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Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt.

Abstract：

AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metals such as nickel, cobalt and zinc and a group of antibiotics. Partial 16S rRNA analysis revealed that strain MA2 belonged to the genus Halomonas. Copper uptake, measured by atomic absorption spectrophotometery, was higher in the absence of NaCl than in the presence of 0.5-1.0 mol l(-1) NaCl during 5-15 min of incubation. Cell fractionation and electron microscopic observation clarified that most of the copper accumulated in the outer membrane and periplasmic fractions of the cells. Plasmid screening yielded two plasmids: pMA21 (11 kb) and pMA22 (5 kb). Plasmid curing resulted in a strain that lost both the plasmids and was sensitive to cobalt and chromate but not copper, nickel and zinc. This cured strain also showed weak growth in the presence of 0.5-1.0 mol l(-1) NaCl. Partial sequencing of both plasmids led to the identification of different toxic metals transporters but copper transporters were not identified. CONCLUSIONS:The highest cell viability was found in the presence of 1.0 mol l(-1) NaCl at different copper concentrations, and copper uptake was optimal in the absence of NaCl. Plasmid pMA21 encoded chromate, cobalt, zinc and cadmium transporters, whereas pMA22 encoded specific zinc and RND (resistance, nodulation, cell division) efflux transporters as well as different kinds of metabolic enzymes. Copper resistance was mainly incorporated in the chromosome. SIGNIFICANCE AND IMPACT OF THE STUDY:Strain MA2 is a fast and efficient tool for copper bioremediation and the isolated plasmids show significant characteristics of both toxic metal and antibiotic resistance.

journal_name

J Appl Microbiol

journal_title

Journal of applied microbiology

authors

Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

doi

10.1111/j.1365-2672.2009.04574.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1459-70

issue

eissn

1364-5072

issn

1365-2672

pii

JAM4574

journal_volume

108

pub_type

杂志文章

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Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt.