Abstract:
AIMS:To assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, Thermophilin 1277, production by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277, which was produced by S. thermophilus SBT1277, showed an antimicrobial activity against several lactic acid bacteria and food spoilage bacteria including Clostridium butylicum, C. sprogenes and Bacillus cereus. Thermophilin 1277 was inactivated by proteinase K. Heating treatment did not affect the antimicrobial activity. The partially purified Thermophilin 1277 had an apparent molecular mass of 3.7 kDa. N-terminal sequence analysis revealed 15 amino acid residues that correspond with amino acid sequence of the lantibiotics bovicin HJ50 produced by Streptococcus bovis HJ50. The effects of culture condition for the bacteriocin production by S. thermophilus SBT1277 were studied. During the batch fermentation, Thermophilin 1277 was produced in M17 broth, but no bacteriocin production occurred in the sucrose-tryptone (ST) broth. Bacteriocin production was detected in pH controlled ST broth at pH values of 5.5-6.5. CONCLUSIONS:Thermophilin 1277 production from S. thermophilus strain depended on the culture conditions. Some characters and N-terminal amino acid sequence of Thermophilin 1277 differed from bacteriocins produced by S. thermophilus reported previously. SIGNIFICANCE AND IMPACT OF THE STUDY:Streptococcus thermophilus SBT1277 or its bacteriocin which has a wide inhibitory spectrum has a potential use as a biopreservative in dairy products.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Kabuki T,Uenishi H,Watanabe M,Seto Y,Nakajima Hdoi
10.1111/j.1365-2672.2006.03159.xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
971-80issue
4eissn
1364-5072issn
1365-2672pii
JAM3159journal_volume
102pub_type
杂志文章abstract:AIMS:The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. METHODS A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14213
更新日期:2019-05-01 00:00:00
abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03042.x
更新日期:2006-12-01 00:00:00
abstract:AIMS:Coagulase-negative Staphylococcus xylosus strains are used as starter organisms for sausage fermentation. As those strains have to cope with low pH-values during fermentation, the aim of this study was to identify the acid adaptation mechanisms of S. xylosus TMW 2.1523 previously isolated from salami. METHODS AND...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14224
更新日期:2019-05-01 00:00:00
abstract::A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01150.x
更新日期:2000-10-01 00:00:00
abstract:AIMS:(i) To determine the aflatoxin B1 (AFB1 ) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB1 at each gastrointestinal tract (GIT-saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14101
更新日期:2019-01-01 00:00:00
abstract:AIMS:To investigate the genetic determinates for conjugated linolenic acid (CLNA) production in Lactobacillus plantarum ZS2058, a high CLNA producer. METHODS AND RESULTS:After culturing with α-linolenic acid (ALA) in the medium, the fatty acid compositions of supernatant fluid and cell pellets were analysed via GC-MS....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14466
更新日期:2020-01-01 00:00:00
abstract:AIMS:To investigate the effect of silver-based antimicrobial material incorporated in the inner liners of refrigerators on food safety and quality. METHODS AND RESULTS:In the first stage, the bactericidal effect was tested in the laboratory. Silver-containing samples and control plates were inoculated with different b...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03727.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:To compare the production of recombinant cellulase enzymes in two Saccharomyces species so as to ascertain the most suitable heterologous host for the degradation of cellulose-based biomass and its conversion into bioethanol. METHOD AND RESULTS:cDNA copies of genes representing the three major classes of cellulas...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12494
更新日期:2014-07-01 00:00:00
abstract:AIM:To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease. METHODS AND RESULTS:Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and fiel...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03611.x
更新日期:2008-04-01 00:00:00
abstract::Sustainable economic and industrial growth is the need of the hour and it requires renewable energy resources having better performance and compatibility with existing fuel infrastructure from biological routes. Isoprenoids (C ≥ 5) can be a potential alternative due to their diverse nature and physiochemical propertie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.12871
更新日期:2015-09-01 00:00:00
abstract:AIMS:To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. METHODS AND RESULTS:A multiplex probe array was designed for genotypic test to simultaneously screen the mutations ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03350.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan equine encephalitis virus (VEEV), on two material types. METHODS AND RESULTS:Francisella tularensis (F.t.) and VEEV were inoculated (c. 1 × 108 colony-form...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14532
更新日期:2020-04-01 00:00:00
abstract:AIMS:To evaluate and compare the reductions of human viruses and F-specific coliphages in a full-scale wastewater treatment plant based on the quantitative PCR (qPCR) and plate count assays. METHODS AND RESULTS:A total of 24 water samples were collected from four locations at the plant, and the relative abundance of h...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12051
更新日期:2013-02-01 00:00:00
abstract:AIMS:A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. METHODS AND RESULTS:The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of time. The microcolonie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01230.x
更新日期:2001-02-01 00:00:00
abstract:AIM:This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. METHODS AND RESULTS:Biosurfactant named 'Uzmaq' was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12929
更新日期:2015-10-01 00:00:00
abstract:AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04393.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13615
更新日期:2018-01-01 00:00:00
abstract:AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01310.x
更新日期:2001-05-01 00:00:00
abstract:AIMS:This study was applied to evaluate the usefulness of a high-throughput sample preparation protocol prior to the application of quantitative real-time PCR (qPCR) for the early diagnosis of bloodstream and pyogenic infections in humans and animals compared to matrix-assisted laser desorption ionization-time of fligh...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14862
更新日期:2020-10-18 00:00:00
abstract:AIMS:The purpose of this study was to investigate the inactivation kinetics of Staphylococcus aureus in a ham model system by high hydrostatic pressure at ambient (25 degrees C) and selected temperatures (45, 55 degrees C). Selective [Baird Parker (BP) agar] and nonselective [brain heart infusion (BHI) agar] growth med...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03698.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14866
更新日期:2020-10-07 00:00:00
abstract:AIMS:To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS:The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campyloba...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02616.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics Lactobacillus paracasei subsp. paracasei B112, DC205, DC215 and DC412 strains in the gut mucosa. METHODS AND RESULTS:Only the DC412 strain interacted strongly with the cells forming the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04421.x
更新日期:2010-01-01 00:00:00
abstract::Thirty-eight Lactococcus strains, isolated from raw milk produced in two dairy areas in Normandy, were identified at the phenotypic level. Only Lactococcus lactis strains with the lactis phenotype were found in the milk samples. Most strains fermented lactose (97%) and showed proteinase activity (76%). Isolates were c...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1998.tb05264.x
更新日期:1998-12-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14520
更新日期:2020-03-01 00:00:00
abstract:AIM:Evaluation of the thermal and physical conditions for inactivation of adenovirus (AdV), porcine sapelovirus 1 (PSV1) and the economically important viruses porcine epidemic diarrhoea virus (PEDV) and porcine circovirus 2 (PCV2) in the production of spray-dried porcine plasma (SDPP). METHODS AND RESULTS:Citrate-tre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14235
更新日期:2019-06-01 00:00:00
abstract:AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12767
更新日期:2015-05-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02491.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To quantify the ability of 136 lactic acid bacteria (LAB), isolated from wine, to produce histamine and to identify the bacteria responsible for histamine production in wine. METHODS AND RESULTS:A qualitative method based on pH changes in a plate assay was used to detect wine strains capable of producing high lev...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02633.x
更新日期:2005-01-01 00:00:00
abstract::Ecological and physiological effects of the sulphate-reducing bacterium (SRB) Desulfovibrio desulfuricans on other intestinal organisms were investigated in anaerobic chemostats (dilution rate approximately 0.2 h-1). Reproducible defined bacterial communities were used in these experiments, comprising 14 different sac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00522.x
更新日期:1998-08-01 00:00:00