Chaperone mediated solubilization of 69-kDa recombinant maltodextrin glucosidase in Escherichia coli.

abstract：AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination ...

journal_title：Journal of applied microbiology

authors： Buhr TL,Minter ZA,Kennihan NL,Young AA,Borgers-Klonkowski EL,Osborn EB,Bohmke MD,Hamilton SM,Kimani MB,Miller CT,Mackie RS,Innocenti JM,Bensman MD,Lilly SD

更新日期：2020-01-01 00:00:00

abstract：AIMS:To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. METHODS AND RESULTS:Flo...

journal_title：Journal of applied microbiology

authors： Tian X,Shao L,Yu Q,Liu Y,Li X,Dai R

更新日期：2019-11-01 00:00:00

abstract：AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

journal_title：Journal of applied microbiology

authors： Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

更新日期：2010-06-01 00:00:00

abstract：AIMS:The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. METHODS AND RESULTS:Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydroly...

journal_title：Journal of applied microbiology

authors： Aires A,Mota VR,Saavedra MJ,Rosa EA,Bennett RN

更新日期：2009-06-01 00:00:00

abstract：AIMS:To isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (SPs) in sheep dips. METHODS AND RESULTS:SP-degrading bacteria were isolated from a mixed soil sample consisting of garden soil and soils from farms where SPs had been used. The two largest in size were then iden...

journal_title：Journal of applied microbiology

authors： Grant RJ,Daniell TJ,Betts WB

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...

journal_title：Journal of applied microbiology

authors： Verma T,Garg SK,Ramteke PW

更新日期：2009-11-01 00:00:00

abstract：AIMS:To identify active phenolic constituents in muscadine grape skin (MGS) extracts and determine interactions among compounds while further exploring their anti-Helicobacter pylori potential in vitro. METHODS AND RESULTS:The inhibitory effects of quercetin and resveratrol, active polyphenols identified in MGS extrac...

journal_title：Journal of applied microbiology

authors： Brown JC,Jiang X

更新日期：2013-04-01 00:00:00

abstract：:A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...

journal_title：Journal of applied microbiology

authors： Arino S,Marchal R,Vandecasteele JP

更新日期：1998-05-01 00:00:00

abstract：AIMS:The present work tests the feasibility of the isothermal microcalorimetry method to study the performance of individual lactic acid bacteria during solid-state fermentation in rye sourdough. Another aim was to elucidate the key factors leading to the formation of different microbial consortia in laboratory and ind...

journal_title：Journal of applied microbiology

authors： Mihhalevski A,Sarand I,Viiard E,Salumets A,Paalme T

更新日期：2011-02-01 00:00:00

abstract：AIMS:To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. METHODS AND RESULTS:Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combinati...

journal_title：Journal of applied microbiology

authors： Maré L,Wolfaardt GM,Dicks LM

更新日期：2006-04-01 00:00:00

abstract：:Biocides (antiseptics and disinfectants) are widely used in hospitals and pharmaceutical industries for contamination control. The emergence of reduced susceptibility to biocides is the major concern and this is caused by various factors, among which plasmid-mediated resistance is common. Many publications describe th...

journal_title：Journal of applied microbiology

authors： Vijayakumar R,Sandle T

更新日期：2019-04-01 00:00:00

abstract：AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...

journal_title：Journal of applied microbiology

authors： Purchase D,Scholes LN,Revitt DM,Shutes RB

更新日期：2009-04-01 00:00:00

abstract：AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

journal_title：Journal of applied microbiology

authors： Wang L,Li Y,Mustapha A

更新日期：2009-11-01 00:00:00

abstract：AIM:To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS:The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated...

journal_title：Journal of applied microbiology

authors： Ercan UK,Sen B,Brooks AD,Joshi SG

更新日期：2018-08-01 00:00:00

abstract：AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...

journal_title：Journal of applied microbiology

authors： Barbe JC,De Revel G,Joyeux A,Bertrand A,Lonvaud-Funel A

更新日期：2001-01-01 00:00:00

abstract：AIMS:To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS:The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campyloba...

journal_title：Journal of applied microbiology

authors： Jensen AN,Andersen MT,Dalsgaard A,Baggesen DL,Nielsen EM

更新日期：2005-01-01 00:00:00

abstract：:The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...

journal_title：Journal of applied microbiology

authors： Boumahdi M,Mary P,Hornez J

更新日期：1999-10-01 00:00:00

abstract：AIMS:To characterize Lactobacillus strains with EPS-producing ability compared with non-EPS-producing lactobacilli of the same species for technological performance including simulated gastrointestinal tract (GIT) conditions. METHODS AND RESULTS:Characterization of EPS-producing Lactobacillus mucosae DPC 6426 in detai...

journal_title：Journal of applied microbiology

authors： London LE,Price NP,Ryan P,Wang L,Auty MA,Fitzgerald GF,Stanton C,Ross RP

更新日期：2014-08-01 00:00:00

abstract：AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...

journal_title：Journal of applied microbiology

authors： Sui J,Leighton S,Busta F,Brady L

更新日期：2002-01-01 00:00:00

abstract：AIMS:One of the major limitations to the production of alfalfa (Medicago sativa) is the fungus Phoma medicaginis, which infects alfalfa and causes leaf spots. This study aims to understand alfalfa's response to P. medicaginis infection, the colonization of arbuscular mycorrhizal fungus (AMF) and the effect of AMF on pl...

journal_title：Journal of applied microbiology

authors： Li Y,Duan T,Nan Z,Li Y

更新日期：2019-07-16 00:00:00

abstract：AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...

journal_title：Journal of applied microbiology

authors： Wang L,Hu X,Tao G,Wang X

更新日期：2012-05-01 00:00:00

abstract：AIMS:To evaluate ATP bioluminescence-based hygiene monitoring systems under conditions relevant to fish processing environments. METHODS AND RESULTS:The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and...

journal_title：Journal of applied microbiology

authors： Møretrø T,Normann MA,Saebø HR,Langsrud S

更新日期：2019-07-01 00:00:00

abstract：AIMS:To monitor seasonal variations in the microbiology of maize silage and to determine whether the risk of fungal spoilage varies during whole-year storage. METHODS AND RESULTS:A continuous survey of 20 maize silage stacks was conducted over a period from three to 11 months after ensiling. Filamentous fungi, yeasts ...

journal_title：Journal of applied microbiology

authors： Storm IM,Kristensen NB,Raun BM,Smedsgaard J,Thrane U

更新日期：2010-09-01 00:00:00

abstract：:An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective cultu...

journal_title：Journal of applied microbiology

authors： Brook MD,Currie B,Desmarchelier PM

更新日期：1997-05-01 00:00:00

abstract：:Grab samples of drinking water collected from reservoirs and from creeks flowing over pristine land, farmland or land having mixed use were analysed for their physicochemical and microbiological characteristics. A significant difference between sites for conductivity and sites for pH was noted using a two-way ANOVA. N...

journal_title：Journal of applied microbiology

authors： Thurman R,Faulkner B,Veal D,Cramer G,Meiklejohn M

更新日期：1998-04-01 00:00:00

abstract：AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...

journal_title：Journal of applied microbiology

authors： Davis KC,Nakatsu CH,Turco R,Weagant SD,Bhunia AK

更新日期：2003-01-01 00:00:00

abstract：:Pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. Among the fungal diseases, blue mold caused by Penicillium expansum, grey mould caused by Botrytis cinerea, Mucor rot caused by Mucor piriformis are common on pear fruits. Other (weak) pathogens like Phi...

journal_title：Journal of applied microbiology

authors： Mari M,Bertolini P,Pratella GC

更新日期：2003-01-01 00:00:00

abstract：AIM:To determine if disinfecting footbaths in the food industry were contaminated with bacteria and to characterize some of the bacteria present. METHODS AND RESULTS:Bacterial strains were isolated from disinfecting footbaths containing TEGO 103G (amphoteric disinfectant) or TP-99 (alkyl amino acetate-based disinfecta...

journal_title：Journal of applied microbiology

authors： Langsrud S,Møretrø T,Sundheim G

更新日期：2003-01-01 00:00:00

abstract：AIMS:To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. METHODS AND RESULTS:After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated Bacillu...

journal_title：Journal of applied microbiology

authors： Wongputtisin P,Khanongnuch C,Khongbantad W,Niamsup P,Lumyong S

更新日期：2012-10-01 00:00:00

abstract：AIMS:To isolate an iron-reducing bacterium and examine its ability of Fe(III) oxide reduction and dechlorination. METHODS AND RESULTS:A fermentative facultative anaerobe, strain L17 isolated from subterranean sediment, can reduce Fe(III) oxides and carbon tetrachloride (CT). It was identified as Klebsiella pneumoniae ...

journal_title：Journal of applied microbiology

authors： Li XM,Zhou SG,Li FB,Wu CY,Zhuang L,Xu W,Liu L

更新日期：2009-01-01 00:00:00