Abstract：
AIM:To evaluate the activity and effectiveness of impregnated central venous catheters (CVC) against Klebsiella pneumoniae biofilms. METHODS AND RESULTS:The antimicrobial activity and durability of impregnated-CVCs were evaluated over time and the size of zones of inhibition (ZI) was measured. Biofilm formation was observed by quantitative culture and also by scanning electron microscopy. The catheters impregnated with chlorhexidine/silver sulfadiazine (CHX/SS) reduced bacteria counts by 0·3 log and were most effective (P < 0·01) against Klebsiella pneumoniae biofilms N-acetylcysteine/levofloxacin (NAC/LEV) catheters. It was observed that the catheter impregnated with NAC/LEV had initially the largest average ZI size being statistically significant (P < 0·01). The NAC/LEV combination remained active until day 30, whereas the combination of CHX/SS was completely inactivated from day 15 on. CONCLUSIONS:The NAC/LEV combination showed greater durability on the catheters, but it was the CHX/SS combination that had the greater initial efficacy in bacterial inhibition. It was also observed that NAC/LEV-impregnated catheters do not prevent the emergence of resistant subpopulations inside the inhibition halos during antimicrobial susceptibility tests. SIGNIFICANCE AND IMPACT OF THE STUDY:Our results highlighted that the in vitro efficacy of antimicrobial-impregnated CVCs is limited by time and that their colonization occurred earlier than expected. Our data also demonstrated that NAC/LEV remained active until day 30 of evaluation and CHX/SS combination was completely inactivated from day 15 on. Our findings suggested that implantable devices should be carefully used by medical community.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
de Sousa JKT,Haddad JPA,de Oliveira AC,Vieira CD,Dos Santos SGdoi
10.1111/jam.14372subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1018-1027issue
4eissn
1364-5072issn
1365-2672journal_volume
127pub_type
杂志文章abstract：AIM:To develop in vitro assays for comparing the antagonistic properties and anti-oxidative activity of probiotic Lactobacillus and Bifidobacterium strains against various entero- and urinary pathogens. METHODS AND RESULTS:The antagonistic activity of five probiotic lactobacilli (Lactobacillus rhamnosus GG, Lactobacil...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02857.x
更新日期：2006-06-01 00:00:00
abstract：AIMS:The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14283
更新日期：2019-07-01 00:00:00
abstract：:The hygienic performances of the carcass dressing processes at 10 beef packing plants were assessed from small sets of microbiological data. For each process, a single sample was obtained from a randomly selected site on each of 25 randomly selected beef sides leaving the process. In addition, during a period of about...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00441.x
更新日期：1998-06-01 00:00:00
abstract：AIMS:Cloning and expression of a new cry1Ia-type gene of Bacillus thuringiensis. METHODS AND RESULTS:PCR amplification, using gene cry1I-specific primers revealed the presence of such a gene in the strain BNS3 of Bacillus thuringiensis subsp. kurstaki. The cloning and sequencing from BNS3 of the cry1Ia-type gene, call...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.t01-1-01947.x
更新日期：2003-01-01 00:00:00
abstract：:Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.00977.x
更新日期：2000-03-01 00:00:00
abstract：AIMS:To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. METHODS AND RESULTS:Processed semen was spiked with known amounts of MAP. Semen from different bulls as well as semen of diff...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02924.x
更新日期：2006-05-01 00:00:00
abstract：:New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E-78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram-negative test organism, Pantoea agglomerans (Enterobacter...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1999.00632.x
更新日期：1999-01-01 00:00:00
abstract：:A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00455.x
更新日期：1998-06-01 00:00:00
abstract：:Grab samples of drinking water collected from reservoirs and from creeks flowing over pristine land, farmland or land having mixed use were analysed for their physicochemical and microbiological characteristics. A significant difference between sites for conductivity and sites for pH was noted using a two-way ANOVA. N...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00390.x
更新日期：1998-04-01 00:00:00
abstract：AIMS:The aim of this study was to investigate changes in Salmonella and total viable count (TVC) survival on beef carcass surfaces stored for 72 h under different combinations of relative humidity (i.e. RH 75% or 96%) and temperature (5 degrees C or 10 degrees C). METHODS AND RESULTS:The influence of low water activit...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03989.x
更新日期：2009-01-01 00:00:00
abstract：AIMS: In this study, we compare seven different methods which have been designed or modified to extract total DNA from raw milk and raw milk cheese with a view to its subsequent use for the PCR of bacterial DNA. MATERIALS AND RESULTS: Seven extraction methods were employed to extract total DNA from these foods, and ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05294.x
更新日期：2012-07-01 00:00:00
abstract：AIMS:To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus (TEV), Potato virus Y (PVY) and Tobacco vein banding mosaic virus (TVBMV). METHODS AND RESULTS:Specific primer and probe combinations for TEV and TVBMV were developed from the coa...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12071
更新日期：2013-02-01 00:00:00
abstract：AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04561.x
更新日期：2010-04-01 00:00:00
abstract：AIM:To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS:Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying pla...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01768.x
更新日期：2002-01-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03255.x
更新日期：2007-08-01 00:00:00
abstract：AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04755.x
更新日期：2010-10-01 00:00:00
abstract：:The inactivation of Bacillus subtilis spores by ultrasonic treatments under static pressure (Mano-Sonication, MS) and a combined MS/heat treatment (Mano-Thermo-Sonication) was investigated. The sporicidal effect of MS treatments depended on static pressure, amplitude of ultrasonic waves and treatment temperature. At 7...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00593.x
更新日期：1998-11-01 00:00:00
abstract：AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13516
更新日期：2017-09-01 00:00:00
abstract：AIMS:To assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of Satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03694.x
更新日期：2008-06-01 00:00:00
abstract：AIM:The aim of this study was to develop and evaluate bacterial-fungal communities to deconstruct switchgrass to biofuel precursors. METHODS AND RESULTS:Bacterial-fungal consortia, mesophilic (25°C) and thermophilic (50°C), were enriched from switchgrass bales from which enzyme mixtures were used to deconstruct delign...
journal_title：Journal of applied microbiology
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doi：10.1111/jam.13295
更新日期：2017-04-01 00:00:00
abstract：AIMS:To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS AND RESULTS:Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic count...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03527.x
更新日期：2008-01-01 00:00:00
abstract：:Of 10 strains of mannanase-producing anaerobic bacteria isolated from soils and methanogenic sludges, Clostridium tertium KT-5A, which was isolated from lotus soil, produced high amounts of extracellular beta-1,4-mannanase. The isolate was an aerotolerant anaerobe without quinon systems; the cell growth cultivated wit...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00349.x
更新日期：1998-03-01 00:00:00
abstract：AIMS:To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. METHODS AND RESULTS:Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofil...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04152.x
更新日期：2009-06-01 00:00:00
abstract：AIMS:To enhance the recovery of f(2) bacteriophage and poliovirus by an established method based on the adsorption to and elution from positively-charged Al(OH)(3)-treated silica gel. METHODS AND RESULTS:Polyaluminum Chloride (PAC) was added to water samples to neutralize the negatively charged materials, which can re...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04041.x
更新日期：2009-02-01 00:00:00
abstract：AIMS:To develop a predictive model for Escherichia coli using deep neural networks. METHODS AND RESULTS:Batch experiments are conducted at different temperatures closer to optimum value (36·5°C, 37°C, 37·5°C, 38°C and 38·5°C) to obtain the growth curves of E .coli K-12. Two primary models namely modified Gompertz and ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14901
更新日期：2020-10-16 00:00:00
abstract：:The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.01054.x
更新日期：2000-05-01 00:00:00
abstract：AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12351
更新日期：2014-01-01 00:00:00
abstract：AIMS:To obtain the high-yield strain of fusidic acid, which is produced from fungus Fusidium coccineum and is the only fusidane-type antibiotic that has been used clinically, and confirm the changes in the transcription levels involved in increasing its production. METHODS AND RESULTS:By using the atmospheric and room...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14797
更新日期：2020-07-30 00:00:00
abstract：AIMS:Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). METHODS AND RESULTS:Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. B...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12546
更新日期：2014-08-01 00:00:00
abstract：AIMS:To determine the effects of supplemented copper (Cu2+) on growth and viability of strains used as starters and adjunct cultures for Emmental cheese manufacture. METHODS AND RESULTS:Thirteen strains belonging to Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus rhamnosus, Streptococcus thermophilu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03849.x
更新日期：2008-10-01 00:00:00
