A multiplex PCR-based method for the detection and early identification of wood rotting fungi in standing trees.

abstract：:Medical devices provide critical care and diagnostic applications through patient contact. Sterility assurance level (SAL) may be defined as the probability of a single viable micro-organism occurring on an item after a sterilization process. Sterilization microbiology often relies upon using an overkill validation me...

journal_title：Journal of applied microbiology

authors： McEvoy B,Lynch M,Rowan NJ

更新日期：2020-11-06 00:00:00

abstract：AIM:To evaluate the antifungal activity of extracts of Chamaecostus cuspidatus against Candida and Trichophyton species. METHODS AND RESULTS:Crude ethanol extracts of leaves, stems and rhizomes were prepared and evaluated for antimicrobial activity. Only the rhizomes extract (RE) showed antifungal activity but had no ...

journal_title：Journal of applied microbiology

authors： Barros Cota B,Batista Carneiro de Oliveira D,Carla Borges T,Cristina Catto A,Valverde Serafim C,Rogelis Aquiles Rodrigues A,Kohlhoff M,Leomar Zani C,Assunção Andrade A

更新日期：2021-01-01 00:00:00

abstract：AIMS:To determine conditions for generation and recovery of Bacillus subtilis spore populations heavily damaged by moist heat treatment. METHODS AND RESULTS:Bacillus subtilis spores were treated with moist heat and spore viability was assessed on different media. A rich medium and several minimal media gave similar sp...

journal_title：Journal of applied microbiology

authors： Coleman WH,Setlow P

更新日期：2009-05-01 00:00:00

abstract：AIMS:  To determine the capacity of secondary metabolite of strain SX-4, to enhance the nonspecific immunity and survival of carp (Cyprinus carpio), and to identify the constituents that are responsible. METHODS AND RESULTS:  A thermophilic strain SX-4 that is able to produce immunostimulatory metabolite was isolated ...

journal_title：Journal of applied microbiology

authors： Liu J,Lei Y,Wang F,Yi Y,Liu Y,Wang G

更新日期：2011-04-01 00:00:00

abstract：AIMS:To study the expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies (MAbs) C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. METHODS AND RESULTS:The reaction patterns of antibodies to L. monocytogenes held in stressful environments for a short...

journal_title：Journal of applied microbiology

authors： Geng T,Kim KP,Gomez R,Sherman DM,Bashir R,Ladisch MR,Bhunia AK

更新日期：2003-01-01 00:00:00

abstract：AIM:To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS:The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated...

journal_title：Journal of applied microbiology

authors： Ercan UK,Sen B,Brooks AD,Joshi SG

更新日期：2018-08-01 00:00:00

abstract：AIMS:The aim of this study was to develop a novel selective agar for the specific isolation and detection of Bacillus anthracis. METHODS AND RESULTS:Based on published data on antibiotic resistance and susceptibility of B. anthracis and other closely related species of the Bacillus cereus sensu lato group, a new selec...

journal_title：Journal of applied microbiology

authors： Rohde A,Papp S,Feige P,Grunow R,Kaspari O

更新日期：2020-08-01 00:00:00

abstract：AIMS:To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. METHODS AND RESULTS:Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combinati...

journal_title：Journal of applied microbiology

authors： Maré L,Wolfaardt GM,Dicks LM

更新日期：2006-04-01 00:00:00

abstract：AIMS:This study aimed to explore potential drug targets of Streptococcus suis at the system level. METHODS AND RESULTS:A homologous protein mapping method was used in the construction of a protein-protein interaction (PPI) network of S. suis, which presented 1147 nonredundant interaction pairs among 286 proteins. The ...

journal_title：Journal of applied microbiology

authors： Tan MF,Zou G,Wei Y,Liu WQ,Li HQ,Hu Q,Zhang LS,Zhou R

更新日期：2020-11-29 00:00:00

abstract：AIMS:The pathogenic potential of Arcobacter butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays. METHODS AND RESULTS:Five of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells...

journal_title：Journal of applied microbiology

authors： Karadas G,Bücker R,Sharbati S,Schulzke JD,Alter T,Gölz G

更新日期：2016-01-01 00:00:00

abstract：AIMS:To describe the occurrence and virulence gene pattern of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) in healthy goats of Jammu and Kashmir, India. METHODS AND RESULTS:A total of 220 E. coli strains belonging to 60 different 'O' serogroups was isolated from 206 local (nonmigra...

journal_title：Journal of applied microbiology

authors： Wani SA,Samanta I,Munshi ZH,Bhat MA,Nishikawa Y

更新日期：2006-01-01 00:00:00

abstract：AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...

journal_title：Journal of applied microbiology

authors： Crothers-Stomps C,Høj L,Bourne DG,Hall MR,Owens L

更新日期：2010-05-01 00:00:00

abstract：:Thirty-eight Lactococcus strains, isolated from raw milk produced in two dairy areas in Normandy, were identified at the phenotypic level. Only Lactococcus lactis strains with the lactis phenotype were found in the milk samples. Most strains fermented lactose (97%) and showed proteinase activity (76%). Isolates were c...

journal_title：Journal of applied microbiology

authors： Desmasures N,Mangin I,Corroler D,Guéguen M

更新日期：1998-12-01 00:00:00

abstract：:Differences in opacity between wells of a microtitre plate containing different volumes of inoculated growth medium reflected planktonic growth without any contribution from cells attached at the well surface. Simple algebra and a knowledge of the dependence of optical density upon sample path length (volume) for susp...

journal_title：Journal of applied microbiology

authors： Das JR,Bhakoo M,Jones MV,Gilbert P

更新日期：1998-05-01 00:00:00

abstract：AIMS:To evaluate beneficial effect of two food additives, ammonium molybdate (NH4-Mo) and sodium bicarbonate (NaBi), on antagonistic yeasts for control of brown rot caused by Monilinia fructicola in sweet cherry fruit under various storage conditions. The mechanisms of action by which food additives enhance the efficac...

journal_title：Journal of applied microbiology

authors： Qin GZ,Tian SP,Xu Y,Chan ZL,Li BQ

更新日期：2006-03-01 00:00:00

abstract：AIMS:Plants, fungal endophytes (FEs) and the changing environment interact with each other forming an interlaced network. This study evaluates nonadditive and interactive effects of the FE Acremonium strictum and drought treatment on Atractylodes lancea plantlets. METHODS AND RESULTS:By applying FEs (meristem cultures...

journal_title：Journal of applied microbiology

authors： Yang T,Ma S,Dai CC

更新日期：2014-11-01 00:00:00

abstract：AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) ,...

journal_title：Journal of applied microbiology

authors： Abdel-Hadi A,Carter D,Magan N

更新日期：2010-12-01 00:00:00

abstract：:The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...

journal_title：Journal of applied microbiology

authors： Méchin L,Dubois-Brissonnet F,Heyd B,Leveau JY

更新日期：1999-05-01 00:00:00

abstract：AIMS:In this study, pulsed-electric fields (PEF) and high-voltage electrical discharges (HVED) are proposed as new techniques for the microbial stabilization of red wines before bottling. The efficiency of the treatment was then evaluated. METHODS AND RESULTS:PEF and HVED-treatments have been applied to wine for the i...

journal_title：Journal of applied microbiology

authors： Delsart C,Grimi N,Boussetta N,Miot Sertier C,Ghidossi R,Vorobiev E,Mietton Peuchot M

更新日期：2016-01-01 00:00:00

abstract：AIMS:One of the major limitations to the production of alfalfa (Medicago sativa) is the fungus Phoma medicaginis, which infects alfalfa and causes leaf spots. This study aims to understand alfalfa's response to P. medicaginis infection, the colonization of arbuscular mycorrhizal fungus (AMF) and the effect of AMF on pl...

journal_title：Journal of applied microbiology

authors： Li Y,Duan T,Nan Z,Li Y

更新日期：2019-07-16 00:00:00

abstract：:Due to the increasing number of nosocomial infections and multidrug-resistant bacterial strains, Staphylococcus aureus is now a major worldwide concern. Rapid detection and characterization of this bacterium has become an important issue for biomedical applications. Biosensors are increasingly appearing as low-cost, e...

journal_title：Journal of applied microbiology

authors： Templier V,Roupioz Y

更新日期：2017-11-01 00:00:00

abstract：AIM:To determine the effect of different carbohydrate-based finishing diets on fermentation characteristics and the shedding of Escherichia coli and enterohaemorrhagic E. coli (EHEC) virulence genes in cattle faeces. METHODS AND RESULTS:The size of faecal E. coli populations and fermentation characteristics were ascer...

journal_title：Journal of applied microbiology

authors： Gilbert RA,Tomkins N,Padmanabha J,Gough JM,Krause DO,McSweeney CS

更新日期：2005-01-01 00:00:00

abstract：AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...

journal_title：Journal of applied microbiology

authors： Horakova K,Mlejnkova H,Mlejnek P

更新日期：2008-10-01 00:00:00

abstract：AIM:The aim of this study was to develop and evaluate bacterial-fungal communities to deconstruct switchgrass to biofuel precursors. METHODS AND RESULTS:Bacterial-fungal consortia, mesophilic (25°C) and thermophilic (50°C), were enriched from switchgrass bales from which enzyme mixtures were used to deconstruct delign...

journal_title：Journal of applied microbiology

authors： Jain A,Pelle HS,Baughman WH,Henson JM

更新日期：2017-04-01 00:00:00

abstract：AIMS:To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. METHODS AND RESULTS:After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated Bacillu...

journal_title：Journal of applied microbiology

authors： Wongputtisin P,Khanongnuch C,Khongbantad W,Niamsup P,Lumyong S

更新日期：2012-10-01 00:00:00

abstract：AIMS:To develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsrA) for use in quantitative real-time PCR detection of sulfate-reducing bacteria (SRB) in stored swine manure. METHODS AND RESULTS:Degenerate primer sets were developed to detect SRB in stored swine manure. These were comp...

journal_title：Journal of applied microbiology

authors： Spence C,Whitehead TR,Cotta MA

更新日期：2008-12-01 00:00:00

abstract：AIM:Aerobic CH4 oxidation is an important process controlling CH4 release from landfills to the atmosphere. The aim of this study was to investigate the link between CH4 oxidation activity and methanotrophs abundance and diversity in landfill cover soils of different age. METHODS AND RESULTS:Among the three investigat...

journal_title：Journal of applied microbiology

authors： Kong JY,Su Y,Zhang QQ,Bai Y,Xia FF,Fang CR,He R

更新日期：2013-09-01 00:00:00

abstract：AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...

journal_title：Journal of applied microbiology

authors： Lei V,Jakobsen M

更新日期：2004-01-01 00:00:00

abstract：AIM:Evaluation of the genetic variability of stolbur phytoplasma infecting grapevines, bindweeds and vegetables, collected in different central and southern Italian regions. MATERIALS AND RESULTS:Phytoplasma isolates belonging to stolbur subgroup 16SrXII-A were subjected to molecular characterization by polymerase cha...

journal_title：Journal of applied microbiology

authors： Murolo S,Marcone C,Prota V,Garau R,Foissac X,Romanazzi G

更新日期：2010-12-01 00:00:00

abstract：AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...

journal_title：Journal of applied microbiology

authors： Milesi MM,McSweeney PL,Hynes ER

更新日期：2008-09-01 00:00:00