当前位置： SCI文献检索 > JOURNAL OF APPLIED MICROBIOLOGY期刊下所有文献 > Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa.

Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa.

Abstract：

AIM:The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RESULTS:Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25.5% of water, 21.7% of vegetables as well as 56.5% and 43.5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10.3%, 8.6% and 7.8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliC(H7), rfbE(O157) and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12.2% and 8.8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. CONCLUSIONS:Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. SIGNIFICANCE AND IMPACT OF THE STUDY:Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.

journal_name

J Appl Microbiol

journal_title

Journal of applied microbiology

authors

Abong'o BO,Momba MN

doi

10.1111/j.1365-2672.2008.03756.x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

424-31

issue

2

eissn

1364-5072

issn

1365-2672

pii

JAM3756

journal_volume

105

pub_type

杂志文章

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