Inhibition of Bacillus licheniformis LMG 19409 from ropy cider by enterocin AS-48.

Abstract:

AIMS:To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. METHODS AND RESULTS:Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly inactivated in MRS-G by 0.5 microg ml(-1)AS-48 and in fresh-made apple juice by 3 microg ml(-1). Concentration-dependent inactivation of this bacterium in two commercial apple ciders (A and B) stored at 4, 15 and 30 degrees C for 15 days was also demonstrated. Counts from heat-activated endospores in cider A plus AS-48 decreased very slowly. Application of combined treatments of heat (95 degrees C) and enterocin AS-48 reduced the time required to achieved complete inactivation of intact spores in cider A to 4 min for 6 microg ml(-1) and to 1 min for 12 microg ml(-1). D and z values also decreased as the bacteriocin concentration increased. CONCLUSION:Enterocin AS-48 can inhibit ropy-forming B. licheniformis in apple cider and increase the heat sensitivity of spores. SIGNIFICANCE AND IMPACT OF THE STUDY:Results from this study support the potential use of enterocin AS-48 to control B. licheniformis in apple cider.

journal_name

J Appl Microbiol

authors

Grande MJ,Lucas R,Abriouel H,Valdivia E,Ben Omar N,Maqueda M,Martínez-Cañamero M,Gálvez A

doi

10.1111/j.1365-2672.2006.02942.x

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

422-8

issue

2

eissn

1364-5072

issn

1365-2672

pii

JAM2942

journal_volume

101

pub_type

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