Chimeric types of chromosome X in bottom-fermenting yeasts.

Abstract:

AIM:To determine the structure of the chimeric chromosome X of bottom-fermenting yeasts. METHODS AND RESULTS:Eight cosmid clones carrying DNA from chromosome X of bottom-fermenting yeasts were selected by end-sequencing. Four of the cosmid clones had Saccharomyces cerevisiae (SC)-type and Saccharomyces bayanus (SB)-type chimeric ends, two had SC-type ends and two had SB-type ends. Sequencing revealed that the bottom-fermenting yeast strains in this study had four types of chromosome X: SC-SC, SC-SB, SB-SC and SB-SB. The translocation site in the chimeric chromosome is conserved among bottom-fermenting yeast strains, and was created by homologous recombination within a region of high sequence identity between the SC-type sequence and the SB-type sequence. CONCLUSIONS:Existing bottom-fermenting yeast strains share a common ancestor in which the chimeric chromosome X was generated. SIGNIFICANCE AND IMPACT OF THE STUDY:The knowledge gained in this study sheds light on the evolution of bottom-fermenting yeasts.

journal_name

J Appl Microbiol

authors

Ogata T,Izumikawa M,Tadami H

doi

10.1111/j.1365-2672.2009.04289.x

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

1098-107

issue

4

eissn

1364-5072

issn

1365-2672

pii

JAM4289

journal_volume

107

pub_type

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