Abstract:
AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences amplified with primers AY19p/m, G35p/m and BB88F1/R1 after RFLP analyses showed a high degree of polymorphism among the strains studied. The ribosomal protein (rp) subgroups B and K, and an undescribed subgroup designated N, were identified. Amplicons obtained with primers AY19p/m and BB88F1/R1, revealed a high and a low degree of polymorphism, respectively. CONCLUSIONS:A probable spacer role could be attributed to the AY19p/m sequence and a possible coding function to the BB88F1/R1 sequence. No relationship was found among genetic polymorphisms, identified by statistical analyses, and epidemiological or biological parameters. SIGNIFICANCE AND IMPACT OF THE STUDY:The analyses of five different genomic sequences of the 35 strains belonging to subgroup 16SrI-B allowed a finer distinction among them, confirming that the polymorphism level of 16S rDNA is too low to be adopted as unique parameter for classification.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Botti S,Bertaccini Adoi
10.1046/j.1365-2672.2003.01809.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
103-10issue
1eissn
1364-5072issn
1365-2672pii
1809journal_volume
94pub_type
杂志文章abstract:AIMS:(i) To analyse the increase in calcium ion uptake caused by several cationic dyes on Candida albicans, (ii) to postulate a mechanism, (iii) to define the effects of Zn ions on the phenomenon, and (iv) to propose the use of the dyes or their derivatives against C. albicans. METHODS AND RESULTS:Cells were grown in ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14092
更新日期:2018-08-28 00:00:00
abstract:AIM:The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RE...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03756.x
更新日期:2008-08-01 00:00:00
abstract:AIMS:To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. METHODS AND RESULTS:Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combinati...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02835.x
更新日期:2006-04-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02491.x
更新日期:2005-01-01 00:00:00
abstract::Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04100.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01727.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous Enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. METHODS AND RESULTS:Six Enterococcus faecalis strains were paired with 15 other microbial isolates to form mix...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03187.x
更新日期:2007-05-01 00:00:00
abstract:AIMS:The cell-surface display of chitinase Chi9602ΔSP was constructed on Bacillus thuringiensis BMB171 by using two repeat N-terminal regions of autolysin (Mbgn)2 as the anchoring motif. This study aimed to prepare a chitinolytic whole-cell B. thuringiensis biocatalyst with improved catalytic and antifungal activities....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13333
更新日期:2017-01-01 00:00:00
abstract:AIMS:To evaluate a standard aerosolization method for uniformly depositing threat-representative spores onto surfaces. METHODS AND RESULTS:Lyophilized Bacillus anthracis ΔSterne spores, coated in silica, were aerosolized into a containment chamber and deposited onto nine surface types by two independent laboratories. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12509
更新日期:2014-07-01 00:00:00
abstract:AIMS:Lactic acid bacteria (LAB) were isolated and sequenced from the faeces of healthy dogs. Five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. METHODS AND RESULTS:LAB were found in 67% (14/21) of the canine faeces samples when plated on La...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02884.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:In China, apple production areas are largely from the coastal to inland areas and across varied climate zones. However, the relationship among soil micro-organisms, environmental factors and fruit quality has not been clearly confirmed in orchards. Here we attempted to identify the variation of soil bacteria in th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14878
更新日期:2020-10-04 00:00:00
abstract:AIMS:Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia pastoris. And to obtain a purified and characterized recombinant laccase to analyse the biotechnological application potential. METHODS AND RESULTS:Lac I was cloned an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13720
更新日期:2018-06-01 00:00:00
abstract:AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04056.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04578.x
更新日期:2010-05-01 00:00:00
abstract:AIMS:To identify and characterize adhesion-associated proteins in the potential probiotic Lactobacillus fermentum BCS87. METHODS AND RESULTS:Protein suspensions obtained from the treatment of Lact. fermentum BCS87 with 1 mol 1(-1) LiCl were analysed by Western blotting using HRP-labelled porcine mucus and mucin. Two a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04368.x
更新日期:2009-12-01 00:00:00
abstract:AIMS:To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. METHODS AND RESULTS:After adaptive evolution in acetic acid, a clone (Y8A) was selected for its to...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13917
更新日期:2018-09-01 00:00:00
abstract:AIMS:Environmental contamination plays an important role in the transmission of infections, especially healthcare-associated infections. Disinfection transiently reduces contamination, but surfaces can rapidly become re-contaminated. Antimicrobial surfaces may partially overcome that limitation. The antimicrobial activ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12308
更新日期:2013-11-01 00:00:00
abstract:AIMS:To develop a DNA microarray for easy and fast detection of trichothecene- and moniliformin-producing Fusarium species. METHOD AND RESULTS:A DNA microarray was developed for detection and identification of 14 trichothecene- and moniliformin-producing species of the fungal genus Fusarium. The array could also diffe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03165.x
更新日期:2007-04-01 00:00:00
abstract:AIMS:The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan equine encephalitis virus (VEEV), on two material types. METHODS AND RESULTS:Francisella tularensis (F.t.) and VEEV were inoculated (c. 1 × 108 colony-form...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14532
更新日期:2020-04-01 00:00:00
abstract:AIMS:To assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of Satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03694.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. METHODS AND RESULTS:A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic propertie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01711.x
更新日期:2002-01-01 00:00:00
abstract:AIM:The aim of this study was to investigate the mode of action of the lavender essential oil (LV) on antimicrobial activity against multi-drug-resistant Escherichia coli J53 R1 when used singly and in combination with piperacillin. METHOD AND RESULTS:In the time-kill analysis, a complete killing of bacteria was obser...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12444
更新日期:2014-05-01 00:00:00
abstract:AIMS:To investigate the bactericidal activity of lactoferrin-derived peptides and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the influence on virulence factors of P. aeruginosa. METHODS AND RESULTS:Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides isolated fr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04751.x
更新日期:2010-10-01 00:00:00
abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:Antibiotics and several other chemicals have been used to prevent fish diseases. However, this situation results in economic loss for the companies in the aquaculture industry and most importantly it pollutes the environment. Cinnamic acid is a naturally occurring aromatic acid and is considered to be safe for hum...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14097
更新日期:2018-09-04 00:00:00
abstract:AIMS:To identify aerobic mesophilic bacteria isolated from coreboard, kitchen roll paper and food packaging boards containing recycled fibres and to create a rapid fingerprint-based database for their identification. METHODS AND RESULTS:A total of 197 isolates and 20 relevant type strains were characterized by automat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01803.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To determine the presence of Vibrio cholerae in different areas of Argentina in three sample types, to determine the composition of planktonic communities in areas at which this pathogen was detected and to characterize the virulence properties and antimicrobial resistance of the recovered environmental isolates. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03468.x
更新日期:2007-12-01 00:00:00
abstract:AIMS:To determine whether strains of Geobacillus stearothermophilus isolated from a milk powder manufacturing plant were different in their ability to form biofilms and produce spores. In addition, this study evaluated whether there were other physiological characteristics that could differentiate these strains. METHO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12366
更新日期:2014-02-01 00:00:00
abstract:AIMS:To identify and characterize the main contaminant yeast species detected in fuel-ethanol production plants in Northeast region of Brazil by using molecular methods. METHODS AND RESULTS:Total DNA from yeast colonies isolated from the fermentation must of industrial alcohol plants was submitted to PCR fingerprintin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03082.x
更新日期:2007-02-01 00:00:00
abstract:AIMS:To evaluate the protective efficacy of Pediococcus pentosaceus GS4 in cadmium (Cd)-induced toxicity in Swiss albino mice. METHODS AND RESULTS:The previously reported probiotic P. pentosaceus GS4 interaction with Cd was evaluated experimentally. Swiss albino mice were segregated randomly into three different group...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14195
更新日期:2019-04-01 00:00:00