Abstract:
:We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemiluminescence revealed that the concentration of the amplified product was dependent on cycle number and the initial number of bacteria present in the sample. Statistical analysis of the results indicated a correlation coefficient of 0.94 (P < 0.001) between aerobic plate count and QPCR luminosity units.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Venkitanarayanan KS,Faustman C,Crivello JF,Khan MI,Hoagland TA,Berry BWdoi
10.1046/j.1365-2672.1997.00368.xsubject
Has Abstractpub_date
1997-03-01 00:00:00pages
359-64issue
3eissn
1364-5072issn
1365-2672journal_volume
82pub_type
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journal_title:Journal of applied microbiology
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更新日期:2007-12-01 00:00:00
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更新日期:2018-03-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2017-11-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2014-08-01 00:00:00