Real-time PCR versus MALDI-TOF MS and culture-based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals.

abstract：AIMS:To investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. METHODS AND RESULTS:Laboratory marinated pork meat and commercial products from three Norwegian producers were examined. Lactic acid bacteria dominated in all products at t...

journal_title：Journal of applied microbiology

authors： Schirmer BC,Heir E,Langsrud S

更新日期：2009-06-01 00:00:00

abstract：AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of ...

journal_title：Journal of applied microbiology

authors： Bernal-Mercado AT,Gutierrez-Pacheco MM,Encinas-Basurto D,Mata-Haro V,Lopez-Zavala AA,Islas-Osuna MA,Gonzalez-Aguilar GA,Ayala-Zavala JF

更新日期：2020-02-01 00:00:00

abstract：AIM:To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. METHODS AND RESULTS:Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method ta...

journal_title：Journal of applied microbiology

authors： Weidhaas JL,Macbeth TW,Olsen RL,Sadowsky MJ,Norat D,Harwood VJ

更新日期：2010-07-01 00:00:00

abstract：AIM:Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch-blood-egg yolk-polymyxin B-trimethoprim-ceftazidime (SBYPTC) agar was established to isolate and specify the number of Bacillus cereus in food products. METHODS AND RESULTS:The effectiveness of ...

journal_title：Journal of applied microbiology

authors： Talahmeh N,Abu-Rumeileh S,Al-Razem F

更新日期：2020-05-01 00:00:00

abstract：AIMS:To assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of Satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline...

journal_title：Journal of applied microbiology

authors： Chorianopoulos NG,Giaouris ED,Skandamis PN,Haroutounian SA,Nychas GJ

更新日期：2008-06-01 00:00:00

abstract：AIMS:To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS:The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated...

journal_title：Journal of applied microbiology

authors： Krusong W,Kerdpiboon S,Pornpukdeewattana S,Jindaprasert A

更新日期：2016-12-01 00:00:00

abstract：AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...

journal_title：Journal of applied microbiology

authors： Pathirana E,McPherson A,Whittington R,Hick P

更新日期：2019-08-01 00:00:00

abstract：AIMS:To isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in Cuba without chemical fertilizers. METHODS AND RESULTS:Two N2-fixing isolates, 9C and T2, were obtained from surface-sterilized stems and roots, respectively, of sugarcane variety ML3-18. Both isolates showed acetylene reduction a...

journal_title：Journal of applied microbiology

authors： Loiret FG,Ortega E,Kleiner D,Ortega-Rodés P,Rodés R,Dong Z

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...

journal_title：Journal of applied microbiology

authors： Kairyte K,Lapinskas S,Gudelis V,Luksiene Z

更新日期：2012-06-01 00:00:00

abstract：:Pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. Among the fungal diseases, blue mold caused by Penicillium expansum, grey mould caused by Botrytis cinerea, Mucor rot caused by Mucor piriformis are common on pear fruits. Other (weak) pathogens like Phi...

journal_title：Journal of applied microbiology

authors： Mari M,Bertolini P,Pratella GC

更新日期：2003-01-01 00:00:00

abstract：AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...

journal_title：Journal of applied microbiology

authors： Flores C,Casasanero R,Trejo-Hernández MR,Galindo E,Serrano-Carreón L

更新日期：2010-03-01 00:00:00

abstract：:Thirty-eight Lactococcus strains, isolated from raw milk produced in two dairy areas in Normandy, were identified at the phenotypic level. Only Lactococcus lactis strains with the lactis phenotype were found in the milk samples. Most strains fermented lactose (97%) and showed proteinase activity (76%). Isolates were c...

journal_title：Journal of applied microbiology

authors： Desmasures N,Mangin I,Corroler D,Guéguen M

更新日期：1998-12-01 00:00:00

abstract：:Grab samples of drinking water collected from reservoirs and from creeks flowing over pristine land, farmland or land having mixed use were analysed for their physicochemical and microbiological characteristics. A significant difference between sites for conductivity and sites for pH was noted using a two-way ANOVA. N...

journal_title：Journal of applied microbiology

authors： Thurman R,Faulkner B,Veal D,Cramer G,Meiklejohn M

更新日期：1998-04-01 00:00:00

abstract：AIMS:To investigate the biofilm inhibitory activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. METHODS AND RESULTS:The pulp of red pitahaya and the leaves of red spinach were extracted using methanol fo...

journal_title：Journal of applied microbiology

authors： Yong YY,Dykes G,Lee SM,Choo WS

更新日期：2019-01-01 00:00:00

abstract：AIMS:To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. METHODS AND RESULTS:A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic propertie...

journal_title：Journal of applied microbiology

authors： Miñana-Galbis D,Farfán M,Lorén JG,Fusté MC

更新日期：2002-01-01 00:00:00

abstract：AIMS:To evaluate a standard aerosolization method for uniformly depositing threat-representative spores onto surfaces. METHODS AND RESULTS:Lyophilized Bacillus anthracis ΔSterne spores, coated in silica, were aerosolized into a containment chamber and deposited onto nine surface types by two independent laboratories. ...

journal_title：Journal of applied microbiology

authors： Harnish D,Heimbuch BK,McDonald M,Kinney K,Dion M,Stote R,Rastogi V,Smith L,Wallace L,Lumley A,Schreuder-Gibson H,Wander JD

更新日期：2014-07-01 00:00:00

abstract：AIMS:To monitor seasonal variations in the microbiology of maize silage and to determine whether the risk of fungal spoilage varies during whole-year storage. METHODS AND RESULTS:A continuous survey of 20 maize silage stacks was conducted over a period from three to 11 months after ensiling. Filamentous fungi, yeasts ...

journal_title：Journal of applied microbiology

authors： Storm IM,Kristensen NB,Raun BM,Smedsgaard J,Thrane U

更新日期：2010-09-01 00:00:00

abstract：AIMS:To develop a method for studying the microbial spoilage of water-in-oil emulsions and to use this to investigate (i) the intrinsic stability of water-in-oil formulations and (ii) Pseudomonas aeruginosa SP1-induced spoilage of a proprietary emulsion. METHODS AND RESULTS:Aliquots of test emulsion were placed into w...

journal_title：Journal of applied microbiology

authors： O'May GA,Allison DG,Gilbert P

更新日期：2004-01-01 00:00:00

abstract：:The survival of Bacteroides thetaiotaomicron strain BTX under rumen-simulating conditions was studied. Strain BTX is a recombinant variant of strain 5482 engineered for the production of high levels of xylanase, an enzyme important in the degradation of hemicellulose. Strain BTX was not inhibited by compounds present ...

journal_title：Journal of applied microbiology

authors： Cotta MA,Whitehead TR,Rasmussen MA

更新日期：1997-06-01 00:00:00

abstract：AIMS:A number of previous studies have shown that plant extracts can inhibit formation of dental plaque. The ability of extracts of Rosmarinus officianalis L., Salvia officianalis L., unfermented cocoa, red grape seed and green tea to inhibit plaque bacteria, glucosyltransferase activity, glucan and plaque formation in...

journal_title：Journal of applied microbiology

authors： Smullen J,Finney M,Storey DM,Foster HA

更新日期：2012-10-01 00:00:00

abstract：AIMS:The environmental pathogen Streptococcus uberis causes intramammary infections in dairy cows. Because biofilm growth might contribute to Strep. uberis mastitis, we conducted a biological screen to identify genes potentially involved in the regulation of biofilm growth. METHODS AND RESULTS:By screening a transposo...

journal_title：Journal of applied microbiology

authors： Salomäki T,Karonen T,Siljamäki P,Savijoki K,Nyman TA,Varmanen P,Iivanainen A

更新日期：2015-01-01 00:00:00

abstract：AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

journal_title：Journal of applied microbiology

authors： Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

更新日期：2011-01-01 00:00:00

abstract：:An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective cultu...

journal_title：Journal of applied microbiology

authors： Brook MD,Currie B,Desmarchelier PM

更新日期：1997-05-01 00:00:00

abstract：AIMS:A batch chlorination system was optimized for on-site wastewater treatment and reuse system (OWRS) and its efficiency was evaluated for reducing viruses, protozoa, bacteria and antimicrobial resistance in cold and warm seasons. METHODS AND RESULTS:The OWRS performance in reducing microbial contaminants was determ...

journal_title：Journal of applied microbiology

authors： Park E,Mancl KM,Tuovinen OH,Bisesi MS,Lee J

更新日期：2016-12-01 00:00:00

abstract：AIMS:The pathogenic potential of Arcobacter butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays. METHODS AND RESULTS:Five of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells...

journal_title：Journal of applied microbiology

authors： Karadas G,Bücker R,Sharbati S,Schulzke JD,Alter T,Gölz G

更新日期：2016-01-01 00:00:00

abstract：AIMS:The minimum inhibitory concentration (MIC) of oregano essential oil (OEO) and two of its principle components, i.e. thymol and carvacrol, against Pseudomonas aeruginosa and Staphylococcus aureus was assessed by using an innovative technique. The mechanism of action of the above substances was also investigated. M...

journal_title：Journal of applied microbiology

authors： Lambert RJ,Skandamis PN,Coote PJ,Nychas GJ

更新日期：2001-09-01 00:00:00

abstract：AIMS:To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus (TEV), Potato virus Y (PVY) and Tobacco vein banding mosaic virus (TVBMV). METHODS AND RESULTS:Specific primer and probe combinations for TEV and TVBMV were developed from the coa...

journal_title：Journal of applied microbiology

authors： Dai J,Peng H,Chen W,Cheng J,Wu Y

更新日期：2013-02-01 00:00:00

abstract：AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...

journal_title：Journal of applied microbiology

authors： Zhang C,Yu L,Qian R

更新日期：2007-11-01 00:00:00

abstract：AIMS:To employ an in vitro screening regime to select a probiotic Bifidobacterium strain to complement resistant starch (Hi-maizetrade mark) in a synbiotic yoghurt. METHODS AND RESULTS:Of 40 Bifidobacterium isolates examined, only B. lactis Laftitrade mark B94 possessed all of the required characteristics. This isolat...

journal_title：Journal of applied microbiology

authors： Crittenden RG,Morris LF,Harvey ML,Tran LT,Mitchell HL,Playne MJ

更新日期：2001-02-01 00:00:00

abstract：AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed...

journal_title：Journal of applied microbiology

authors： Bonilla M,Olivaro C,Corona M,Vazquez A,Soubes M

更新日期：2005-01-01 00:00:00