Development of multiplex real-time PCR for simultaneous detection of three Potyviruses in tobacco plants.

abstract：AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...

journal_title：Journal of applied microbiology

authors： Pritchard MF,Jack AA,Powell LC,Sadh H,Rye PD,Hill KE,Thomas DW

更新日期：2017-09-01 00:00:00

abstract：AIMS:To develop a method that is able to determine the microbial reduction in different dishwasher cleaning cycles and differentiate between different program parameters used. METHODS AND RESULTS:Stainless steel biomonitors were contaminated with Micrococcus luteus or Entereococcus faecium and cleaned in a specially p...

journal_title：Journal of applied microbiology

authors： Brands B,Schulze Struchtrup S,Stamminger R,Bockmühl DP

更新日期：2020-05-01 00:00:00

abstract：AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...

journal_title：Journal of applied microbiology

authors： Horakova K,Mlejnkova H,Mlejnek P

更新日期：2008-10-01 00:00:00

abstract：AIMS:To investigate the bactericidal activity of lactoferrin-derived peptides and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the influence on virulence factors of P. aeruginosa. METHODS AND RESULTS:Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides isolated fr...

journal_title：Journal of applied microbiology

authors： Xu G,Xiong W,Hu Q,Zuo P,Shao B,Lan F,Lu X,Xu Y,Xiong S

更新日期：2010-10-01 00:00:00

abstract：:A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...

journal_title：Journal of applied microbiology

authors： Hartikainen T,Ruuskanen J,Räty K,Von Wright A,Martikainen PJ

更新日期：2000-10-01 00:00:00

abstract：AIMS:To investigate the influence of micro-organisms associated with copper corrosion on 'blue water' corrosion in drinking water. METHODS AND RESULTS:Laboratory rigs comprising of polycarbonate containers attached to annealed copper plumbing tubes were filled with Melbourne drinking water and sterilized by autoclavin...

journal_title：Journal of applied microbiology

authors： Critchley MM,Pasetto R,O'Halloran RJ

更新日期：2004-01-01 00:00:00

abstract：:Recent advances in DNA sequencing technology have made it possible to elucidate the entire genomes of pathogenic bacteria, and advancements in bioinformatic tools have driven comparative studies of these genome sequences. These evaluations are dramatically increasing our ability to make valid considerations of the lim...

journal_title：Journal of applied microbiology

authors： McDevitt D,Payne DJ,Holmes DJ,Rosenberg M

更新日期：2002-01-01 00:00:00

abstract：AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...

journal_title：Journal of applied microbiology

authors： Hellström A,Qvirist L,Svanberg U,Veide Vilg J,Andlid T

更新日期：2015-05-01 00:00:00

abstract：AIMS:To investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. METHODS AND RESULTS:Laboratory marinated pork meat and commercial products from three Norwegian producers were examined. Lactic acid bacteria dominated in all products at t...

journal_title：Journal of applied microbiology

authors： Schirmer BC,Heir E,Langsrud S

更新日期：2009-06-01 00:00:00

abstract：AIMS:Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic pep...

journal_title：Journal of applied microbiology

authors： Subh L,Correa W,Pinkvos TJ,Behrens P,Brandenburg K,Gutsmann T,Stiesch M,Doll K,Winkel A

更新日期：2020-11-01 00:00:00

abstract：AIM:To develop a novel validated method for the isolation of Bifidobacterium animalis ssp. lactis BB-12 (BB-12) from faecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. METHODS AND RESULTS:A novel method for isolating and ...

journal_title：Journal of applied microbiology

authors： Poutsiaka DD,Mahoney IJ,McDermott LA,Stern LL,Thorpe CM,Kane AV,Baez-Giangreco C,McKinney J,Davidson LE,Leyva R,Goldin B,Snydman DR

更新日期：2017-05-01 00:00:00

abstract：AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...

journal_title：Journal of applied microbiology

authors： Milesi MM,McSweeney PL,Hynes ER

更新日期：2008-09-01 00:00:00

abstract：AIM:To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. METHODS AND RESULTS:Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method ta...

journal_title：Journal of applied microbiology

authors： Weidhaas JL,Macbeth TW,Olsen RL,Sadowsky MJ,Norat D,Harwood VJ

更新日期：2010-07-01 00:00:00

abstract：AIMS:To investigate the nitrogen-microbial community dynamics during composting of a mixture of nitrifying waste activated sludge (WAS) and fine organic fraction of residual household waste (RHW). To examine whether the addition of nitrifying sludge could promote ammonia oxidation and reduce ammonia emissions. METHODS...

journal_title：Journal of applied microbiology

authors： Zeng Y,Dabert P,Le Roux S,Mognol J,De Macedo FJ,De Guardia A

更新日期：2014-12-01 00:00:00

abstract：:Cancer is a prominent cause of morbidity and mortality worldwide, in spite of advances in therapeutic interventions and supportive care. In 2018 alone, there were 18·1 million new cancer cases and 9·6 million deaths indicating the need for novel anticancer agents. Plant-based products have often been linked with prote...

journal_title：Journal of applied microbiology

authors： Soopramanien M,Khan NA,Siddiqui R

更新日期：2020-12-23 00:00:00

abstract：AIMS:To identify changes in the bacterial community, at the phylum level brought about by varied crop management. METHODS AND RESULTS:Next-generation sequencing methods were used to compare the taxonomic structure of the bacterial community within 24 agricultural soils managed with either organic or conventional metho...

journal_title：Journal of applied microbiology

authors： Orr CH,Stewart CJ,Leifert C,Cooper JM,Cummings SP

更新日期：2015-07-01 00:00:00

abstract：AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...

journal_title：Journal of applied microbiology

authors： Montanari G,Borsari A,Chiavari C,Ferri G,Zambonelli C,Grazia L

更新日期：2004-01-01 00:00:00

abstract：AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

journal_title：Journal of applied microbiology

authors： Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

更新日期：2010-06-01 00:00:00

abstract：:Heterotrophic plate count (HPC) bacteria are naturally present in all aqueous environments. These bacteria undergo multiplication cycles in drinking water, especially in closed containers (bottled water) or in tap water when chlorine levels are dissipated, such as in dead ends in water mains or household plumbing. A s...

journal_title：Journal of applied microbiology

authors： Edberg SC,Kops S,Kontnick C,Escarzaga M

更新日期：1997-04-01 00:00:00

abstract：AIMS:To repurpose a silver-based antimicrobial textile coating product (Micro-Fresh 1911) as a dual-function antimicrobial laundry additive and textile coating. METHODS AND RESULTS:Survival of Escherichia coli or Staphylococcus aureus type and clinical isolates in a domestic 40°C wash was assessed with and without soi...

journal_title：Journal of applied microbiology

authors： Owen L,Laird K

更新日期：2020-09-11 00:00:00

abstract：AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...

journal_title：Journal of applied microbiology

authors： Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

更新日期：2010-04-01 00:00:00

abstract：:Avoparcin was used as a feed additive in Norwegian broiler and turkey production from 1986 until 1995. It was banned due to the selection of VanA-type vancomycin-resistant enterococci (VRE) in animal husbandry and to reduce the potential for human exposure to VRE. The aim of the present study was to investigate the pr...

journal_title：Journal of applied microbiology

authors： Borgen K,Simonsen GS,Sundsfjord A,Wasteson Y,Olsvik O,Kruse H

更新日期：2000-09-01 00:00:00

abstract：AIMS:To employ an in vitro screening regime to select a probiotic Bifidobacterium strain to complement resistant starch (Hi-maizetrade mark) in a synbiotic yoghurt. METHODS AND RESULTS:Of 40 Bifidobacterium isolates examined, only B. lactis Laftitrade mark B94 possessed all of the required characteristics. This isolat...

journal_title：Journal of applied microbiology

authors： Crittenden RG,Morris LF,Harvey ML,Tran LT,Mitchell HL,Playne MJ

更新日期：2001-02-01 00:00:00

abstract：AIMS:To calculate fermentation efficiency in a continuous ethanol production process, we aimed to develop a robust mathematical method based on the analysis of metabolic by-product formation. METHODS AND RESULTS:This method is in contrast to the traditional way of calculating ethanol fermentation efficiency, where the...

journal_title：Journal of applied microbiology

authors： Canseco Grellet MA,Castagnaro A,Dantur KI,De Boeck G,Ahmed PM,Cárdenas GJ,Welin B,Ruiz RM

更新日期：2016-10-01 00:00:00

abstract：AIMS:To assess the biodiversity of a large number of microbial fuel cell (MFC) anodes from a variety of MFC designs, all enriched with domestic wastewater, using a molecular fingerprinting method. METHODS AND RESULTS:We optimized a protocol allowing the rapid characterization of MFC communities using terminal restrict...

journal_title：Journal of applied microbiology

authors： Lefebvre O,Nguyen TT,Al-Mamun A,Chang IS,Ng HY

更新日期：2010-09-01 00:00:00

abstract：AIMS:Cucumber angular leaf spot caused by Pseudomonas syringae pv. lachrymans (Psl) is an important and destructive disease worldwide, and no effective technique has been developed for the control of the pathogen. Detection of infection or latent in cucumber plants is critical to evaluate disease progress and strengthe...

journal_title：Journal of applied microbiology

authors： Meng XL,Xie XW,Shi YX,Chai AL,Ma ZH,Li BJ

更新日期：2017-02-01 00:00:00

abstract：:We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemilumine...

journal_title：Journal of applied microbiology

authors： Venkitanarayanan KS,Faustman C,Crivello JF,Khan MI,Hoagland TA,Berry BW

更新日期：1997-03-01 00:00:00

abstract：AIM:Gonorrhoea remains a leading public health burden and the development of vaccine against gonorrhoea becomes more urgent. Here, a novel Neisseria gonorrhoeae DNA vaccine delivered by Salmonella enteritidis ghosts was developed and the immune responses of the vaccine candidate were evaluated. METHODS AND RESULTS:Nei...

journal_title：Journal of applied microbiology

authors： Jiao H,Yang H,Zheng W,Zhang Q,Zhao D,Li G

更新日期：2020-08-08 00:00:00

abstract：AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...

journal_title：Journal of applied microbiology

authors： Coulon J,Perello MC,Lonvaud-Funel A,de Revel G,Renouf V

更新日期：2010-04-01 00:00:00

abstract：AIM:To develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. METHODS AND RESULTS:Urea was added to faecal matter (17% dry matter) at concentrations of 0.5-2% (w/w) and inactivation of Salmonella enterica subspecies 1 serovar Typhimurium (Salm. Typhimur...

journal_title：Journal of applied microbiology

authors： Nordin A,Ottoson JR,Vinnerås B

更新日期：2009-11-01 00:00:00