Multiple competitive PCR-DGGE as a tool for quantifying and profiling defined mixed cultures of lactic acid bacteria during production of probiotics from complex polysaccharides.

abstract：:The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...

journal_title：Journal of applied microbiology

authors： Ribas F,Perramon J,Terradillos A,Frias J,Lucena F

更新日期：2000-04-01 00:00:00

abstract：AIMS:The purposes were to evaluate the detection of low levels of Campylobacter in water by dead-end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS:The DEUF technique followed by detection according to ISO 10272 was tested on artificially and natu...

journal_title：Journal of applied microbiology

authors： Ferrari S,Frosth S,Svensson L,Fernström LL,Skarin H,Hansson I

更新日期：2019-10-01 00:00:00

abstract：AIMS:The trans-10,cis-12 conjugated linoleic acid (CLA) is known for its antilipogenic effect but the mechanism is not fully clear. In this study, the potential of yeast (Saccharomyces cerevisiae) metabolism to offer evidence for the mechanism was investigated. METHODS AND RESULTS:The inhibitory effect of CLA on lipid...

journal_title：Journal of applied microbiology

authors： Hokkanen S,Laakso S,Senn CM,Frey AD

更新日期：2017-07-01 00:00:00

abstract：AIM:To evaluate the effect of NaCl, growth medium and temperature on the antimicrobial activity of bovine lactoferrin (LF) against Escherichia coli O157:H7 in the presence of different chelating agents. METHODS AND RESULTS:LF (32 mg ml(-1)) was tested against E. coli O157:H7 strain 3081 in Luria broth (LB) and All Pur...

journal_title：Journal of applied microbiology

authors： Al-Nabulsi AA,Holley RA

更新日期：2006-02-01 00:00:00

abstract：:New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E-78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram-negative test organism, Pantoea agglomerans (Enterobacter...

journal_title：Journal of applied microbiology

authors： Niku-Paavola ML,Laitila A,Mattila-Sandholm T,Haikara A

更新日期：1999-01-01 00:00:00

abstract：AIM:Introduction of a protocol for broad-range diagnosis of bacterial infections, which remain negative in culture. METHODS AND RESULTS:The new TaqMan real-time PCR assay amplifies part of the 16S rRNA gene. Species are identified by subsequent sequencing and phylogenetic blast analysis. The analytical sensitivity sho...

journal_title：Journal of applied microbiology

authors： Schabereiter-Gurtner C,Nehr M,Apfalter P,Makristathis A,Rotter ML,Hirschl AM

更新日期：2008-04-01 00:00:00

abstract：AIMS:To evaluate the sensitivity and specificity of the BioFire Diagnostics FilmArray(®) system in combination with their Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft) and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. METHODS AND RESULTS:DNA samples f...

journal_title：Journal of applied microbiology

authors： Seiner DR,Colburn HA,Baird C,Bartholomew RA,Straub T,Victry K,Hutchison JR,Valentine N,Bruckner-Lea CJ

更新日期：2013-04-01 00:00:00

abstract：AIMS:Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products. METHODS AND RESULTS:Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utili...

journal_title：Journal of applied microbiology

authors： Wagner RD,Paine DD,Cerniglia CE

更新日期：2003-01-01 00:00:00

abstract：:Medical devices provide critical care and diagnostic applications through patient contact. Sterility assurance level (SAL) may be defined as the probability of a single viable micro-organism occurring on an item after a sterilization process. Sterilization microbiology often relies upon using an overkill validation me...

journal_title：Journal of applied microbiology

authors： McEvoy B,Lynch M,Rowan NJ

更新日期：2020-11-06 00:00:00

abstract：AIMS:Coagulase-negative Staphylococcus xylosus strains are used as starter organisms for sausage fermentation. As those strains have to cope with low pH-values during fermentation, the aim of this study was to identify the acid adaptation mechanisms of S. xylosus TMW 2.1523 previously isolated from salami. METHODS AND...

journal_title：Journal of applied microbiology

authors： Kolbeck S,Behr J,Vogel RF,Ludwig C,Ehrmann MA

更新日期：2019-05-01 00:00:00

abstract：AIMS:The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng). ...

journal_title：Journal of applied microbiology

authors： Sharma D,Mal G,Kannan A,Bhar R,Sharma R,Singh B

更新日期：2017-11-01 00:00:00

abstract：AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...

journal_title：Journal of applied microbiology

authors： Hellström A,Qvirist L,Svanberg U,Veide Vilg J,Andlid T

更新日期：2015-05-01 00:00:00

abstract：AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of t...

journal_title：Journal of applied microbiology

authors： Lee TH,Kim MD,Park YC,Bae SM,Ryu YW,Seo JH

更新日期：2003-01-01 00:00:00

abstract：AIMS:To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. METHODS AND RESULTS:A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic propertie...

journal_title：Journal of applied microbiology

authors： Miñana-Galbis D,Farfán M,Lorén JG,Fusté MC

更新日期：2002-01-01 00:00:00

abstract：AIMS:Isomalto-oligosaccharides (IMO) with α(1 --> 6) and α(1 --> 4) glucosidic linkages are produced by enzymatic conversion of starch. IMO are only partially digestible but data on their influence on intestinal microbiota are limited. It was the aim of this study to investigate the effect of IMO diet on intestinal mic...

journal_title：Journal of applied microbiology

authors： Ketabi A,Dieleman LA,Gänzle MG

更新日期：2011-05-01 00:00:00

abstract：AIMS:To repurpose a silver-based antimicrobial textile coating product (Micro-Fresh 1911) as a dual-function antimicrobial laundry additive and textile coating. METHODS AND RESULTS:Survival of Escherichia coli or Staphylococcus aureus type and clinical isolates in a domestic 40°C wash was assessed with and without soi...

journal_title：Journal of applied microbiology

authors： Owen L,Laird K

更新日期：2020-09-11 00:00:00

abstract：AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...

journal_title：Journal of applied microbiology

authors： Young SB,Setlow P

更新日期：2004-01-01 00:00:00

abstract：AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The effica...

journal_title：Journal of applied microbiology

authors： Wang J,Qiu J,Dong J,Li H,Luo M,Dai X,Zhang Y,Leng B,Niu X,Zhao S,Deng X

更新日期：2011-12-01 00:00:00

abstract：:The enzymatic activity and viability of Escherichia coli O157:H7 in natural river water was determined by flow cytometry. River water was collected at two sites (an agricultural area and an industrial area) on the Aigawa River (Osaka, Japan). To facilitate estimation of the physiology of E. coli O157 in natural river ...

journal_title：Journal of applied microbiology

authors： Tanaka Y,Yamaguchi N,Nasu M

更新日期：2000-02-01 00:00:00

abstract：AIMS:Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also a...

journal_title：Journal of applied microbiology

authors： Nikel PI,Ramirez MC,Pettinari MJ,Méndez BS,Galvagno MA

更新日期：2010-08-01 00:00:00

abstract：:Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the...

journal_title：Journal of applied microbiology

authors： Bennett AR,Davids FG,Vlahodimou S,Banks JG,Betts RP

更新日期：1997-08-01 00:00:00

abstract：AIMS:In vitro experiments were undertaken to evaluate biocide formulations commonly used in cooling water systems against protozoa previously isolated from cooling towers. The investigations evaluated the efficacy of these formulations against amoebic cysts and trophozoites. METHODS AND RESULTS:Laboratory challenges a...

journal_title：Journal of applied microbiology

authors： Critchley M,Bentham R

更新日期：2009-03-01 00:00:00

abstract：:Recent advances in DNA sequencing technology have made it possible to elucidate the entire genomes of pathogenic bacteria, and advancements in bioinformatic tools have driven comparative studies of these genome sequences. These evaluations are dramatically increasing our ability to make valid considerations of the lim...

journal_title：Journal of applied microbiology

authors： McDevitt D,Payne DJ,Holmes DJ,Rosenberg M

更新日期：2002-01-01 00:00:00

abstract：:Ecological and physiological effects of the sulphate-reducing bacterium (SRB) Desulfovibrio desulfuricans on other intestinal organisms were investigated in anaerobic chemostats (dilution rate approximately 0.2 h-1). Reproducible defined bacterial communities were used in these experiments, comprising 14 different sac...

journal_title：Journal of applied microbiology

authors： Newton DF,Cummings JH,Macfarlane S,Macfarlane GT

更新日期：1998-08-01 00:00:00

abstract：AIMS:This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional culture method. METHODS AND RESULTS:Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water sa...

journal_title：Journal of applied microbiology

authors： Bonetta S,Bonetta S,Ferretti E,Balocco F,Carraro E

更新日期：2010-05-01 00:00:00

abstract：AIM:To study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous Enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. METHODS AND RESULTS:Six Enterococcus faecalis strains were paired with 15 other microbial isolates to form mix...

journal_title：Journal of applied microbiology

authors： van Merode AE,Pothoven DC,van der Mei HC,Busscher HJ,Krom BP

更新日期：2007-05-01 00:00:00

abstract：AIMS:To achieve reliable detection of methicillin resistance in clinical isolates of coagulase-negative staphylococci. METHODS AND RESULTS:Strains (105) were evaluated by normatized antimicrobial susceptibility methods, and for the presence of the methicillin resistance-determining mecA gene, using the polymerase chai...

journal_title：Journal of applied microbiology

authors： Bogado I,Sutich E,Krapp A,Marchiaro P,Marzi M,Putero J,Carrillo N

更新日期：2001-08-01 00:00:00

abstract：:The purpose of this article is to review coculture fermentations in industrial biotechnology. Examples for the advantageous utilization of cocultures instead of single cultivations include the production of bulk chemicals, enzymes, food additives, antimicrobial substances and microbial fuel cells. Coculture fermentati...

journal_title：Journal of applied microbiology

authors： Bader J,Mast-Gerlach E,Popović MK,Bajpai R,Stahl U

更新日期：2010-08-01 00:00:00

abstract：:Cerein 7 is a peptidic antibiotic produced by Bacillus cereus Bc7 (CECT 5148) at the end of exponential growth but before sporulation onset. Cerein 7 has a broad spectrum of antibacterial activity against Gram-positive bacteria, but it is inactive against Gram-negative bacteria. The sequence of its amino-terminal end ...

journal_title：Journal of applied microbiology

authors： Oscáriz JC,Pisabarro AG

更新日期：2000-08-01 00:00:00

abstract：AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...

journal_title：Journal of applied microbiology

authors： Nigatu A

更新日期：2000-12-01 00:00:00