Abstract:
AIMS:To develop a predictive model for Escherichia coli using deep neural networks. METHODS AND RESULTS:Batch experiments are conducted at different temperatures closer to optimum value (36·5°C, 37°C, 37·5°C, 38°C and 38·5°C) to obtain the growth curves of E .coli K-12. Two primary models namely modified Gompertz and new logistic are chosen. Three secondary models namely Gaussian, nonlinear autoregressive eXogenous (NARX) model and long short-term memory (LSTM) are developed. The novelty in this paper is the development of secondary models using artificial neural network (ANN) and deep network. The performance measures chosen to compare the developed primary and secondary models are correlation coefficient (R2 ), root-mean-square error (RMSE) and accuracy factor (Af ). Results show that modified Gompertz model has better R2 (0·99) and RMSE (0·019) when compared to new logistic model. Also, the deep network model outperforms other secondary models. Based on the primary and novel secondary model, a predictive model (tertiary model) is developed with improved accuracy and is validated. CONCLUSIONS:The proposed predictive model exhibit good validation results in terms of RMSE and R2 values and can be applied for determining the growth rate of E. coli at a particular temperature value. SIGNIFICANCE AND IMPACT OF THE STUDY:The proposed model can be used in food processing industries during enzyme production such as Chymosin, to predict the growth rate of E. coli as a function of temperature. Also, the developed LSTM and NARX models can be used to predict maximum specific growth rate of other microbial strains with proper training.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Chitra M,Sutha S,Pappa Ndoi
10.1111/jam.14901subject
Has Abstractpub_date
2020-10-16 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract::We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemilumine...
journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.1997.00368.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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pub_type: 杂志文章
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2003.01803.x
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00829.x
更新日期:1999-12-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2008.04008.x
更新日期:2009-02-01 00:00:00
abstract::Intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. The Gram-positive human pathogen Listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. Besides three penicilli...
journal_title:Journal of applied microbiology
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abstract:AIMS:The ComPath project is a pan-European programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (MIC) determination. Here, the susceptibility of major pathogens is reported from antimicr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
abstract:AIMS:The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. METHODS AND RESULTS:Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66...
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abstract:AIMS:Root rot caused by a group of fungi is a serious disease in mulberry. This study aims to identify and characterize Rhizopus oryzae and other fungal species associated with root rot of mulberry in India. METHODS AND RESULTS:Rotted root samples were collected from the mulberry gardens from four states of Southern I...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1111/jam.12673
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.00821.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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abstract:AIMS:To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo. METHODS AND RESULTS: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w)...
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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abstract:AIMS:The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). METHODS AND RESULTS:Forty-three per...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03551.x
更新日期:2008-01-01 00:00:00
abstract:AIMS:To analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. METHODS AND RESULTS:By using random amplified polymorphic DNA (RAPD) P...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.12858
更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04152.x
更新日期:2009-06-01 00:00:00