified by amplification of 16S rRNA gene. β-lactamase production was screened by disc diffusion, CHROMagar extended-spectrum β-lactamase (ESBL) and β-lactamase strips. β-lactamase and ESBL producing isolates were investigated by PCR for the presence of ESBL, metallo-β-lactamase and Klebsiella pneumoniae carbapenemase encoding genes. Thirty-four isolates (83%) were resistant to at least one antibiotic belonging to three or more classes. Out of these 34 isolates, 28 (82%) were classified as multidrug-resistant (MDR) and 6 (18%) extensively drug-resistant (XDR). Genetic relatedness by Enterobacterial Repetitive Intergenic Consensus sequence-PCR and Multilocus sequence typing analysis showed 20 distinct profiles and 15 sequencing types respectively. Clonal Complex 244 (CC244) shows the pathogenic potential of this clone carrying MDR and XDR strains from clinical, environmental and hospital waste sources. CONCLUSIONS:Our results suggest that treatment facilities for hospital wastewater can stimulate the increase of antimicrobial resistance bacteria and genes. SIGNIFICANCE AND IMPACT OF THE STUDY:The great genetic diversity of Ps. aeruginosa recovered from HWTP constantly released into aquatic systems allow the spread of antimicrobial-resistant organisms and genes.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Miranda CC,de Filippis I,Pinto LH,Coelho-Souza T,Bianco K,Cacci LC,Picão RC,Clementino MMdoi
10.1111/jam.12792subject
Has Abstractpub_date
2015-06-01 00:00:00pages
1276-86issue
6eissn
1364-5072issn
1365-2672journal_volume
118pub_type
杂志文章abstract:AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04514.x
更新日期:2010-04-01 00:00:00
abstract:AIM:Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. METHODS AND RESULTS:Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14347
更新日期:2019-09-01 00:00:00
abstract:AIMS:To establish the ability of the rhamnolipids biosurfactants from Pseudomonas aeruginosa, in the presence and absence of caprylic acid and ascorbic acid, to disrupt bacterial biofilms, compared with the anionic alkyl sulphate surfactant Sodium dodecyl sulphate (SDS). METHODS AND RESULTS:Pseudomonas aeruginosa ATCC...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13049
更新日期:2016-04-01 00:00:00
abstract:AIMS:Isolate and characterize antifungal peptides exhibiting activity against Byssochlamys fulva H25, a spoilage mould associated with juices and beverages. METHODS AND RESULTS:A bacterium (H215) isolated from honey showed high antifungal activity against B. fulva H25. The antifungal producer strain was identified as ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03797.x
更新日期:2008-09-01 00:00:00
abstract:AIMS:Due to their antimicrobial activity, silver nanoparticles (Ag-NPs) are being increasingly used in a number of industrial products. The accumulation of Ag-NPs in the soil might affect plant growth-promoting rhizobacteria and, in turn, the plants. We describe the effects of Ag-NPs on the soil bacteria Azotobacter vi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12779
更新日期:2015-05-01 00:00:00
abstract:AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03617.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:To investigate the structural organization and dynamics of exopolysaccharides (EPS) matrix and microcolonies formation by Streptococcus mutans during the biofilm development process. METHODS AND RESULTS:Biofilms of Strep. mutans were formed on saliva-coated hydroxyapatite (sHA) discs in the presence of glucose or...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04616.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:The study was planned to characterize Planomicrobium sp. MSSA-10 for plant-beneficial traits and to evaluate its inoculation impact on physiology of pea plants under different salinity levels. METHODS AND RESULTS:Strain MSSA-10 was isolated from pea rhizosphere and identified by the analysis of 16S rRNA gene sequ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13732
更新日期:2018-06-01 00:00:00
abstract:AIMS:To generate stable acid-resistant Bifidobacterium strains isolated from human subjects and characterize the phenotypic changes of the acid-resistant derivatives. METHODS AND RESULTS:The ability of 20 Bifidobacterium strains isolated from human faeces to survive to simulated gastrointestinal transit was evaluated,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03342.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04591.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03018.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04326.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:Production of isomaltulose by newly isolated Klebsiella sp. LX3. METHODS AND RESULTS:The bacterial isolate LX3, which transforms sucrose to isomaltulose and trehalulose, has been isolated from a soil sample in Singapore. Morphological and biochemical analysis, as well as 16s rRNA sequence demonstrated that the is...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02006.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To characterize airborne virus-like particles isolated from two cheese production plants in order to reveal their complexity in terms of viral communities and microbial genes potentially mobilized by viruses. METHODS AND RESULTS:Airborne virus-like particles have been isolated from Grana Padano and Gorgonzola PDO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14046
更新日期:2018-11-01 00:00:00
abstract::Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00339.x
更新日期:1998-02-01 00:00:00
abstract:AIMS:Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. METHODS AND RESULTS:Thirty-five Lactob...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01547.x
更新日期:2002-01-01 00:00:00
abstract::The effect of the kinetics of water potential variation (psi) on the viability of bacteria subjected to hyperosmotic stresses in water-glycerol solution was studied. The three bacteria used were Lactobacillus plantarum L-73, Leuconostoc mesenteroides LM057 and Escherichia coli TG1. These strains were submitted to a fi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1997.tb03303.x
更新日期:1997-01-01 00:00:00
abstract:AIMS:To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. METHODS AND RESULTS:Specific IgY was produced by immunizing hens ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12857
更新日期:2015-08-01 00:00:00
abstract:AIMS:To compare clinical and environmental Aspergillus fumigatus strains on their toxicogenic and immunosuppressive capacity. METHODS AND RESULTS:A total of 51 strains of A. fumigatus isolated from clinical and corn silage samples were assayed. All A. fumigatus strains were assayed for gliotoxin production, therefore ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12673
更新日期:2015-01-01 00:00:00
abstract:AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was de...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02661.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12585
更新日期:2014-10-01 00:00:00
abstract:AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12767
更新日期:2015-05-01 00:00:00
abstract:AIMS:Bacillus cereus sensu lato is a complex group of closely related bacteria, which are commonly present in the natural environment and food products. These organisms may cause food poisoning and spoilage as well as opportunistic infections. Thus far, their resistance to selected antibiotics has been explored only in...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14792
更新日期:2020-07-21 00:00:00
abstract:AIMS:To investigate the effects of the endophyte Bacillus subtilisALB629 on the growth of cacao seedlings at early developmental stage and to evaluate its antimicrobial properties. METHODS AND RESULTS:Germinating cacao seeds were inoculated with ALB629, and seedlings growth was evaluated 30 days later. Significant inc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12485
更新日期:2014-06-01 00:00:00
abstract:AIMS:Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium). METHODS AND RESULTS:A deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14044
更新日期:2018-11-01 00:00:00
abstract::The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00821.x
更新日期:2000-01-01 00:00:00
abstract:AIMS:To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. METHODS AND RESULTS:Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05155.x
更新日期:2011-12-01 00:00:00
abstract:AIMS:This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. METHODS AND RESULTS:Administration of th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13687
更新日期:2018-03-01 00:00:00
abstract:AIMS:To compare survival of enterohaemorrhagic Escherichia coli (EHEC) strains of two clonal groups in a simulated gastric environment and to quantify the effect of storage in an acidic food, apple juice, on subsequent survival of EHEC in the simulated gastric environment. METHODS AND RESULTS:To characterize acid resi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03099.x
更新日期:2007-02-01 00:00:00
abstract:AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04440.x
更新日期:2009-11-01 00:00:00