Abstract:
AIMS:To see the possibility of particle size distribution analyser (PSDA) in detecting concentration of lactobacillus contaminants in yeast fermentation. METHODS AND RESULTS:A PSDA was used to rapidly determine the size and concentration of lactobacillus and Saccharomyces cerevisiae. Data showed that the aerodynamic diameters of Lactobacillus casei and S. cerevisiae cells were around 0.63 and 2.9 microm, respectively, with both cultures showing a linear relationship between cell density and particle count on a size distribution curve of PSDA. In addition, Lactobacillus fermentum showed high similarity in bacterial size distribution and particle count numbers with L. casei. The PSDA also rapidly detected (within 1 min) the cell concentrations of S. cerevisiae and L. casei in a mixed sample with different concentration ratios with 10(7)-10(9) cells ml(-1) of detection range. CONCLUSIONS:PSDA was demonstrated to be useful for the rapid detection of lactobacillus and S. cerevisiae concentrations. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first report concerning PSDA to detect the concentration of bacteria and yeast. This method can be useful in the actual field during ethanol fermentation because of relatively easy handling and rapid detection.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Kim H,Park K,Oh S,Chang ISdoi
10.1111/j.1365-2672.2009.04331.xsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
1499-504issue
5eissn
1364-5072issn
1365-2672pii
JAM4331journal_volume
107pub_type
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