Abstract:
AIMS:To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, haemagglutinin 1 (HA1 ). METHODS AND RESULTS:The mucosal and systemic immune responses that are triggered by LDL17-pH following the oral administration to 10-day-old chickens were evaluated. The results showed that LDL17-pH could significantly increase the specific anti-HA1 IgA antibody level in the mucosa and the anti-HA1 IgG level in sera. Tissues were isolated from trachea and Peyer's patches(PPs)and caecal tonsils of chickens, and gene expression was analysed via real-time quantitative PCR. CONCLUSIONS:The results showed that LDL17-pH could significantly induce the specific anti-HA1 IgA antibody level in the trachea and intestine and the specific anti-HA1 IgG antibody level in the serum (P < 0·05). Additionally, LDL17-pH was in the capacity to induce the expression of cytokines IFN-γ, TLR-2 and AvBD-9 in the PPs and caecal tonsils. Most importantly, the chickens that were immunized with LDL17-pH were protected against lethal challenge of the H5N1 virus to some extent. SIGNIFICANCE AND IMPACT OF THE STUDY:Therefore, LDL17-pH could be a promising oral vaccine candidate against HPAI.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Wang Z,Yu Q,Fu J,Liang J,Yang Qdoi
10.1111/jam.12325subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1269-77issue
6eissn
1364-5072issn
1365-2672journal_volume
115pub_type
杂志文章abstract:AIMS:The technological properties of 22 micrococcus strains from traditional fermented Kedong sufu were evaluated in order to develop autochthonous starter cultures. METHODS AND RESULTS:The proteolytic, autolytic and lipolytic activity, salt tolerance, production and degradation of the biogenic amines of six Micrococc...
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2009-06-01 00:00:00
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更新日期:2015-08-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:1999-06-01 00:00:00
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更新日期:2004-01-01 00:00:00
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doi:10.1111/j.1365-2672.2009.04210.x
更新日期:2009-07-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:
更新日期:2002-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2004.02218.x
更新日期:2004-01-01 00:00:00
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更新日期:1999-10-01 00:00:00