Abstract：
AIMS:To develop a protocol for environmental sampling to detect parvoviruses of dogs and cats in the environment. METHODS AND RESULTS:Environmental contamination was carried out using different dilutions of parvovirus-contaminated materials; further field samplings were performed in areas in which clinical cases of parvovirus infections were present. Sterile cotton swabs and sponges for microbial surface sampling were used. Viruses were detected in these samples with different methods: conventional PCR, nested PCR and real-time PCR, detecting viral DNA; virus isolation, detecting infectious virus; and a commercial rapid enzyme immunoassay, detecting viral antigen. No substantial differences were observed in the two sampling methods, although the sponge was more convenient for sampling rough surfaces. Molecular assays were the most sensitive methods, identifying even very low amounts of viral DNA (up to 10 copies of viral DNA/10 µl of sample). Virus isolation and the rapid test detected the viruses only at the highest viral concentrations, both in the experimental setting and field conditions. CONCLUSIONS:Environmental sampling and molecular protocols were effective in detecting environmental contamination with parvoviruses. SIGNIFICANCE AND IMPACT OF THE STUDY:The protocol will be useful to identify possible sources of infection and to assess the efficacy of disinfection protocols in the environment.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Marenzoni ML,Conti MB,Rossi E,Rinoldo R,Franceschini R,Tesei E,Porciello F,Mancini S,Favi B,Gamboni M,Boni P,Raffaele O,Desario C,Franciosini MP,Buonavoglia C,Decaro Ndoi
10.1111/jam.14971subject
Has Abstractpub_date
2020-12-16 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract：AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13516
更新日期：2017-09-01 00:00:00
abstract：AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05263.x
更新日期：2012-05-01 00:00:00
abstract：AIMS:To determine how hydrated Bacillus anthracis spores are killed in a high-temperature gas environment (HTGE), and how spores of several Bacillus species including B. anthracis are killed by UV radiation, dry heat, wet heat and desiccation. METHODS AND RESULTS:Hydrated B. anthracis spores were HTGE treated at c. 22...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12421
更新日期：2014-04-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01458.x
更新日期：2001-11-01 00:00:00
abstract：AIMS:Bacillus licheniformis PWD-1 is a keratin-degrading, spore-forming bacterium isolated from a poultry waste digester. A sporulation-deficient mutant of B. licheniformis PWD-1, named B. licheniformis WBG, was developed and characterized. METHODS AND RESULTS:The mutation was generated using the splicing by overlap e...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02515.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04755.x
更新日期：2010-10-01 00:00:00
abstract：AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13615
更新日期：2018-01-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03615.x
更新日期：2008-03-01 00:00:00
abstract：AIMS:To determine the effects of herbal essential oils on Trichophyton spp. growth and to evaluate the effects of Pelargonium graveolens oil and its main components citronellol and geraniol combined with ketoconazole against Trichophyton spp. METHODS AND RESULTS:Growth inhibition of six Trichophyton spp. by herbal ess...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02417.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To develop sensitive quantitative PCR assays for the two groups of pathogens responsible for Fusarium seedling blight in wheat: Fusarium group (Fusarium culmorum and Fusarium graminearum) and Microdochium group (Microdochium nivale and Microdochium majus); and to use the assays to assess performance of fungicide s...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03190.x
更新日期：2007-06-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13927
更新日期：2018-09-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02989.x
更新日期：2006-07-01 00:00:00
abstract：AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12960
更新日期：2015-12-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01974.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:To isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in Cuba without chemical fertilizers. METHODS AND RESULTS:Two N2-fixing isolates, 9C and T2, were obtained from surface-sterilized stems and roots, respectively, of sugarcane variety ML3-18. Both isolates showed acetylene reduction a...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02329.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatm...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04354.x
更新日期：2009-11-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14242
更新日期：2019-05-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00390.x
更新日期：1998-04-01 00:00:00
abstract：AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04597.x
更新日期：2010-06-01 00:00:00
abstract：:An extracellular phenolic acid esterase produced by the fungus Penicillium expansum in solid state culture released ferulic and rho-coumaric acid from methyl esters of the acids, and from the phenolic-carbohydrate esters O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->3)-O-beta- D-xylopyranosyl-(1-->4)-D-xylopy...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1997.00307.x
更新日期：1997-12-01 00:00:00
abstract：AIMS:To investigate the structural organization and dynamics of exopolysaccharides (EPS) matrix and microcolonies formation by Streptococcus mutans during the biofilm development process. METHODS AND RESULTS:Biofilms of Strep. mutans were formed on saliva-coated hydroxyapatite (sHA) discs in the presence of glucose or...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04616.x
更新日期：2010-06-01 00:00:00
abstract：AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12585
更新日期：2014-10-01 00:00:00
abstract：AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01297.x
更新日期：2001-05-01 00:00:00
abstract：AIMS:To clarify the effects of Lactobacillus gasseri CP2305 (CP2305) on quality of life and clinical symptoms and its functional mechanisms in patients with irritable bowel syndrome (IBS). METHODS AND RESULTS:After the patients were administered CP2305 daily for 4 weeks, the IBS-severity index score was significantly ...
journal_title：Journal of applied microbiology
pub_type： 临床试验,杂志文章
doi：10.1111/jam.13329
更新日期：2017-01-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12615
更新日期：2014-11-01 00:00:00
abstract：:An alginate gel bead artificial biofilm system was used to assay biofilm susceptibility to four biocides and to analyse the extent to which each agent penetrated the biofilm. Chlorine, glutaraldehyde, an isothiazolone, and a quaternary ammonium compound were tested on alginate-entrapped Enterobacter aerogenes in gel b...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.853529.x
更新日期：1998-09-01 00:00:00
abstract：AIMS:To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. METHODS AND RESULTS:One ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01874.x
更新日期：2003-01-01 00:00:00
abstract：:Since their discovery in 1915, bacteriophages have been routinely used within Eastern Europe to treat a variety of bacterial infections. Although initially ignored by the West due to the success of antibiotics, increasing levels and diversity of antibiotic resistance is driving a renaissance for bacteriophage-derived ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.12881
更新日期：2015-09-01 00:00:00
abstract：AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2005.02571.x
更新日期：2005-01-01 00:00:00
abstract：:Pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. Among the fungal diseases, blue mold caused by Penicillium expansum, grey mould caused by Botrytis cinerea, Mucor rot caused by Mucor piriformis are common on pear fruits. Other (weak) pathogens like Phi...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2672.2003.01920.x
更新日期：2003-01-01 00:00:00
