Abstract:
AIMS:To investigate the bactericidal activity of lactoferrin-derived peptides and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the influence on virulence factors of P. aeruginosa. METHODS AND RESULTS:Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides isolated from the N-terminal region of lactoferrin (LF) by pepsin digestion. In this study, we designed LFchimera containing LFcin amino acids 17-30 and LFampin amino acids 268-284. Pseudomonas aeruginosa cells were incubated in medium with peptides at different concentrations, and then the assays of viability, pyocyanin, elastase activity and biofilm formation of P. aeruginosa were performed. We found that the concentration-dependent antibactericidal activity and down-regulating pyocyanin, elastase and biofilm formation of LFchimera were significantly stronger than those of LF, LFcin, LFampin or LFcin plus LFampin. CONCLUSIONS:Our results indicated that LF, LFcin, LFampin and LFchimera were potential candidates to combat P. aeruginosa, and LFchimera was the most effective in them. SIGNIFICANCE AND IMPACT OF THE STUDY:The new LFchimera has better activity against P. aeruginosa than LF, LFcin and LFampin and may be a promising new compound for treatment of P. aeruginosa infection.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Xu G,Xiong W,Hu Q,Zuo P,Shao B,Lan F,Lu X,Xu Y,Xiong Sdoi
10.1111/j.1365-2672.2010.04751.xsubject
Has Abstractpub_date
2010-10-01 00:00:00pages
1311-8issue
4eissn
1364-5072issn
1365-2672pii
JAM4751journal_volume
109pub_type
杂志文章abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01727.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02159.x
更新日期:2004-01-01 00:00:00
abstract:AIM:To determine the sensitivity of methods for detection of injured and uninjured Escherichia coli O157:H7 (E. coli O157) in raw and pasteurized milk. METHODS AND RESULTS:Raw milk, pasteurized milk with 1.5% fat content and pasteurized milk with 3.5% fat content were spiked with E. coli O157 at low levels. The sample...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01646.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Growth of the lactic acid bacterium (LAB) Oenococcus oeni, which is involved in malolactic fermentation during the winemaking process, is stimulated by peptides originating from yeast. In this study, we investigated the impact of peptides on O. oeni growth, peptidase activity and the expression of genes encoding t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04032.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:The aims of this article were to select fungal species with high tolerance and high growth rate in mediums supplemented with limonene and citrus essential oils (CEOs), and to test the bioconversion capability of the chosen isolates for the bioproduction of aroma compounds. METHODS AND RESULTS:Based on the use of ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14777
更新日期:2021-01-01 00:00:00
abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13021
更新日期:2016-02-01 00:00:00
abstract:AIMS:The purpose of this study was to investigate the inactivation kinetics of Staphylococcus aureus in a ham model system by high hydrostatic pressure at ambient (25 degrees C) and selected temperatures (45, 55 degrees C). Selective [Baird Parker (BP) agar] and nonselective [brain heart infusion (BHI) agar] growth med...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03698.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:To identify changes in the bacterial community, at the phylum level brought about by varied crop management. METHODS AND RESULTS:Next-generation sequencing methods were used to compare the taxonomic structure of the bacterial community within 24 agricultural soils managed with either organic or conventional metho...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12822
更新日期:2015-07-01 00:00:00
abstract:AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14326
更新日期:2019-08-01 00:00:00
abstract:AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04591.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01590.x
更新日期:2002-01-01 00:00:00
abstract:AIM:Public health promotion of fruit and vegetables as healthier sources of nutrition in Nigeria has led to an increase in consumption of fruit and vegetables, particularly in the form of ready-to-eat (RTE) salads. However, the continual association of drug-resistant bacteria with fruit and vegetables creates an additi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14693
更新日期:2020-11-01 00:00:00
abstract:AIMS:To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization. METHODS AND RESULTS:A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01263.x
更新日期:2001-03-01 00:00:00
abstract:AIMS:The study established the inactivation kinetic parameters of an Acanthamoeba cyst isolate subjected to heating and chlorination. METHODS AND RESULTS:A strain of Acanthamoeba was isolated and purified from an area surrounding a pilot food plant. Mature cysts (14 days) were subjected to heat inactivation studies at...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14600
更新日期:2020-08-01 00:00:00
abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04326.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14190
更新日期:2019-04-01 00:00:00
abstract:AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12796
更新日期:2015-06-01 00:00:00
abstract:AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03386.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:Environmental contamination plays an important role in the transmission of infections, especially healthcare-associated infections. Disinfection transiently reduces contamination, but surfaces can rapidly become re-contaminated. Antimicrobial surfaces may partially overcome that limitation. The antimicrobial activ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12308
更新日期:2013-11-01 00:00:00
abstract:AIMS:Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12992
更新日期:2016-01-01 00:00:00
abstract:AIMS:Evaluation of the efficiency of peracetic acid in the disinfection of wastewater in a large treatment plant. METHODS AND RESULTS:Over a period of 18 months 30 sample collections were made, each consisting of three samples taken from: raw incoming sewage, secondary effluent (after 10-12 h) and secondary effluent d...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01451.x
更新日期:2001-11-01 00:00:00
abstract::Probiotics are increasingly being added to food in order to develop products with health-promoting properties. Particularly, Saccharomyces cereviceae var. boulardii yeast is recently being investigated like a starting-culture for development of functional and probiotic foods. Although the literature is abundant on the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14037
更新日期:2018-10-01 00:00:00
abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12960
更新日期:2015-12-01 00:00:00
abstract:AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02055.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS:The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13290
更新日期:2016-12-01 00:00:00
abstract:AIMS:To investigate the biofilm inhibitory activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. METHODS AND RESULTS:The pulp of red pitahaya and the leaves of red spinach were extracted using methanol fo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14091
更新日期:2019-01-01 00:00:00
abstract:AIMS: To investigate the prevalence and temporal patterns of antimicrobial resistance in wild rodents with no apparent exposure to antimicrobials. METHODS AND RESULTS: Two sympatric populations of bank voles and wood mice were trapped and individually monitored over a 2- year period for faecal carriage of antimicrob...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.04952.x
更新日期:2011-04-01 00:00:00
abstract:AIMS:Nutritional requirements of Lactobacillus fermentum Ogi E1 were studied in order to define a simplified fermentation medium. METHODS AND RESULTS:When grown with MRS-medium in 2l bioreactors, a biphasic pattern of growth and metabolite production was observed. Study of nutritional requirements resulted in a simpli...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01272.x
更新日期:2001-04-01 00:00:00
abstract:AIMS:To describe the occurrence and virulence gene pattern of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) in healthy goats of Jammu and Kashmir, India. METHODS AND RESULTS:A total of 220 E. coli strains belonging to 60 different 'O' serogroups was isolated from 206 local (nonmigra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02759.x
更新日期:2006-01-01 00:00:00
abstract:AIM:The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RE...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03756.x
更新日期:2008-08-01 00:00:00