Abstract:
AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatments) were used to verify the effect of addition time of the variables on PV1 replication by crystal violet method and relative viral RNA quantification by real-time PCR for analysing in which step of virus replication the variables could interfere. CONCLUSIONS:Data revealed that the B. dracunculifolia showed the best antiviral activity percentage in the simultaneous treatment, as well as lower relative viral quantification by real-time PCR. Variables might block partially the viral entry within cells, affect the steps of viral cycle replication into cells, or lead to RNA degradation before the virus entry into cells or after their release to the supernatant. SIGNIFICANCE AND IMPACT OF THE STUDY:Baccharis dracunculifolia is the most important botanical source of the south-eastern Brazilian propolis, and its potential for the development of new phytotherapeutic medicines has been investigated. Propolis is commonly used for its antimicrobial and immunomodulatory activities. Nevertheless, B. dracunculifolia and propolis effects on PV1 have not been investigated yet.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Búfalo MC,Figueiredo AS,de Sousa JP,Candeias JM,Bastos JK,Sforcin JMdoi
10.1111/j.1365-2672.2009.04354.xsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
1669-80issue
5eissn
1364-5072issn
1365-2672pii
JAM4354journal_volume
107pub_type
杂志文章abstract::Shoe soles are possible vectors for infectious diseases. Although studies have been performed to assess the prevalence of infectious pathogens on shoe soles and decontamination techniques, no systematic review has ever occurred. The aim of this study was to perform a systematic review of the literature to determine th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13250
更新日期:2016-11-01 00:00:00
abstract::The goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13438
更新日期:2017-07-01 00:00:00
abstract:AIMS:The purpose of this study was to investigate the occurrence of Escherichia coli O157 and O26 on Belgian dairy cattle farms, the presence of virulence genes in the confirmed isolates and the association of E. coli O26 presence with calf diarrhoea. METHODS AND RESULTS:In total, 233 recto-anal mucosal swabs (RAMS) w...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14909
更新日期:2020-10-26 00:00:00
abstract::A Gram-positive rod was isolated from a commercial grape wine undergoing a sluggish/stuck alcoholic fermentation. The organism produced L-lactic acid from glucose, possessed weak catalase activity, and fermented relatively few carbohydrates, i.e. glucose, fructose, sucrose, raffinose (weakly) and mannitol. The 16S rRN...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00399.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo. METHODS AND RESULTS: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05241.x
更新日期:2012-04-01 00:00:00
abstract:AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04870.x
更新日期:2011-01-01 00:00:00
abstract::The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00821.x
更新日期:2000-01-01 00:00:00
abstract:AIM:Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. METHODS AND RESULTS:The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02163.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. METHODS AND RESULTS:Administration of th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13687
更新日期:2018-03-01 00:00:00
abstract:AIMS:To identify changes in the bacterial community, at the phylum level brought about by varied crop management. METHODS AND RESULTS:Next-generation sequencing methods were used to compare the taxonomic structure of the bacterial community within 24 agricultural soils managed with either organic or conventional metho...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12822
更新日期:2015-07-01 00:00:00
abstract:AIMS:To repurpose a silver-based antimicrobial textile coating product (Micro-Fresh 1911) as a dual-function antimicrobial laundry additive and textile coating. METHODS AND RESULTS:Survival of Escherichia coli or Staphylococcus aureus type and clinical isolates in a domestic 40°C wash was assessed with and without soi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14850
更新日期:2020-09-11 00:00:00
abstract:AIMS:To study the behaviour of salmonellae inoculated in the dry, raw materials, related to chocolate confectionery manufacture, as affected by the strain, cell preparation method and storage temperature. METHODS AND RESULTS:Outbreak and non outbreak-associated salmonellae were used for the inoculation of cocoa butter...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04144.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:Biofilms in water distribution systems represent a far more significant reservoir of micro-organisms than the water phase. Biofilms are (i) resistant to disinfectants, (ii) nuclei for microbial regrowth, (iii) a refuge for pathogens, (iv) accompanied by taste and odour problems, and (v) corrode surfaces. The effec...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01974.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01689.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. METHODS AND RESULTS:T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1). Suspension of T. scotoductus SA-01 cells also reduced Cr(VI) aero...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03429.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To investigate whether Photobacterium damselae subsp. piscicida (Phdp) can sense and directly respond to the presence of cationic antimicrobial peptides (AMPs). METHODS AND RESULTS:We performed proteomic methodologies to investigate the responsive proteins of Phdp on exposure to AMP Q6. Proteins significantly alt...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12672
更新日期:2015-01-01 00:00:00
abstract:AIMS:To determine the effects of herbal essential oils on Trichophyton spp. growth and to evaluate the effects of Pelargonium graveolens oil and its main components citronellol and geraniol combined with ketoconazole against Trichophyton spp. METHODS AND RESULTS:Growth inhibition of six Trichophyton spp. by herbal ess...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02417.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:This study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (MRCoPS) harboring in dogs and people associated with dogs in Thailand. METHODS AND RESULTS:Staphylococci were collected from 100 dogs, 100 dog owners, 200 small animal veterinarians and 100 people without pet...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12545
更新日期:2014-08-01 00:00:00
abstract:AIMS:To evaluate mannan oligosaccharide (MOS) and threonine effects on performance, small intestine morphology and Salmonella spp. counts in Salmonella Enteritidis-challenged birds. METHODS AND RESULTS:One-day-old chicks (1d) were distributed into five treatments: nonchallenged animals fed basal diet (RB-0), animals f...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12108
更新日期:2013-04-01 00:00:00
abstract::Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00257.x
更新日期:1997-08-01 00:00:00
abstract:AIMS:In vitro experiments were undertaken to evaluate biocide formulations commonly used in cooling water systems against protozoa previously isolated from cooling towers. The investigations evaluated the efficacy of these formulations against amoebic cysts and trophozoites. METHODS AND RESULTS:Laboratory challenges a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04044.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:To investigate the sort and the origin of the contamination of a packed fruit juice. METHODS AND RESULTS:Fifty-eight yeast isolates were collected in a survey of two different visits to a carbonated orange juice factory. In each visit, samples were collected, six times, from seven points in the production chain. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02542.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The purposes were to evaluate the detection of low levels of Campylobacter in water by dead-end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS:The DEUF technique followed by detection according to ISO 10272 was tested on artificially and natu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14379
更新日期:2019-10-01 00:00:00
abstract:AIMS:To examine associations between growth rate within bacterial populations and survival patterns following treatment with antimicrobial agents. METHODS AND RESULTS:Time survival data were generated for the inactivation of Escherichia coli populations, grown as batch and continuous cultures, exposed to ciprofloxacin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02079.x
更新日期:2003-01-01 00:00:00
abstract::The purpose of this article is to review coculture fermentations in industrial biotechnology. Examples for the advantageous utilization of cocultures instead of single cultivations include the production of bulk chemicals, enzymes, food additives, antimicrobial substances and microbial fuel cells. Coculture fermentati...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2009.04659.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14216
更新日期:2019-05-01 00:00:00
abstract::The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01021.x
更新日期:2000-04-01 00:00:00
abstract:AIMS:This project aimed to investigate the mechanism of attachment of the vegetative cells and spores of thermophilic bacilli to stainless steel with a view to devising strategies to limit biofilm development and survival. METHODS AND RESULTS:Spores and vegetative cells of bacterial isolates were exposed to protein de...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01323.x
更新日期:2001-06-01 00:00:00
abstract:AIMS:The aim of this study was to screen a chemical library consisting of over 1200 pharmacologically active, already approved off-patent compounds, to determine whether any of the compounds reduced or eliminated the invasion or intracellular replication phenotypes of Salmonella enterica serovar Typhimurium (S. Typhimu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13890
更新日期:2018-09-01 00:00:00