Abstract:
AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the wild-type strain; two mutants exhibited enhanced biofilm formation and one produced less biofilm biomass than the wild-type. The mutant 15G01 mprF::Himar1, which had a transposon insertion in the mprF gene, was selected for further analysis. The mutant produced a more densely populated biofilm on solid surfaces such as stainless steel and polystyrene, as determined using scanning electron and light microscopy. The 15G01 mprF::Himar1 mutant remained viable in biofilms, but showed an increase in sensitivity to the cationic antimicrobial gallidermin. The mutant also displayed reduced invasiveness in CaCo-2 intestinal cells, suggesting virulence properties are compromised by the inactivation of mprF. CONCLUSIONS:Biofilm formation and gallidermin resistance of L. monocytogenes is influenced by mprF, but this trait is associated with a compromise in invasiveness. SIGNIFICANCE AND IMPACT OF THE STUDY:The presence of pathogenic microorganisms in the food processing environment can cause a significant problem, especially when these microorganisms are established as biofilms. This study shows that the inactivation of the mprF gene results in enhanced biofilm formation and abiotic surface attachment of L. monocytogenes.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Nowak J,Visnovsky SB,Cruz CD,Fletcher GC,van Vliet AHM,Hedderley D,Butler R,Flint S,Palmer J,Pitman ARdoi
10.1111/jam.14790subject
Has Abstractpub_date
2020-07-20 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract:AIMS:To isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (SPs) in sheep dips. METHODS AND RESULTS:SP-degrading bacteria were isolated from a mixed soil sample consisting of garden soil and soils from farms where SPs had been used. The two largest in size were then iden...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01558.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05296.x
更新日期:2012-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02329.x
更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13085
更新日期:2016-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12980
更新日期:2016-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04561.x
更新日期:2010-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01227.x
更新日期:2001-01-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12723
更新日期:2015-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00431.x
更新日期:1998-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02244.x
更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04643.x
更新日期:2010-07-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03117.x
更新日期:2007-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03511.x
更新日期:2007-12-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04008.x
更新日期:2009-02-01 00:00:00
abstract:AIMS:The transfer of tetO gene conferring resistance to tetracycline was studied between Campylobacter jejuni strains, in the digestive tract of chickens. METHODS AND RESULTS:In vitro conjugation experiments were first performed in order to select donor/recipient couples for further in vivo assay. Then, chickens were ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02306.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The aims of this study were to: (1) estimate the effectiveness of ultraviolet radiation (UV) and sulfuric-acid based fertilizer (SA), at reducing levels of generic Escherichia coli in surface irrigation water and on produce and surface soil in open produce fields; and (2) describe the population dynamics of generi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.15011
更新日期:2021-01-22 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04192.x
更新日期:2009-07-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04100.x
更新日期:2009-05-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00938.x
更新日期:1999-12-01 00:00:00
abstract::This review is an account of experiences of two research teams (from Italy to Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. This review covers th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13548
更新日期:2017-12-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14092
更新日期:2018-08-28 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04301.x
更新日期:2009-10-01 00:00:00
abstract:AIMS:To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. METHODS AND RESULTS:Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02942.x
更新日期:2006-08-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01089.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:To develop and evaluate cross-priming amplification (CPA) combined with immuno-blotting for the detection of coagulase-positive Staphylococci including Staphylococcus aureus. METHODS AND RESULTS:Twenty-four sets of cross and detection primers were designed according to four sequences of coagulase gene in Staph. a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12836
更新日期:2015-07-01 00:00:00
abstract:AIM:Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts was studied with different compost types, storage conditions and inoculum levels. METHODS AND RESULTS:A cocktail of three E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13719
更新日期:2018-05-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02242.x
更新日期:2004-01-01 00:00:00
abstract:AIM:To develop a probiotic with effectiveness against Aeromonas sp., which was pathogenic to rainbow trout. METHODS AND RESULTS:When Bacillus subtilis AB1, which was obtained from fish intestine, was administered for 14 days to rainbow trout in feed at a concentration of 10(7) cells per gram either as viable, formaliz...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03402.x
更新日期:2007-11-01 00:00:00