Abstract:
:The effect of the kinetics of water potential variation (psi) on the viability of bacteria subjected to hyperosmotic stresses in water-glycerol solution was studied. The three bacteria used were Lactobacillus plantarum L-73, Leuconostoc mesenteroides LM057 and Escherichia coli TG1. These strains were submitted to a final water potential of -107.2 MPa, -170.9 MPa and/or -244.7 MPa. In any case the kinetics of water potential variation was found to have a great effect on the cell viability. The application of slow water potential decreases could maintain an important cell viability (about 80-100%) with regard to the corresponding viability observed after a sudden step change for the same final water potential (15-57%). For each strain tested, an optimal dehydration kinetics was determined which depended on the final water potential. The existence of this optimum could be explained thanks to the opposition of two actions affecting cell viability: a positive action relative to the slowness of the water potential variation and a negative action relative to the residence time of cells in a critical range of water potential.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Poirier I,Maréchal PA,Gervais Pdoi
10.1111/j.1365-2672.1997.tb03303.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
101-6issue
1eissn
1364-5072issn
1365-2672journal_volume
82pub_type
杂志文章abstract:AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04056.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:The objective of the study was to compare ultraviolet (u.v.) inactivation kinetics of indigenous aerobic spores in surface water with their laboratory-cultured spore isolates and to investigate the relationship between physicochemical characteristics and u.v. inactivation kinetics of spore isolates. METHODS AND R...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02455.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:Poor colonization of the rhizosphere is a major constraint of seed treatment biological control. The objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01523.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. METHODS AND RESULTS:Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13564
更新日期:2017-11-01 00:00:00
abstract:AIMS:To evaluate the use of relatively low levels of hydrogen peroxide vapour (HPV) for the inactivation of Bacillus anthracis spores within an indoor environment. METHODS AND RESULTS:Laboratory-scale decontamination tests were conducted using bacterial spores of both B. anthracis Ames and Bacillus atrophaeus inoculat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13284
更新日期:2016-12-01 00:00:00
abstract::Antifungal metabolites produced by Bacillus pumilus in Potato Dextrose Broth (PDB) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. The antifungal metabolites inhibited mycelial growth of many species of Aspergillus, Penicillium and Fusarium. They also inhibited production of aflat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00431.x
更新日期:1998-06-01 00:00:00
abstract:AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04358.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04870.x
更新日期:2011-01-01 00:00:00
abstract:AIMS:To determine the 16S rRNA gene fingerprints of Bacillus thuringiensis strains to reveal phylogenetic relationships among them. METHODS AND RESULTS:Using 16S rRNA gene restriction fragment length polymorphisms generated by HindIII and EcoRI, 86 Bacillus thuringiensis strains were classified. This includes 80 B. th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01227.x
更新日期:2001-01-01 00:00:00
abstract:AIMS:The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV-C device to hydrogen peroxide d...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14308
更新日期:2019-08-01 00:00:00
abstract:AIMS:The aim of this study is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A. METHODS AND RESULTS:An A. faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results sho...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13537
更新日期:2017-09-01 00:00:00
abstract::Cerein 7 is a peptidic antibiotic produced by Bacillus cereus Bc7 (CECT 5148) at the end of exponential growth but before sporulation onset. Cerein 7 has a broad spectrum of antibacterial activity against Gram-positive bacteria, but it is inactive against Gram-negative bacteria. The sequence of its amino-terminal end ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01123.x
更新日期:2000-08-01 00:00:00
abstract:AIMS:Application of autochthonous strain Kocuria rosea KDF3 and its protease KP3 as adjuncts for acceleration of Kedong sufu ripening. METHODS AND RESULTS:Kedong sufu was manufactured using autochthonous cultures (batch A), K. rosea KDF3 plus autochthonous cultures (batch B) and protease KP3 plus autochthonous culture...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12433
更新日期:2014-04-01 00:00:00
abstract:AIMS:To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C. METHODS AND RESULTS:The fliC allele of STEC O91:H21 strain B2F1 was amplified and sequence...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03607.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS:Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04212.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract::The effects of nisin and monolaurin, alone and in combination, were investigated on Bacillus licheniformis spores in milk at 37 degrees C. In the absence of inhibitors, germinated spores developed into growing vegetative cells and started sporulation at the end of the exponential phase. In the presence of nisin (25 IU...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00669.x
更新日期:1999-02-01 00:00:00
abstract:AIMS:This study aimed at evaluating the inhibitory effects of various organic extracts of Ocimum basilicum against some species of Bipolaris and Cochliobolus with GC-MS and HPLC analysis. METHODS AND RESULTS:The ethyl acetate extract consisted of methyl cinnamate as the most abundant component, while butylated hydroxy...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13543
更新日期:2017-10-01 00:00:00
abstract::Considerable investment has been made in recent years in improvements to the microbiological quality of urban wastewater discharges to surface waters, particularly in coastal towns, with the aim of reducing the exposure of bathers and surfers to gastrointestinal pathogens. As this source of pollution has come under gr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.94.s1.10.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The study was undertaken to investigate the occurrence of glutamate dehydrogenase activity in different species of lactobacilli, and to determine, in a series of cheese-making trials, the effects of glutamate dehydrogenase-producing adjunct cultures on sensory attribute development during the maturation of cheddar...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03017.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:The goal was to study the effects of sub-minimum inhibitory concentrations (sub-MICs) of amoxicillin (AMX) on various physiological responses and virulence determinants in a commensal strain of Escherichia coli. MATERIALS AND RESULTS:The commensal strain was passaged under various sub-MICs of AMX and its effect o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14987
更新日期:2021-01-02 00:00:00
abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13021
更新日期:2016-02-01 00:00:00
abstract:AIM:To determine the structure of the chimeric chromosome X of bottom-fermenting yeasts. METHODS AND RESULTS:Eight cosmid clones carrying DNA from chromosome X of bottom-fermenting yeasts were selected by end-sequencing. Four of the cosmid clones had Saccharomyces cerevisiae (SC)-type and Saccharomyces bayanus (SB)-ty...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04289.x
更新日期:2009-10-01 00:00:00
abstract:AIMS:The aims of the study were to quantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and to determine the vulnerability to aflatoxin increases after purchase. METHODS AND RESULTS:Aflatoxin concentrations were determin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13448
更新日期:2017-06-01 00:00:00
abstract::A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00455.x
更新日期:1998-06-01 00:00:00
abstract:AIMS:Climate change is exerting an increasingly profound effect on grape composition, microbiology, chemistry and the sensory aspects of wine. Identification of autochthonous yeasts tolerant to stress could help to alleviate this effect. METHODS AND RESULTS:Tolerance to osmotic pressure, ethanol and pH of 94 Saccharom...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13139
更新日期:2016-07-01 00:00:00
abstract:AIMS:To study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. METHODS AND RESULTS:Seed of rice cultivar PSBRc14 was collected from farmers' fields of irrigated lowland in southern Luzon, Philippines. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04268.x
更新日期:2009-09-01 00:00:00
abstract:AIM:To study the microbiology of intensive, in-vessel biodegradation of a mixture of sewage sludge and vegetable food waste. METHODS AND RESULTS:The biodegradation was performed in a closed reactor with the addition of a starter culture of Bacillus thermoamylovorans SW25 under conditions of controlled aeration, stirri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02182.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12992
更新日期:2016-01-01 00:00:00
abstract::A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01064.x
更新日期:2000-06-01 00:00:00