Abstract:
:Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically needed to monitor the emergence, transmission, and colonization of this pathogen in the animal reservoir. In this study, a novel multiplex polymerase chain reaction (PCR) assay was developed by using 5 sets of primers that specifically amplify segments of the eaeA, slt-I, slt-II, fliC, rfbE genes, which allowed simultaneous identification of serotype O157:H7 and its virulence factors in a single reaction. Analysis of 82 E. coli strains (49 O157:H7 and 33 non-O157:H7) demonstrated that this PCR system successfully distinguished serotype O157:H7 from other serotypes of E. coli and provided accurate profiling of the shiga-like toxins and the intimin adhesin in individual strains. This multiplex PCR assay did not cross-react with the background bacterial flora in bovine faeces and could detect a single O157:H7 organism per gram of faeces when combined with an enrichment step. Together, these results indicate that the multiplex PCR assay can be used for specific identification and profiling of E. coli O157:H7 isolates, and may be applied to rapid and sensitive detection of E. coli O157:H7 in bovine faeces when combined with an enrichment step.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Hu Y,Zhang Q,Meitzler JCdoi
10.1046/j.1365-2672.1999.00938.xsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
867-76issue
6eissn
1364-5072issn
1365-2672journal_volume
87pub_type
杂志文章abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03042.x
更新日期:2006-12-01 00:00:00
abstract:AIMS:To study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. METHODS AND RESULTS:Seed of rice cultivar PSBRc14 was collected from farmers' fields of irrigated lowland in southern Luzon, Philippines. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04268.x
更新日期:2009-09-01 00:00:00
abstract:AIMS:The aims of this study were to determine the occurrence of Fusarium graminearum species complex (FGSC) on soybean pods, seeds and roots, including rhizoplane, during the period of soybean crop in rotation with wheat and to evaluate the FGSC dynamics on wheat and soybean residues during two soybean growing seasons ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14765
更新日期:2021-01-01 00:00:00
abstract:AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02058.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:In vitro experiments were undertaken to evaluate biocide formulations commonly used in cooling water systems against protozoa previously isolated from cooling towers. The investigations evaluated the efficacy of these formulations against amoebic cysts and trophozoites. METHODS AND RESULTS:Laboratory challenges a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04044.x
更新日期:2009-03-01 00:00:00
abstract::Pathogens express virulence genes in response to the combination of environmental conditions present in the host environment. The crop is the first gastrointestinal environment encountered in birds. However, feed withdrawal alters the crop environment resulting in an increased pH, and decreased concentrations of lacta...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01089.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:The formation of metabolically inactive and nongrowing cells is an inevitable by-product of intensive fermentation. This study investigated whether co-feeding can be used to resuscitate nongrowing Acetobacter senegalensis cells to enable them to produce gluconic acid in successive fermentation runs at 38°C. METHO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14371
更新日期:2019-10-01 00:00:00
abstract:AIMS:To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS:The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13290
更新日期:2016-12-01 00:00:00
abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02159.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:(i) To analyse the increase in calcium ion uptake caused by several cationic dyes on Candida albicans, (ii) to postulate a mechanism, (iii) to define the effects of Zn ions on the phenomenon, and (iv) to propose the use of the dyes or their derivatives against C. albicans. METHODS AND RESULTS:Cells were grown in ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14092
更新日期:2018-08-28 00:00:00
abstract:AIM:This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. METHODS AND RESULTS:Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03313.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:Lactococcus lactis N8 and Saccharomyces boulardii SAA655 were investigated for their ability to synthesize B-vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. METHODS AND RESULTS:In this study, ultra-high performance liquid chromatography and microbiological as...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13453
更新日期:2017-06-01 00:00:00
abstract:AIMS:This study was applied to evaluate the usefulness of a high-throughput sample preparation protocol prior to the application of quantitative real-time PCR (qPCR) for the early diagnosis of bloodstream and pyogenic infections in humans and animals compared to matrix-assisted laser desorption ionization-time of fligh...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14862
更新日期:2020-10-18 00:00:00
abstract:AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01767.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Due to their antimicrobial activity, silver nanoparticles (Ag-NPs) are being increasingly used in a number of industrial products. The accumulation of Ag-NPs in the soil might affect plant growth-promoting rhizobacteria and, in turn, the plants. We describe the effects of Ag-NPs on the soil bacteria Azotobacter vi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12779
更新日期:2015-05-01 00:00:00
abstract:AIMS:Monitoring for Salmonella in slaughter pigs is important to enable targeted control measures to be applied on problem farms and at the abattoir. The aim of this study was to determine whether pooled serum and meat juice could be used to identify finishing pig herds with a high prevalence of infection. METHODS AND...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1365-2672.2003.02070.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. METHODS AND RESULTS:One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Anti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14999
更新日期:2021-01-09 00:00:00
abstract::Intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. The Gram-positive human pathogen Listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. Besides three penicilli...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.12989
更新日期:2016-02-01 00:00:00
abstract:AIMS:To see the possibility of particle size distribution analyser (PSDA) in detecting concentration of lactobacillus contaminants in yeast fermentation. METHODS AND RESULTS:A PSDA was used to rapidly determine the size and concentration of lactobacillus and Saccharomyces cerevisiae. Data showed that the aerodynamic d...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04331.x
更新日期:2009-11-01 00:00:00
abstract::The effect of divalent cations on the inactivation of Escherichia coli by high hydrostatic pressure was investigated. The presence of 0.5 mmol l-1 of CaCl2, MgCl2, MnCl2 and FeCl2 reduced pressure inactivation of E. coli MG1655, while 0.5 mmol l-1 of ZnCl2, NiCl2, CuCl2 and CoCl2 increased inactivation. Baroprotection...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1998.00577.x
更新日期:1998-10-01 00:00:00
abstract::This review is an account of experiences of two research teams (from Italy to Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. This review covers th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13548
更新日期:2017-12-01 00:00:00
abstract:AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04192.x
更新日期:2009-07-01 00:00:00
abstract::Nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (CLA) from free linoleic acid in vitro. Two strains of Propionibacterium freudenreichii ssp. freudenreichii and one strain ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00481.x
更新日期:1998-07-01 00:00:00
abstract:AIMS:The objective of this study was to determine the technological behaviour (implantation and biogenic amines production) of Carnobacterium divergens V41, an anti-Listeria bacteriocin producer (divercin V41), after inoculation in cold smoked salmon (CSS). METHODS AND RESULTS:Implantation of the strain was followed b...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01561.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. METHODS:Putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03338.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:Bacteriophages infect bacteria, and they are present everywhere in the world including the intestinal tracts of animals. Yersiniosis is a common foodborne infection caused by Yersinia enterocolitica and Yersinia pseudotuberculosis. As these bacteria are frequently isolated from pigs, we wanted to know whether Yers...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12722
更新日期:2015-03-01 00:00:00
abstract:AIMS:To analyse and compare the effect of selection power for antimicrobial resistance (AMR) in coliforms of two kinds of β-lactams-aminopenicillins; ampicillin (Amp) and cephalosporins; cephalexin (Cpn) and ceftiofur (Cef)-and tetracycline (Tet) using an approach based on a swine faecal microcosmos. METHODS AND RESUL...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14721
更新日期:2020-11-01 00:00:00
abstract:AIMS:Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14190
更新日期:2019-04-01 00:00:00
abstract:AIMS:To develop a suite of group-specific, rRNA-targeted oligonucleotide scissor probes for the quantitative detection of the predominant bacterial groups within the ruminal microbial community with the rRNA cleavage reaction-mediated microbial quantification method. METHODS AND RESULTS:Oligonucleotides that complemen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03443.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12585
更新日期:2014-10-01 00:00:00