Abstract：
AIMS:To isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in Cuba without chemical fertilizers. METHODS AND RESULTS:Two N2-fixing isolates, 9C and T2, were obtained from surface-sterilized stems and roots, respectively, of sugarcane variety ML3-18. Both isolates showed acetylene reduction and H2 production in nitrogen-free media. Nitrogenase activity measured by H2 production was about 15 times higher for isolate 9C than for T2 or for Gluconoacetobacter diazotrophicus (PAL-5 standard strain, ATCC 49037). The nifH gene segment was amplified from both isolates using specific primers. Classification of both T2 and 9C was made on the basis of morphological, biochemical, PCR tests and 16S rDNA sequence analysis. CONCLUSIONS:Isolate 9C was identified as a Pantoea species from its 16S rDNA, but showed considerable differences in physiological properties from previously reported species of this genus. For example, 9C can be cultured over a wide range of temperature, pH and salt concentration, and showed high H2 production (up to 67.7 nmol H2 h(-1) 10(10) cell(-1)). Isolate T2 was a strain of Gluconacetobacter diazotrophicus. SIGNIFICANCE AND IMPACT OF THE STUDY:A new N2-fixing endophyte, i.e. Pantoea, able to produce H2 and to grow in a wide range of conditions, was isolated from sugarcane stem tissue and characterized. The strain with these attributes may well be valuable for agriculture.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Loiret FG,Ortega E,Kleiner D,Ortega-Rodés P,Rodés R,Dong Zdoi
10.1111/j.1365-2672.2004.02329.xsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
504-11issue
3eissn
1364-5072issn
1365-2672pii
JAM2329journal_volume
97pub_type
杂志文章abstract：AIMS:To determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (LAB). To know if a similar freeze-drying protocol can be used for both micro-organisms. METHODS AND RESULTS:Cells from liquid culture media were recovered and concentrated...
journal_title：Journal of applied microbiology
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更新日期：2017-06-01 00:00:00
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journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04092.x
更新日期：2009-04-01 00:00:00
abstract：AIMS:The metabolism of phenanthrene and anthracene by a moderate thermophilic Nocardia otitidiscaviarum strain TSH1 was examined. METHODS AND RESULTS:When strain TSH1 was grown in the presence of anthracene, four metabolites were identified as 1,2-dihydroxy-1,2-dihydroanthracene, 3-(2-carboxyvinyl)naphthalene-2-carbox...
journal_title：Journal of applied microbiology
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doi：10.1111/j.1365-2672.2008.03753.x
更新日期：2008-08-01 00:00:00
abstract：AIMS:This study was applied to evaluate the usefulness of a high-throughput sample preparation protocol prior to the application of quantitative real-time PCR (qPCR) for the early diagnosis of bloodstream and pyogenic infections in humans and animals compared to matrix-assisted laser desorption ionization-time of fligh...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
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更新日期：2020-10-18 00:00:00
abstract：AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03813.x
更新日期：2008-09-01 00:00:00
abstract：AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04280.x
更新日期：2009-09-01 00:00:00
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journal_title：Journal of applied microbiology
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更新日期：2017-01-01 00:00:00
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journal_title：Journal of applied microbiology
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更新日期：2017-03-01 00:00:00
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journal_title：Journal of applied microbiology
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更新日期：2010-12-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03849.x
更新日期：2008-10-01 00:00:00
abstract：AIM:Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts was studied with different compost types, storage conditions and inoculum levels. METHODS AND RESULTS:A cocktail of three E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or p...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13719
更新日期：2018-05-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03041.x
更新日期：2007-01-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01680.x
更新日期：2002-01-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02218.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To evaluate the prevalence of Cryptosporidium and Giardia in surface water supplies from the province of Alava, northern Spain, and to investigate possible associations among the presence of these pathogenic protozoa with microbiological, physicochemical and atmospheric parameters. METHODS AND RESULTS:A total of ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03193.x
更新日期：2007-03-01 00:00:00
abstract：AIMS:To compare clinical and environmental Aspergillus fumigatus strains on their toxicogenic and immunosuppressive capacity. METHODS AND RESULTS:A total of 51 strains of A. fumigatus isolated from clinical and corn silage samples were assayed. All A. fumigatus strains were assayed for gliotoxin production, therefore ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12673
更新日期：2015-01-01 00:00:00
abstract：AIMS:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cyto...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02496.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:Development of a real-time PCR method for the specific detection of Salmonella Dublin. METHODS AND RESULTS:The method was directed towards a Salm. Dublin-specific sequence of the vagC gene on the Salmonella virulence plasmid (pSDV) and towards Salmonella genus-specific sequence of the invA gene, serving as an int...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05378.x
更新日期：2012-09-01 00:00:00
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journal_title：Journal of applied microbiology
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更新日期：1999-11-01 00:00:00
abstract：:Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes fr...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：AIMS:We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab sampling procedure. METHODS AND RESULTS:Bacillus anthracis Sterne or Bacillu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01651.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. METHODS AND RESULTS:This micro-organism, on t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03568.x
更新日期：2008-02-01 00:00:00
abstract：AIMS:To identify active phenolic constituents in muscadine grape skin (MGS) extracts and determine interactions among compounds while further exploring their anti-Helicobacter pylori potential in vitro. METHODS AND RESULTS:The inhibitory effects of quercetin and resveratrol, active polyphenols identified in MGS extrac...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12129
更新日期：2013-04-01 00:00:00
abstract：AIMS:The goal of this research was the development of a PCR-based assay to identify important decay fungi from wood of hardwood tree species in northern temperate regions. METHODS AND RESULTS:Eleven taxon-specific primers were designed for PCR amplification of either nuclear or mitochondrial ribosomal DNA regions of A...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03378.x
更新日期：2007-11-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.04952.x
更新日期：2011-04-01 00:00:00
abstract：AIMS:Fungal infection is still a life-threatening risk for the immunocompromised population such as AIDS patients and those who receive treatments with immunosuppressors and/or frequent administrations of wide-spectrum antibiotics, which inevitably lead to the drug resistance and unbalanced microflora populations. The ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2005.02760.x
更新日期：2006-01-01 00:00:00
abstract：AIMS:The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. METHODS AND RESULTS:Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04726.x
更新日期：2010-09-01 00:00:00
abstract：AIMS:The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated. METHODS AND RESULTS:Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions (Eh7: +200 mV and -400 mV) using nitrogen and hydrogen as reducing agents. Growth wa...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01832.x
更新日期：2003-01-01 00:00:00
