Rapid estimation of spoilage bacterial load in aerobically stored meat by a quantitative polymerase chain reaction.

Abstract:

:We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemiluminescence revealed that the concentration of the amplified product was dependent on cycle number and the initial number of bacteria present in the sample. Statistical analysis of the results indicated a correlation coefficient of 0.94 (P < 0.001) between aerobic plate count and QPCR luminosity units.

journal_name

J Appl Microbiol

authors

Venkitanarayanan KS,Faustman C,Crivello JF,Khan MI,Hoagland TA,Berry BW

doi

10.1046/j.1365-2672.1997.00368.x

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

359-64

issue

3

eissn

1364-5072

issn

1365-2672

journal_volume

82

pub_type

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