Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus.

Abstract:

AIMS:To evaluate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. METHODS AND RESULTS:Specific IgY was produced by immunizing hens with formaldehyde-killed Staph. aureus, using a bacterial strain known to cause mastitis. The IgY, of 94% purity, was obtained from yolks by water dilution, salt precipitations, ultrafiltration and gel filtration. ELISA indicated that the IgY produced was specific to the antigen and five Staph. aureus isolates obtained from mastitic cows. The growth of Staph. aureus was inhibited by specific IgY at concentrations from 1 to 10 mg ml(-1) in a dose-dependent manner. The phagocytosis of Staph. aureus by milk macrophages was enhanced in the presence of specific IgY with the highest phagocytic percentage being 30% higher than that without IgY (P < 0.05). CONCLUSIONS:The specific IgY against mastitis-causing Staph. aureus inhibited the growth of Staph. aureus and enhanced the phagocytosis of Staph. aureus by milk macrophages. SIGNIFICANCE AND IMPACT OF THE STUDY:Specific IgY would be a potential treatment for bovine mastitis.

journal_name

J Appl Microbiol

authors

Zhen YH,Jin LJ,Guo J,Li XY,Li Z,Fang R,Xu YP

doi

10.1111/j.1365-2672.2008.03920.x

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1529-35

issue

5

eissn

1364-5072

issn

1365-2672

pii

JAM3920

journal_volume

105

pub_type

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