Abstract:
AIMS:To develop probiotics for the control of vibriosis caused by Vibrio anguillarum and Vibrio ordalii in finfish. METHODS AND RESULTS:Kocuria SM1, isolated from the digestive tract of rainbow trout, was administered orally to rainbow trout (Oncorhynchus mykiss) for 2 weeks at a dose equivalent to c. 10(8) cells per g of feed and then challenged intraperitoneally with V. anguillarum and V. ordalii. Use of SM1 led to a reduction in mortalities to 15-20% compared to 74-80% mortalities in the controls. SM1 stimulated both cellular and humoral immune responses in rainbow trout, by elevation of leucocytes (5.5 +/- 0.8 x 10(6) ml(-1) from 3.7 +/- 0.8 x 10(6) ml(-1)), erythrocytes (1.2 +/- 0.1 x 10(8) ml(-1) from 0.8 +/- 0.1 x 10(8) ml(-1)), protein (23 +/- 4.4 mg ml(-1) from 16 +/- 1.3 mg ml(-1)), globulin (15.7 +/- 0.2 mg ml(-1) from 9.9 +/- 0.1 mg ml(-1)) and albumin (7.3 +/- 0.2 mg ml(-1) from 6.1 +/- 0.1 mg ml(-1)) levels, upregulation of respiratory burst (0.05 +/- 0.01 from 0.02 +/- 0.01), complement (56 +/- 7.2 units ml(-1) from 40 +/- 8.0 units ml(-1)), lysozyme (920 +/- 128.8 units ml(-1) from 760 +/- 115.3 units ml(-1)) and bacterial killing activities. CONCLUSIONS:Kocuria SM1 successfully controlled vibriosis in rainbow trout, and the mode of action reflected stimulation of the host innate immune system. SIGNIFICANCE AND IMPACT OF THE STUDY:Probiotics can contribute a significant role in fish disease control strategies, and their use may replace some of the inhibitory chemicals currently used in fish farms.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Sharifuzzaman SM,Austin Bdoi
10.1111/j.1365-2672.2009.04618.xsubject
Has Abstractpub_date
2010-06-01 00:00:00pages
2162-70issue
6eissn
1364-5072issn
1365-2672pii
JAM4618journal_volume
108pub_type
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