Diversity of spore germination in response to inosine and L-alanine and its interaction with NaCl and pH in the Bacillus cereus group.

Abstract:

AIMS:Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS:B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 strains taken to illustrate the diversity of the B. cereus group showed ranging germination capacities. Two strains never germinated in the presence of l-alanine, at any of the germinant concentrations tested. Both the extent and rate of spore germination were affected by low pH and high NaCl concentration, but differently according to the strain. CONCLUSIONS:A broad diversity was observed in nutrient-triggered spore germination among the members of the B. cereus group. Spore germination of some strains occurred at low concentrations of inosine or l-alanine, suggesting high receptor sensitivity to germinants. The activity of these receptors was also affected by pH or high NaCl concentration. SIGNIFICANCE AND IMPACT OF THE STUDY:The greater ability of some strains to germinate in response to l-alanine and inosine is one criterion among others for B. cereus strain selection in food processing or storage studies, before confirmation in complex food or laboratory media. The diversity in response to germinants found among the B. cereus strains suggests a differential expression and (or) absence of some germination genes involved in the response, mainly to l-alanine.

journal_name

J Appl Microbiol

authors

Broussolle V,Gauillard F,Nguyen-The C,Carlin F

doi

10.1111/j.1365-2672.2008.03847.x

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1081-90

issue

4

eissn

1364-5072

issn

1365-2672

pii

JAM3847

journal_volume

105

pub_type

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