Abstract:
AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enumerated as fluoro-microscopic counts during a 62-operating day period using nucleic acid staining and the direct epifluorescence filter technique. The biofilm development could be separated into three distinct phases: (i) cell attachment, (ii) biofilm establishment and (iii) biofilm maturation. The LC were always dominant (>/=72%) in the biofilm during the establishment phase whereas the average LC fraction decreased to 51% of the total cells in the maturation phase. The concentration of LC and DC was observed to level off after 41 days at 1010 cells per ring. The biofilm thickness and the dry weight increased independently of the cell number during the maturation phase. CONCLUSIONS:After the LC reached a maximum concentration in the biofilm, the biofilm proliferation was only characterized by the accumulation of DC and organic matter. SIGNIFICANCE AND IMPACT OF THE STUDY:The results obtained in the present study are of particular relevance for biofilm mathematical modelling and numerical simulations. They will also be useful to estimate the contribution of the living bacteria within the biofilm in bioprocesses.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Tresse O,Lescob S,Rho Ddoi
10.1046/j.1365-2672.2003.01914.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
849-54issue
5eissn
1364-5072issn
1365-2672pii
1914journal_volume
94pub_type
杂志文章abstract:AIMS:The essential oil of Melaleuca alternifolia (tea tree) has been evaluated as a potential eco-friendly antifungal agent against Botrytis cinerea. In this study, we investigated the antifungal activity and mode of action of tea tree oil (TTO) and its components against B. cinerea. METHODS AND RESULTS:Of the compone...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12939
更新日期:2015-11-01 00:00:00
abstract:AIMS:To provide molecular fingerprinting evidence of the contribution of wildlife vectors in the on-farm epidemiology of Salmonella Enteritidis infections. METHODS AND RESULTS:Salmonella Enteritidis strains were isolated from wildlife and from farm environment samples collected in 10 egg layer farms. Isolates were typ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01924.x
更新日期:2003-01-01 00:00:00
abstract::Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04100.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:Development of a real-time PCR method for the specific detection of Salmonella Dublin. METHODS AND RESULTS:The method was directed towards a Salm. Dublin-specific sequence of the vagC gene on the Salmonella virulence plasmid (pSDV) and towards Salmonella genus-specific sequence of the invA gene, serving as an int...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05378.x
更新日期:2012-09-01 00:00:00
abstract:AIMS:To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. METHODS AND RESULTS:The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied using a microtitre plate...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01858.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To investigate the genetic determinates for conjugated linolenic acid (CLNA) production in Lactobacillus plantarum ZS2058, a high CLNA producer. METHODS AND RESULTS:After culturing with α-linolenic acid (ALA) in the medium, the fatty acid compositions of supernatant fluid and cell pellets were analysed via GC-MS....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14466
更新日期:2020-01-01 00:00:00
abstract:AIMS:To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. METHODS AND RESULTS:Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l(-1) NaCl; N condition) and control (MRS; C condition) conditions were determined by transmissio...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02428.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain). METHODS AND RESULTS:Micro-organisms present in yellow and grey colonizations were determined from DNA analysis...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03594.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:Lactococcus lactis N8 and Saccharomyces boulardii SAA655 were investigated for their ability to synthesize B-vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. METHODS AND RESULTS:In this study, ultra-high performance liquid chromatography and microbiological as...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13453
更新日期:2017-06-01 00:00:00
abstract:AIMS:As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. METHODS AND RESULTS:We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14565
更新日期:2020-05-01 00:00:00
abstract:AIMS:To evaluate the survival of Campylobacter jejuni relative to that of Escherichia coli in groundwater microcosms varying in nutrient composition. METHODS AND RESULTS:Studies were conducted in groundwater and deionized water incubated for up to 470 days at 4 degrees C. Samples were taken for culturable and total ce...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03285.x
更新日期:2007-09-01 00:00:00
abstract:AIMS:To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. METHODS AND RESULTS:The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and sub...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13254
更新日期:2016-11-01 00:00:00
abstract:AIMS:The aim of the study was to check whether two flavanols ((-)-epigallocatechin gallate and (+)-catechin) can ameliorate oxidative stress (OS) accompanying and contributing to the lethal effects of heating (50°C) and freezing-thawing on the yeast Saccharomyces cerevisiae. METHODS AND RESULTS:The flavanols studied i...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14170
更新日期:2019-03-01 00:00:00
abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05277.x
更新日期:2012-05-01 00:00:00
abstract:AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01297.x
更新日期:2001-05-01 00:00:00
abstract:AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04755.x
更新日期:2010-10-01 00:00:00
abstract:AIMS:The outer membrane porin protein (OMPP) of Bordetella bronchiseptica is an important adhesion factor and protective immunogen. The aim of this study was to verify the immunogenicity of recombinant OMPP and its protective efficacy against a lethal challenge with B. bronchiseptica in rabbits. METHODS AND RESULTS:So...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14451
更新日期:2019-12-01 00:00:00
abstract:AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14790
更新日期:2020-07-20 00:00:00
abstract:AIM:This study aimed to evaluate the patterns and potential risk factors associated with the occurrence of Salmonella sp. and Escherichia coli in selected United States swine feed mills. METHODS AND RESULTS:A total of 405 samples were collected during fall 2018, spring and summer 2019 from selected sites including flo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14758
更新日期:2020-12-01 00:00:00
abstract:AIMS:To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, haemagglutinin 1 (HA1 ). METHODS AND RESULTS:The mucosal and systemic immune response...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12325
更新日期:2013-12-01 00:00:00
abstract:AIMS:Application of autochthonous strain Kocuria rosea KDF3 and its protease KP3 as adjuncts for acceleration of Kedong sufu ripening. METHODS AND RESULTS:Kedong sufu was manufactured using autochthonous cultures (batch A), K. rosea KDF3 plus autochthonous cultures (batch B) and protease KP3 plus autochthonous culture...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12433
更新日期:2014-04-01 00:00:00
abstract:AIM:This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. METHODS AND RESULTS:Biosurfactant named 'Uzmaq' was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12929
更新日期:2015-10-01 00:00:00
abstract:AIMS: To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. METHODS AND RESULTS: The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.04964.x
更新日期:2011-04-01 00:00:00
abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14216
更新日期:2019-05-01 00:00:00
abstract:AIMS:To investigate the conditions that promote the expression of cyanide-resistant respiration (CRR) in the spoilage yeasts Pichia membranifaciens and Debaryomyces hansenii. METHODS AND RESULTS:CRR was detected by sensitivity of oxygen consumption to salicylhydroxamic acid. It was absent in both yeasts in the early e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01992.x
更新日期:2003-01-01 00:00:00
abstract::The hygienic performances of the carcass dressing processes at 10 beef packing plants were assessed from small sets of microbiological data. For each process, a single sample was obtained from a randomly selected site on each of 25 randomly selected beef sides leaving the process. In addition, during a period of about...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00441.x
更新日期:1998-06-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01342.x
更新日期:2001-07-01 00:00:00
abstract:AIMS:Litopenaeus vannamei do not have an adaptive immune response system. White spot syndrome virus (WSSV) is the most severe pathogen in shrimps. Bacillus subtilis spores carrying heterologous antigens on its surface have been evaluated as a vaccine inducing specific systemic responses on vertebrates. Orally administr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12550
更新日期:2014-08-01 00:00:00
abstract:AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01310.x
更新日期:2001-05-01 00:00:00
abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01590.x
更新日期:2002-01-01 00:00:00