Abstract:
AIMS:Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS:B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 strains taken to illustrate the diversity of the B. cereus group showed ranging germination capacities. Two strains never germinated in the presence of l-alanine, at any of the germinant concentrations tested. Both the extent and rate of spore germination were affected by low pH and high NaCl concentration, but differently according to the strain. CONCLUSIONS:A broad diversity was observed in nutrient-triggered spore germination among the members of the B. cereus group. Spore germination of some strains occurred at low concentrations of inosine or l-alanine, suggesting high receptor sensitivity to germinants. The activity of these receptors was also affected by pH or high NaCl concentration. SIGNIFICANCE AND IMPACT OF THE STUDY:The greater ability of some strains to germinate in response to l-alanine and inosine is one criterion among others for B. cereus strain selection in food processing or storage studies, before confirmation in complex food or laboratory media. The diversity in response to germinants found among the B. cereus strains suggests a differential expression and (or) absence of some germination genes involved in the response, mainly to l-alanine.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Broussolle V,Gauillard F,Nguyen-The C,Carlin Fdoi
10.1111/j.1365-2672.2008.03847.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
1081-90issue
4eissn
1364-5072issn
1365-2672pii
JAM3847journal_volume
105pub_type
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