Bifidobacteria as indicators of faecal contamination along a sheep meat production chain.

Abstract:

AIMS:The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). METHODS AND RESULTS:Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. CONCLUSIONS:Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. SIGNIFICANCE AND IMPACT OF THE STUDY:Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.

journal_name

J Appl Microbiol

authors

Delcenserie V,Loncaric D,Bonaparte C,Upmann M,China B,Daube G,Gavini F

doi

10.1111/j.1365-2672.2007.03551.x

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

276-84

issue

1

eissn

1364-5072

issn

1365-2672

pii

JAM3551

journal_volume

104

pub_type

杂志文章