Determination of antimicrobial resistance and virulence gene signatures in surface water isolates of Escherichia coli.

Abstract:

AIMS:To determine the occurrence of Escherichia coli harbouring virulence markers of shiga- or entero-toxins and resistance to antimicrobials in surface waters. METHODS AND RESULTS:Surface water samples were collected at six locations of the river Gomti. E. coli isolates (n = 90) were characterized for their pathogenic potential using polymerase chain reaction to detect virulence genes as well as their sensitivity to antimicrobial agents using disc diffusion methods. In this study, 57.8% of E. coli isolates exhibited resistance to three or more antimicrobial agents. Sensitivity to cephotaxime, gentamicin and norfloxacin was observed in 7.8%, 48.9% and 77.8% of isolates, respectively. Both stx1 and stx2 genes were present in 15.6% of isolates while remaining isolates had either stx1 (17.8%) or stx2 (6.7%). The stx1 gene (33.3%) was more prevalent than stx2 (22.2%). The results indicate that the LT1 and ST1 genes were positive in 21.2% of isolates. CONCLUSIONS:The presence of multi-drug resistance and virulence genes in E. coli isolated from surface water being used for domestic and recreational purposes may result in waterborne outbreaks. SIGNIFICANCE AND IMPACT OF THE STUDY:The data will be useful in monitoring surface waters for forecasting and management of waterborne outbreaks.

journal_name

J Appl Microbiol

authors

Ram S,Vajpayee P,Tripathi U,Singh RL,Seth PK,Shanker R

doi

10.1111/j.1365-2672.2008.03879.x

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

1899-908

issue

6

eissn

1364-5072

issn

1365-2672

pii

JAM3879

journal_volume

105

pub_type

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