Analysis of environmental Escherichia coli isolates for virulence genes using the TaqMan PCR system.

abstract：AIM:To investigate the aerobiology of the environment around mechanically ventilated broiler sheds with the aim of understanding dispersion in the surrounding environment. METHODS AND RESULTS:Aerosol samples were collected weekly on four different commercial broiler farms through the cycle of 55 days from 2005 to 2007...

journal_title：Journal of applied microbiology

authors： Chinivasagam HN,Tran T,Maddock L,Gale A,Blackall PJ

更新日期：2010-05-01 00:00:00

abstract：AIMS:Fungal infection is still a life-threatening risk for the immunocompromised population such as AIDS patients and those who receive treatments with immunosuppressors and/or frequent administrations of wide-spectrum antibiotics, which inevitably lead to the drug resistance and unbalanced microflora populations. The ...

journal_title：Journal of applied microbiology

authors： Liu JY,Huang LL,Ye YH,Zou WX,Guo ZJ,Tan RX

更新日期：2006-01-01 00:00:00

abstract：AIMS:To determine the ability of a bismuth thiol to control floc formation in a multispecies population of micro-organisms obtained from the activated sludge unit of a wastewater treatment plant. The molecular level mechanisms by which bismuth-2-3-dimercapto-1-propanol (BisBAL) inhibits bioaggregation are also elucidat...

journal_title：Journal of applied microbiology

authors： Badireddy AR,Chellam S

更新日期：2011-06-01 00:00:00

abstract：AIMS:To obtain data on the efficacy of various liquid and foam decontamination technologies to inactivate Bacillus anthracis Ames and Bacillus subtilis spores on building and outdoor materials. METHODS AND RESULTS:Spores were inoculated onto test coupons and positive control coupons of nine different materials. Six di...

journal_title：Journal of applied microbiology

authors： Wood JP,Choi YW,Rogers JV,Kelly TJ,Riggs KB,Willenberg ZJ

更新日期：2011-05-01 00:00:00

abstract：AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

journal_title：Journal of applied microbiology

authors： Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

更新日期：2010-06-01 00:00:00

abstract：AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...

journal_title：Journal of applied microbiology

authors： Abreu C,Jurgens G,De Marco P,Saano A,Bordalo AA

更新日期：2001-05-01 00:00:00

abstract：AIMS:To study the behaviour of salmonellae inoculated in the dry, raw materials, related to chocolate confectionery manufacture, as affected by the strain, cell preparation method and storage temperature. METHODS AND RESULTS:Outbreak and non outbreak-associated salmonellae were used for the inoculation of cocoa butter...

journal_title：Journal of applied microbiology

authors： Komitopoulou E,Peñaloza W

更新日期：2009-06-01 00:00:00

abstract：AIM:A new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO(3) and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. METHODS AND RESULTS:Two evaluations of EC-Blue-10 were carried out. Firstly, EC-Blue-10 w...

journal_title：Journal of applied microbiology

authors： Kodaka H,Mizuochi S,Saito M,Matsuoka H

更新日期：2008-04-01 00:00:00

abstract：AIMS:To see the possibility of particle size distribution analyser (PSDA) in detecting concentration of lactobacillus contaminants in yeast fermentation. METHODS AND RESULTS:A PSDA was used to rapidly determine the size and concentration of lactobacillus and Saccharomyces cerevisiae. Data showed that the aerodynamic d...

journal_title：Journal of applied microbiology

authors： Kim H,Park K,Oh S,Chang IS

更新日期：2009-11-01 00:00:00

abstract：AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...

journal_title：Journal of applied microbiology

authors： Maluping RP,Ravelo C,Lavilla-Pitogo CR,Krovacek K,Romalde JL

更新日期：2005-01-01 00:00:00

abstract：AIMS:To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. METHODS AND RESULTS:Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly ...

journal_title：Journal of applied microbiology

authors： Grande MJ,Lucas R,Abriouel H,Valdivia E,Ben Omar N,Maqueda M,Martínez-Cañamero M,Gálvez A

更新日期：2006-08-01 00:00:00

abstract：AIMS:The objective of this study was to access APC-effector cell cluster formation in genetically susceptible BALB/c (H-2(d) ) mice infected with highly virulent Moscow strain of ectromelia virus (ECTV-MOS) and estimate of lymphocyte activation based upon expression of CD62L and CD44 molecules. METHODS AND RESULTS:APC...

journal_title：Journal of applied microbiology

authors： Szulc L,Boratynska A,Martyniszyn L,Niemialtowski MG

更新日期：2010-11-01 00:00:00

abstract：:A 210-min-test for the enumeration of Escherichia coli in drinking water is described, based on solid phase cytometry (SPC) and a two-step enzymatic procedure for fluorescence labelling of single cells and small microcolonies. The test involves membrane filtration through a 25-mm black polyester filter, induction of b...

journal_title：Journal of applied microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：2000-09-01 00:00:00

abstract：AIMS:To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. METHODS AND RESULTS:Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragm...

journal_title：Journal of applied microbiology

authors： Arias CR,Welker TL,Shoemaker CA,Abernathy JW,Klesius PH

更新日期：2004-01-01 00:00:00

abstract：AIMS:The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng). ...

journal_title：Journal of applied microbiology

authors： Sharma D,Mal G,Kannan A,Bhar R,Sharma R,Singh B

更新日期：2017-11-01 00:00:00

abstract：:Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

journal_title：Journal of applied microbiology

authors： George I,Petit M,Servais P

更新日期：2000-03-01 00:00:00

abstract：:Pseudomonas fluorescens DR54 showed antagonistic properties against plant pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. Antifungal activity was extractable from spent growth media, and fractionation by semi-preparative HPLC resulted in isolation of an active compound, which was identif...

journal_title：Journal of applied microbiology

authors： Nielsen TH,Christophersen C,Anthoni U,Sørensen J

更新日期：1999-07-01 00:00:00

abstract：AIMS:This study aimed at investigating the use of metal chelators as potential metallo-β-lactamase inhibitors (MBL). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of meropenem was ascertained alone and in combination with various concentrations of macrocyclic (1,4,7- triazacyclononane-1-glutaric acid-...

journal_title：Journal of applied microbiology

authors： Azumah R,Dutta J,Somboro AM,Ramtahal M,Chonco L,Parboosing R,Bester LA,Kruger HG,Naicker T,Essack SY,Govender T

更新日期：2016-04-01 00:00:00

abstract：:A novel antibacterial substance produced by a strain isolated from Bulgarian yellow cheese was characterized. The producer strain was identified by molecular typing to belong to the species Lactobacillus delbrueckii, which is a rare producer of bacteriocins. The inhibitory agent was heat stable and active against lact...

journal_title：Journal of applied microbiology

authors： Miteva V,Ivanova I,Budakov I,Pantev A,Stefanova T,Danova S,Moncheva P,Mitev V,Dousset X,Boyaval P

更新日期：1998-09-01 00:00:00

abstract：AIMS:Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in ...

journal_title：Journal of applied microbiology

authors： Coronel-León J,Marqués AM,Bastida J,Manresa A

更新日期：2016-01-01 00:00:00

abstract：AIMS:To assess antibiotic resistance in airborne bacteria associated with coarse particulate matter (PM10) in an urban area, with specific considerations about the Staphylococcus genus. METHODS AND RESULTS:Disc diffusion test was performed on 243 microbial strains, isolated from PM10 in winter and summer and belonging...

journal_title：Journal of applied microbiology

authors： Gandolfi I,Franzetti A,Bertolini V,Gaspari E,Bestetti G

更新日期：2011-06-01 00:00:00

abstract：:Pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. Among the fungal diseases, blue mold caused by Penicillium expansum, grey mould caused by Botrytis cinerea, Mucor rot caused by Mucor piriformis are common on pear fruits. Other (weak) pathogens like Phi...

journal_title：Journal of applied microbiology

authors： Mari M,Bertolini P,Pratella GC

更新日期：2003-01-01 00:00:00

abstract：AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...

journal_title：Journal of applied microbiology

authors： Guo L,Long M,Huang Y,Wu G,Deng W,Yang X,Li B,Meng Y,Cheng L,Fan L,Zhang H,Zou L

更新日期：2015-07-01 00:00:00

abstract：AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The effica...

journal_title：Journal of applied microbiology

authors： Wang J,Qiu J,Dong J,Li H,Luo M,Dai X,Zhang Y,Leng B,Niu X,Zhao S,Deng X

更新日期：2011-12-01 00:00:00

abstract：AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...

journal_title：Journal of applied microbiology

authors： Crothers-Stomps C,Høj L,Bourne DG,Hall MR,Owens L

更新日期：2010-05-01 00:00:00

abstract：AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...

journal_title：Journal of applied microbiology

authors： Bejaoui H,Mathieu F,Taillandier P,Lebrihi A

更新日期：2004-01-01 00:00:00

abstract：AIMS:Hydrogen peroxide (H2 O2 ) disinfection applications are limited by its rapid inactivation. The aims of this study were to (i) micro-encapsulate H2 O2 in silica hydrogels to obtain controlled release up to 72 h, (ii) test hydrogel antimicrobial activity against four common pathogens and (iii) assess H2 O2 release ...

journal_title：Journal of applied microbiology

authors： Dogan-Guner EM,Mohamed H,Orbey N,Goodyear N

更新日期：2019-03-01 00:00:00

abstract：AIMS:Thymol-rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water solubility and high vapour pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in wate...

journal_title：Journal of applied microbiology

authors： Ghaderi L,Moghimi R,Aliahmadi A,McClements DJ,Rafati H

更新日期：2017-10-01 00:00:00

abstract：AIMS:Chlorophyll is a photosynthetic pigment found in plants and algal organisms and is a bioproduct with human health benefits and a great potential for use in the food industry. The chlorophyll content in microalgae strains varies in response to environmental factors. In this work, we assessed the effect of nitrogen ...

journal_title：Journal of applied microbiology

authors： Ferreira VS,Pinto RF,Sant'Anna C

更新日期：2016-03-01 00:00:00

abstract：AIMS:This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. METHODS AND RESULTS:The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when ...

journal_title：Journal of applied microbiology

authors： Wu Z,Xuanyuan Z,Li R,Jiang D,Li C,Xu H,Bai Y,Zhang X,Turakainen H,Saris PE,Savilahti H,Qiao M

更新日期：2009-01-01 00:00:00