A new real-time PCR method for the identification of Salmonella Dublin.

abstract：AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...

journal_title：Journal of applied microbiology

authors： Romanov MN,Bato RV,Yokoyama MT,Rust SR

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

journal_title：Journal of applied microbiology

authors： Wang L,Li Y,Mustapha A

更新日期：2009-11-01 00:00:00

abstract：AIMS:The ComPath project is a pan-European programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (MIC) determination. Here, the susceptibility of major pathogens is reported from antimicr...

journal_title：Journal of applied microbiology

authors： Ludwig C,de Jong A,Moyaert H,El Garch F,Janes R,Klein U,Morrissey I,Thiry J,Youala M

更新日期：2016-11-01 00:00:00

abstract：AIMS:Campylobacter contamination in French chicken production from the farm to the consumer was determined using a PCR assay for bacteria detection and identification. METHODS AND RESULTS:Samples were bird droppings from poultry houses, neck skins, livers, hearts, gizzards, wings, legs and escalopes from slaughterhous...

journal_title：Journal of applied microbiology

authors： Denis M,Refrégier-Petton J,Laisney MJ,Ermel G,Salvat G

更新日期：2001-08-01 00:00:00

abstract：AIMS:To evaluate beneficial effect of two food additives, ammonium molybdate (NH4-Mo) and sodium bicarbonate (NaBi), on antagonistic yeasts for control of brown rot caused by Monilinia fructicola in sweet cherry fruit under various storage conditions. The mechanisms of action by which food additives enhance the efficac...

journal_title：Journal of applied microbiology

authors： Qin GZ,Tian SP,Xu Y,Chan ZL,Li BQ

更新日期：2006-03-01 00:00:00

abstract：AIMS:This study investigates changes in microbiological and physicochemical parameters during large-scale, thermophilic composting of a single batch of municipal organic waste. The inter-relationships between the microbial biomass and community structure as well as several physicochemical parameters and estimates of ma...

journal_title：Journal of applied microbiology

authors： Steger K,Sjögren AM,Jarvis A,Jansson JK,Sundh I

更新日期：2007-08-01 00:00:00

abstract：:The genus Lactococcus comprises 12 species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium, probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species...

journal_title：Journal of applied microbiology

authors： Saraoui T,Leroi F,Björkroth J,Pilet MF

更新日期：2016-10-01 00:00:00

abstract：AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences...

journal_title：Journal of applied microbiology

authors： Botti S,Bertaccini A

更新日期：2003-01-01 00:00:00

abstract：AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...

journal_title：Journal of applied microbiology

authors： Pintado J,Guyot JP,Ampe F

更新日期：2003-01-01 00:00:00

abstract：:The exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in Lysobacter, 11 new species having been described since 2005, and in Stenotrophomonas with six new species since 2000. The new species of Lysobacter, isol...

journal_title：Journal of applied microbiology

authors： Hayward AC,Fegan N,Fegan M,Stirling GR

更新日期：2010-03-01 00:00:00

abstract：:The effect of divalent cations on the inactivation of Escherichia coli by high hydrostatic pressure was investigated. The presence of 0.5 mmol l-1 of CaCl2, MgCl2, MnCl2 and FeCl2 reduced pressure inactivation of E. coli MG1655, while 0.5 mmol l-1 of ZnCl2, NiCl2, CuCl2 and CoCl2 increased inactivation. Baroprotection...

journal_title：Journal of applied microbiology

authors： Hauben KJ,Bernaerts K,Michiels CW

更新日期：1998-10-01 00:00:00

abstract：AIMS:To investigate the effect of silver-based antimicrobial material incorporated in the inner liners of refrigerators on food safety and quality. METHODS AND RESULTS:In the first stage, the bactericidal effect was tested in the laboratory. Silver-containing samples and control plates were inoculated with different b...

journal_title：Journal of applied microbiology

authors： Kampmann Y,De Clerck E,Kohn S,Patchala DK,Langerock R,Kreyenschmidt J

更新日期：2008-06-01 00:00:00

abstract：AIMS:Silage is grass, preserved by fermentation and used as winter feed for cattle. The impact of a range of current grass silage preparation practices on the survival of Escherichia coli C600φ3538(Δvtx2 ::cat) and on the induction, release and infectivity of free phage were investigated. METHODS AND RESULTS:Wilted an...

journal_title：Journal of applied microbiology

authors： Nyambe S,Burgess C,Whyte P,O'Kiely P,Bolton D

更新日期：2017-05-01 00:00:00

abstract：AIMS:One of the major limitations to the production of alfalfa (Medicago sativa) is the fungus Phoma medicaginis, which infects alfalfa and causes leaf spots. This study aims to understand alfalfa's response to P. medicaginis infection, the colonization of arbuscular mycorrhizal fungus (AMF) and the effect of AMF on pl...

journal_title：Journal of applied microbiology

authors： Li Y,Duan T,Nan Z,Li Y

更新日期：2019-07-16 00:00:00

abstract：AIMS:The aims of the study were to quantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and to determine the vulnerability to aflatoxin increases after purchase. METHODS AND RESULTS:Aflatoxin concentrations were determin...

journal_title：Journal of applied microbiology

authors： Kachapulula PW,Akello J,Bandyopadhyay R,Cotty PJ

更新日期：2017-06-01 00:00:00

abstract：AIM:To describe the relationship between the concentration of different indicator bacteria in red meat. METHODS AND RESULTS:Enumeration data for aerobic plate count (APC), Enterobacteriaceae, coliforms and Escherichia coli biotype I were analysed from an Australia-wide survey of beef carcasses, sheep carcasses, frozen...

journal_title：Journal of applied microbiology

authors： Jordan D,Phillips D,Sumner J,Morris S,Jenson I

更新日期：2007-01-01 00:00:00

abstract：AIMS:The cell-surface display of chitinase Chi9602ΔSP was constructed on Bacillus thuringiensis BMB171 by using two repeat N-terminal regions of autolysin (Mbgn)2 as the anchoring motif. This study aimed to prepare a chitinolytic whole-cell B. thuringiensis biocatalyst with improved catalytic and antifungal activities....

journal_title：Journal of applied microbiology

authors： Tang M,Sun X,Zhang S,Wan J,Li L,Ni H

更新日期：2017-01-01 00:00:00

abstract：:The inactivation of Bacillus subtilis spores by ultrasonic treatments under static pressure (Mano-Sonication, MS) and a combined MS/heat treatment (Mano-Thermo-Sonication) was investigated. The sporicidal effect of MS treatments depended on static pressure, amplitude of ultrasonic waves and treatment temperature. At 7...

journal_title：Journal of applied microbiology

authors： Raso J,Palop A,Pagán R,Condón S

更新日期：1998-11-01 00:00:00

abstract：AIMS:The purposes were to evaluate the detection of low levels of Campylobacter in water by dead-end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS:The DEUF technique followed by detection according to ISO 10272 was tested on artificially and natu...

journal_title：Journal of applied microbiology

authors： Ferrari S,Frosth S,Svensson L,Fernström LL,Skarin H,Hansson I

更新日期：2019-10-01 00:00:00

abstract：AIMS:Algae are favourable as a biofuel source because of the potential high oil content and fast generation of biomass. However, one of the challenges for this technology is achieving high oil content while maintaining exponential or high growth of the organism. Introducing a two-stage reactor to optimize both growth a...

journal_title：Journal of applied microbiology

authors： Csavina JL,Stuart BJ,Riefler RG,Vis ML

更新日期：2011-08-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...

journal_title：Journal of applied microbiology

authors： Cafaro C,Bonomo MG,Salzano G

更新日期：2014-01-01 00:00:00

abstract：AIMS:This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. METHODS AND RESULTS:The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when ...

journal_title：Journal of applied microbiology

authors： Wu Z,Xuanyuan Z,Li R,Jiang D,Li C,Xu H,Bai Y,Zhang X,Turakainen H,Saris PE,Savilahti H,Qiao M

更新日期：2009-01-01 00:00:00

abstract：AIM:To determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. METHODS:Putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm....

journal_title：Journal of applied microbiology

authors： Kagkli DM,Vancanneyt M,Vandamme P,Hill C,Cogan TM

更新日期：2007-11-01 00:00:00

abstract：:The combined effects of pH, water activity (aw), oxygen (O2) and carbon dioxide (CO2) levels on growth and sporulation of 10 common food-borne fungi were studied. The use of a multivariate statistical method (PLS) for the analysis of data showed that the fungi could be grouped according to their physiological response...

journal_title：Journal of applied microbiology

authors： Haasum I,Nielsen PV

更新日期：1998-03-01 00:00:00

abstract：:The rumen contents of red deer (Cervus elaphus) were used to isolate bacterial capable of fermenting glycerol. The biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. The isolate (DR3) was tentatively identified as a strain of the species Klebsiella planticola as based on phenotyp...

journal_title：Journal of applied microbiology

authors： Jarvis GN,Moore ER,Thiele JH

更新日期：1997-08-01 00:00:00

abstract：AIMS:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. METHODS AND RESULTS:Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain vi...

journal_title：Journal of applied microbiology

authors： Fernandes T,Carvalho BF,Mantovani HC,Schwan RF,Ávila CLS

更新日期：2019-09-01 00:00:00

abstract：:Two abundant surface proteins, EA1 and Sap, are components of the Bacillus anthracis surface layer (S-layer). Their corresponding genes have been cloned, shown to be clustered on the chromosome and sequenced. EA1 and Sap each possess three 'S-layer homology' motifs. Single and double disrupted mutants were constructed...

journal_title：Journal of applied microbiology

authors： Fouet A,Mesnage S,Tosi-Couture E,Gounon P,Mock M

更新日期：1999-08-01 00:00:00

abstract：AIMS:To study biomineralization of Monocrotophos (MCP) and identify the metabolites formed during biodegradation. METHODS AND RESULTS:Two cultures, namely Arthrobacter atrocyaneus MCM B-425 and Bacillus megaterium MCM B-423, were isolated by enrichment and adaptation culture technique from soil exposed to MCP. The iso...

journal_title：Journal of applied microbiology

authors： Bhadbhade BJ,Sarnaik SS,Kanekar PP

更新日期：2002-01-01 00:00:00

abstract：AIMS:The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species A-D targeting the 5' untranslated region (5' UTR) of enteroviruses genome. METHODS AND RESULTS:The RNA was converted to cDNA by the reverse transcriptase and then amplified via LAMP by the...

journal_title：Journal of applied microbiology

authors： Daskou M,Dimitriou TG,Alexopoulou DS,Tsakogiannis D,Amoutzias GD,Mossialos D,Kyriakopoulou Z,Markoulatos P

更新日期：2021-01-01 00:00:00

abstract：AIMS:Cucumber angular leaf spot caused by Pseudomonas syringae pv. lachrymans (Psl) is an important and destructive disease worldwide, and no effective technique has been developed for the control of the pathogen. Detection of infection or latent in cucumber plants is critical to evaluate disease progress and strengthe...

journal_title：Journal of applied microbiology

authors： Meng XL,Xie XW,Shi YX,Chai AL,Ma ZH,Li BJ

更新日期：2017-02-01 00:00:00