Abstract:
AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatography (TLC). Further analysis of the AHL signals using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) indicated the presence of C4-HSL and 3OC8-HSL. The expression of aprX in P. fluorescens 395 was investigated, using a transcriptional fusion between the aprX promoter and a mutated gfp variant gene (gfp[mut3]). The results demonstrated that the activity of the aprX promoter increased dramatically in the late exponential phase of growth, indicating growth phase-dependent regulation. The activity was repressed in an AHL-deficient environment, in which the signal molecules were hydrolysed by the enzyme AHL lactonase. CONCLUSIONS:N-acyl homoserine lactones produced by P. fluorescens 395 were identified to be C4-HSL and 3OC8-HSL. The protease gene in P. fluorescens is regulated by the AHL-based quorum sensing system at a transcriptional level during the late exponential growth phase. SIGNIFICANCE AND IMPACT OF THE STUDY:This study contributes to our understanding of the genetic regulation and ecology of P. fluorescens in the economically important system of food spoilage.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Liu M,Wang H,Griffiths MWdoi
10.1111/j.1365-2672.2007.03488.xsubject
Has Abstractpub_date
2007-12-01 00:00:00pages
2174-84issue
6eissn
1364-5072issn
1365-2672pii
JAM3488journal_volume
103pub_type
杂志文章abstract:AIMS:The goal of this research was the development of a PCR-based assay to identify important decay fungi from wood of hardwood tree species in northern temperate regions. METHODS AND RESULTS:Eleven taxon-specific primers were designed for PCR amplification of either nuclear or mitochondrial ribosomal DNA regions of A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03378.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To investigate the structural organization and dynamics of exopolysaccharides (EPS) matrix and microcolonies formation by Streptococcus mutans during the biofilm development process. METHODS AND RESULTS:Biofilms of Strep. mutans were formed on saliva-coated hydroxyapatite (sHA) discs in the presence of glucose or...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04616.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization. METHODS AND RESULTS:A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01263.x
更新日期:2001-03-01 00:00:00
abstract:AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04082.x
更新日期:2009-04-01 00:00:00
abstract:AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04514.x
更新日期:2010-04-01 00:00:00
abstract:AIMS:Microbial community associated with hydrogen production and volatile fatty acids (VFAs) accumulation was characterized in acidogenic hydrogenesis using molasses wastewater as a feedstock. METHODS AND RESULTS:Hydrogen and VFAs production were measured under an organic loading rate (OLR) from 19 to 35 g-COD l-1 da...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13316
更新日期:2016-12-01 00:00:00
abstract:AIMS:Production of isomaltulose by newly isolated Klebsiella sp. LX3. METHODS AND RESULTS:The bacterial isolate LX3, which transforms sucrose to isomaltulose and trehalulose, has been isolated from a soil sample in Singapore. Morphological and biochemical analysis, as well as 16s rRNA sequence demonstrated that the is...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02006.x
更新日期:2003-01-01 00:00:00
abstract:AIM:The aim of this study was to further characterize a bacterial culture (VUN 10,010) capable of benzo[a]pyrene cometabolism. METHODS AND RESULTS:The bacterial culture, previously characterized as a pure culture of Stenotrophomonas maltophilia (VUN 10,010), was found to also contain another bacterial species (Mycobac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02087.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The study was performed to evaluate the safety of whole and RTE vegetables and to investigate the effectiveness of different preventive strategies for the quality assurance of RTE vegetables collected from three Italian production systems. Producer 1, applied a strict system in compliance with GAP- GMP - HACCP, Pr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04727.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:In biosand filters (BSF), treatment is largely driven by the development of a biolayer (schmutzdecke) which establishes itself during the startup phase. In this study, the effect of changing influent total organic carbon (TOC) loading on the removal efficiency of Vibrio cholerae in laboratory-operated BSFs was qua...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13909
更新日期:2018-09-01 00:00:00
abstract:AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02289.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cyto...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02496.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:This study was performed to analyse the molecular characteristics of genes encoding for the major virulence factors in Bacillus anthracis vaccine strain 'Carbosap' compared with the wild B. anthracis strain, to evaluate the basis of attenuation. METHODS AND RESULTS:The molecular characteristics of the B. anthraci...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01660.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To develop a predictive model for Escherichia coli using deep neural networks. METHODS AND RESULTS:Batch experiments are conducted at different temperatures closer to optimum value (36·5°C, 37°C, 37·5°C, 38°C and 38·5°C) to obtain the growth curves of E .coli K-12. Two primary models namely modified Gompertz and ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14901
更新日期:2020-10-16 00:00:00
abstract:AIMS:Isolates of Candida valida, Rhodotorula glutinis and Trichosporon asahii from the rhizosphere of sugar beet in Egypt were examined for their ability to colonize roots, to promote plant growth and to protect sugar beet from Rhizoctonia solani AG-2-2 diseases, under glasshouse conditions. METHODS AND RESULTS:Root c...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02043.x
更新日期:2004-01-01 00:00:00
abstract::The goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13438
更新日期:2017-07-01 00:00:00
abstract:AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04192.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:To generate stable acid-resistant Bifidobacterium strains isolated from human subjects and characterize the phenotypic changes of the acid-resistant derivatives. METHODS AND RESULTS:The ability of 20 Bifidobacterium strains isolated from human faeces to survive to simulated gastrointestinal transit was evaluated,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03342.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02989.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:To identify native Antarctic bacteria capable of oil degradation at low temperatures. METHODS AND RESULTS:Oil contaminated and pristine soils from Signy Island (South Orkney Islands, Antarctica) were examined for bacteria capable of oil degradation at low temperatures. Of the 300 isolates cultured, Pseudomonas st...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02678.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04440.x
更新日期:2009-11-01 00:00:00
abstract::The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00770.x
更新日期:1999-05-01 00:00:00
abstract:AIMS:Chlamydia trachomatis and herpes simplex virus (HSV) are the most prevalent bacterial and viral sexually transmitted infections. Due to the chronic nature of their infections, they are able to interact with titanium-dioxide (TiO2 ) nanoparticles (NPs) applied as food additives or drug delivery vehicles. The aim of...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13560
更新日期:2017-11-01 00:00:00
abstract::The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00710.x
更新日期:1999-04-01 00:00:00
abstract:AIMS:To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, haemagglutinin 1 (HA1 ). METHODS AND RESULTS:The mucosal and systemic immune response...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12325
更新日期:2013-12-01 00:00:00
abstract:AIM:Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. METHODS AND RESULTS:The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02163.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To evaluate the sensitivity and specificity of the BioFire Diagnostics FilmArray(®) system in combination with their Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft) and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. METHODS AND RESULTS:DNA samples f...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12107
更新日期:2013-04-01 00:00:00
abstract:AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14790
更新日期:2020-07-20 00:00:00
abstract:AIMS:Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). METHODS AND RESULTS:Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. B...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12546
更新日期:2014-08-01 00:00:00
abstract:AIMS:Monitoring of microbial changes during and after application of various disinfection treatments in a model domestic water system. METHODS AND RESULTS:A pilot-scale domestic water system consisting of seven galvanized steel re-circulation loops and copper dead legs was constructed. Culture techniques, confocal las...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02391.x
更新日期:2004-01-01 00:00:00