Abstract:
AIMS:The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). METHODS AND RESULTS:Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. CONCLUSIONS:Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. SIGNIFICANCE AND IMPACT OF THE STUDY:Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Delcenserie V,Loncaric D,Bonaparte C,Upmann M,China B,Daube G,Gavini Fdoi
10.1111/j.1365-2672.2007.03551.xsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
276-84issue
1eissn
1364-5072issn
1365-2672pii
JAM3551journal_volume
104pub_type
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更新日期:2015-07-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.01109.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2008-01-01 00:00:00
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2020-07-20 00:00:00
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更新日期:2018-08-01 00:00:00