Development of a novel triplex PCR assay for the detection and differentiation of thermophilic species of Campylobacter using 16S-23S rDNA internal transcribed spacer (ITS) region.

abstract：AIMS:Vibrio vulnificus is a common bacterial pathogen causing haemorrhagic septicaemia in eel farming. This study investigates the immunogenicity of an outer membrane protein U (OmpU) of V. vulnificus and the feasibility of the protein as a new subunit vaccine against V. vulnificus. METHODS AND RESULTS:Partial gene se...

journal_title：Journal of applied microbiology

authors： Le H,LiHua D,JianJun F,Peng L,SongLin G

更新日期：2018-08-14 00:00:00

abstract：AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

journal_title：Journal of applied microbiology

authors： Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

更新日期：2011-01-01 00:00:00

abstract：AIMS:To determine whether strains of Geobacillus stearothermophilus isolated from a milk powder manufacturing plant were different in their ability to form biofilms and produce spores. In addition, this study evaluated whether there were other physiological characteristics that could differentiate these strains. METHO...

journal_title：Journal of applied microbiology

authors： Burgess SA,Flint SH,Lindsay D

更新日期：2014-02-01 00:00:00

abstract：AIMS:This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes. METHODS AND RESULTS:Water and copper pipes were collected from a cold-...

journal_title：Journal of applied microbiology

authors： Pavissich JP,Vargas IT,González B,Pastén PA,Pizarro GE

更新日期：2010-09-01 00:00:00

abstract：AIMS:Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). METHODS AND RESULTS:Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. B...

journal_title：Journal of applied microbiology

authors： Khalil MA,Azzazy HM,Attia AS,Hashem AG

更新日期：2014-08-01 00:00:00

abstract：AIMS:To avoid interference by water-iodine disinfection chemistry and measure directly the effect of iodine, captured from a triiodide complex bound to a filter medium, on viability of penetrating viral particles. METHODS AND RESULTS:Aerosols of MS2 coli phage were passed through control P100 or iodinated High-Efficie...

journal_title：Journal of applied microbiology

authors： Heimbuch BK,Harnish DA,Balzli C,Lumley A,Kinney K,Wander JD

更新日期：2015-06-01 00:00:00

abstract：AIMS:To investigate the potentials and limitations of Fourier transform-infrared (FT-IR) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment. METHODS AND RESULTS:A total of 1570 FT-IR spectra from 164 gram-positive and gram-negativ...

journal_title：Journal of applied microbiology

authors： Sandt C,Madoulet C,Kohler A,Allouch P,De Champs C,Manfait M,Sockalingum GD

更新日期：2006-10-01 00:00:00

abstract：AIMS:To investigate the effect of various single nutrient deficiencies on poly-beta-hydroxybutyrate (PHB) biosynthesis in a methane-utilizing mixed culture (dominant species Methylocystis sp. GB 25 DSM 7674). METHODS AND RESULTS:Poly-beta-hydroxybutyrate accumulation experiments were performed in 7 and 70 l bioreactor...

journal_title：Journal of applied microbiology

authors： Helm J,Wendlandt KD,Jechorek M,Stottmeister U

更新日期：2008-10-01 00:00:00

abstract：:Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

journal_title：Journal of applied microbiology

authors： George I,Petit M,Servais P

更新日期：2000-03-01 00:00:00

abstract：AIMS:To evaluate the protective efficacy of Pediococcus pentosaceus GS4 in cadmium (Cd)-induced toxicity in Swiss albino mice. METHODS AND RESULTS:The previously reported probiotic P. pentosaceus GS4 interaction with Cd was evaluated experimentally. Swiss albino mice were segregated randomly into three different group...

journal_title：Journal of applied microbiology

authors： Dubey V,Mishra AK,Ghosh AR,Mandal BK

更新日期：2019-04-01 00:00:00

abstract：AIM:The aim of this study was to characterize the microbial community involved in anaerobic degradation of petroleum hydrocarbon under low- and moderate-temperature conditions. METHODS AND RESULTS:Sulfate-reducing enrichment cultures growing on crude oil and p-xylene were established at low and moderate temperatures. ...

journal_title：Journal of applied microbiology

authors： Higashioka Y,Kojima H,Fukui M

更新日期：2011-01-01 00:00:00

abstract：AIMS:To determine the incidence of emetic toxin producing Bacillus cereus in soil, animal faeces and selected vegetable produce to compare the results with the previously reported high incidence in rice paddy fields. To examine whether the emetic toxin has antibiotic activity. METHODS AND RESULTS:The incidence of emet...

journal_title：Journal of applied microbiology

authors： Altayar M,Sutherland AD

更新日期：2006-01-01 00:00:00

abstract：AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...

journal_title：Journal of applied microbiology

authors： Luepongpattana S,Thaniyavarn J,Morikawa M

更新日期：2017-12-01 00:00:00

abstract：:Heterotrophic plate count (HPC) bacteria are naturally present in all aqueous environments. These bacteria undergo multiplication cycles in drinking water, especially in closed containers (bottled water) or in tap water when chlorine levels are dissipated, such as in dead ends in water mains or household plumbing. A s...

journal_title：Journal of applied microbiology

authors： Edberg SC,Kops S,Kontnick C,Escarzaga M

更新日期：1997-04-01 00:00:00

abstract：AIMS:To better understand antibiotic resistance of Enterobacter cloacae isolates originated from food animals, the phenotypic and genotypic resistance of Ent. cloacae isolates from retail ground beef, cattle farm, processing facilities and clinical settings were investigated. METHODS AND RESULTS:The ampC, ampD and amp...

journal_title：Journal of applied microbiology

authors： Kim SH,Wei CI

更新日期：2007-08-01 00:00:00

abstract：AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...

journal_title：Journal of applied microbiology

authors： Pritchard MF,Jack AA,Powell LC,Sadh H,Rye PD,Hill KE,Thomas DW

更新日期：2017-09-01 00:00:00

abstract：AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...

journal_title：Journal of applied microbiology

authors： Perry JD,James AL,Morris KA,Oliver M,Chilvers KF,Reed RH,Gould FK

更新日期：2006-11-01 00:00:00

abstract：AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...

journal_title：Journal of applied microbiology

authors： Asvapathanagul P,Olson BH

更新日期：2017-01-01 00:00:00

abstract：AIMS:To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects. METHODS AND RESULTS:Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using g...

journal_title：Journal of applied microbiology

authors： Castanheira N,Dourado AC,Kruz S,Alves PI,Delgado-Rodríguez AI,Pais I,Semedo J,Scotti-Campos P,Sánchez C,Borges N,Carvalho G,Barreto Crespo MT,Fareleira P

更新日期：2016-03-01 00:00:00

abstract：AIMS:Ascospores of Talaromyces macrosporus are constitutively dormant and germinate after a strong external shock, classically a heat treatment. This fungus is used as a model system to study heat resistance leading to food spoilage after pasteurization. This study evaluates the effect of high pressure on the germinati...

journal_title：Journal of applied microbiology

authors： Dijksterhuis J,Teunissen PG

更新日期：2004-01-01 00:00:00

abstract：AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...

journal_title：Journal of applied microbiology

authors： Li X,Nie J,Hammill DL,Smith D,Xu H,De Boer SH

更新日期：2014-10-01 00:00:00

abstract：AIMS:  To determine the capacity of secondary metabolite of strain SX-4, to enhance the nonspecific immunity and survival of carp (Cyprinus carpio), and to identify the constituents that are responsible. METHODS AND RESULTS:  A thermophilic strain SX-4 that is able to produce immunostimulatory metabolite was isolated ...

journal_title：Journal of applied microbiology

authors： Liu J,Lei Y,Wang F,Yi Y,Liu Y,Wang G

更新日期：2011-04-01 00:00:00

abstract：AIMS:To understand the mode of action of thyme essential oil as an alternative biofungicide. METHODS AND RESULTS:The chemical composition of thyme essential oil isolated by hydrodistillation from the aerial parts of Thymus vulgaris was analyzed. The main constituents of thyme essential oil were thymol (76·96%), ρ-cyme...

journal_title：Journal of applied microbiology

authors： Ben Jabeur M,Somai-Jemmali L,Hamada W

更新日期：2017-04-01 00:00:00

abstract：AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...

journal_title：Journal of applied microbiology

authors： Kairyte K,Lapinskas S,Gudelis V,Luksiene Z

更新日期：2012-06-01 00:00:00

abstract：AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...

journal_title：Journal of applied microbiology

authors： Mimee B,Pelletier R,Bélanger RR

更新日期：2009-09-01 00:00:00

abstract：AIMS:Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in ...

journal_title：Journal of applied microbiology

authors： Coronel-León J,Marqués AM,Bastida J,Manresa A

更新日期：2016-01-01 00:00:00

abstract：AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...

journal_title：Journal of applied microbiology

authors： Cambon-Bonavita MA,Raguénès G,Jean J,Vincent P,Guezennec J

更新日期：2002-01-01 00:00:00

abstract：:The rumen contents of red deer (Cervus elaphus) were used to isolate bacterial capable of fermenting glycerol. The biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. The isolate (DR3) was tentatively identified as a strain of the species Klebsiella planticola as based on phenotyp...

journal_title：Journal of applied microbiology

authors： Jarvis GN,Moore ER,Thiele JH

更新日期：1997-08-01 00:00:00

abstract：AIMS:To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. METHODS AND RESULTS:The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied using a microtitre plate...

journal_title：Journal of applied microbiology

authors： Elgalai I,Foster HA

更新日期：2003-01-01 00:00:00

abstract：:Considerable investment has been made in recent years in improvements to the microbiological quality of urban wastewater discharges to surface waters, particularly in coastal towns, with the aim of reducing the exposure of bathers and surfers to gastrointestinal pathogens. As this source of pollution has come under gr...

journal_title：Journal of applied microbiology

authors： Tyrrel SF,Quinton JN

更新日期：2003-01-01 00:00:00